FastQCFastQC Report
Mon 6 Feb 2017
SRR2077095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511254
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79000.5227446875244003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50340.33310085531618117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45580.3016038336374957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41830.2767900035334894No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT30160.19956936424982166No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT29030.19209213011181442No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA28500.18858510879044818No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA27690.18322532148798282No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26330.17422617243692987No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT26170.17316744901915893No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA25320.1675429808622508No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG24710.1635065978319991No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG24630.16297723612311366No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT23980.15867617223841923No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT21390.14153808691325218No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC20290.134259363416077No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC20000.13234042722136716No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC19350.12803936333667273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19090.12631893778279496No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA18930.12526021436502402No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG18480.12228255475254327No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA18250.12076063983949754No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA17230.11401127805120781No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC16900.11182766100205524No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC16730.11070276737067362No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC16490.10911468224401723No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC16380.1083868098942997No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT16370.10832063968068903No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG16210.10726191626291809No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG15890.10514446942737621No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT15550.10289468216461296No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT15530.10276234173739159No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA15410.10196829917406339No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG15390.10183595874684202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG553.421519E-936.0051731
CGTCAGA757.002927E-826.4046672
ACCTTAA609.744208E-625.6703551
TCTAGAC1300.025.3907873
GGTATCA29400.024.5477451
CTCGTGA450.001395857724.45200710
TATACAG450.001396126924.4511955
AGTGCGC450.001396935124.44876718
GACATAT2600.023.6957151
GTTACGT852.3637585E-723.2835939
TCCGTAA952.6870111E-823.15738928
ACATATA2750.022.4039612
TAGTACT602.867876E-422.0060774
TAGTGCA803.6172187E-621.9995228
TAAATTC3350.021.6769116
CTGAGTT2750.021.59309635
GTATGGT1950.021.4389781
GTATCAA60900.021.1360441
ACCCTTG6700.021.0186427
GGTCGGT4300.020.98323411
Produced by FastQC (version 0.11.5)