##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077095_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1511254 Sequences flagged as poor quality 0 Sequence length 50 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.21639777297529 31.0 30.0 34.0 26.0 34.0 2 30.331903836151962 31.0 31.0 34.0 26.0 34.0 3 30.38761584750148 31.0 31.0 34.0 26.0 34.0 4 33.874510174993745 37.0 35.0 37.0 30.0 37.0 5 33.475724795434786 37.0 35.0 37.0 28.0 37.0 6 33.36425313018196 37.0 35.0 37.0 27.0 37.0 7 33.192782285439776 37.0 35.0 37.0 26.0 37.0 8 33.11086422269188 37.0 35.0 37.0 26.0 37.0 9 34.44809608444378 39.0 35.0 39.0 26.0 39.0 10 34.34793555550556 39.0 35.0 39.0 25.0 39.0 11 34.379383611226174 39.0 35.0 39.0 26.0 39.0 12 34.34587302994731 39.0 35.0 39.0 25.0 39.0 13 34.29436084205567 39.0 35.0 39.0 25.0 39.0 14 35.30189365917311 39.0 35.0 41.0 25.0 41.0 15 35.306639386893266 39.0 35.0 41.0 25.0 41.0 16 35.30490043367958 39.0 35.0 41.0 25.0 41.0 17 35.349016776795956 39.0 36.0 41.0 25.0 41.0 18 35.31940031258809 39.0 35.0 41.0 25.0 41.0 19 35.45248912492539 40.0 35.0 41.0 26.0 41.0 20 35.430685377838536 40.0 35.0 41.0 26.0 41.0 21 35.42148904155093 40.0 35.0 41.0 26.0 41.0 22 35.31624200829245 40.0 35.0 41.0 25.0 41.0 23 35.25978822884836 39.0 35.0 41.0 25.0 41.0 24 35.25570883517926 39.0 35.0 41.0 25.0 41.0 25 35.21056685375192 39.0 35.0 41.0 25.0 41.0 26 35.12398643775302 39.0 35.0 41.0 25.0 41.0 27 35.02408926626497 39.0 35.0 41.0 24.0 41.0 28 34.98019062315137 39.0 35.0 41.0 24.0 41.0 29 34.932311179986954 39.0 35.0 41.0 24.0 41.0 30 34.81912636790374 39.0 34.0 41.0 24.0 41.0 31 34.67647198948688 39.0 34.0 41.0 23.0 41.0 32 34.54268706650239 39.0 34.0 41.0 22.0 41.0 33 34.3950090454682 39.0 34.0 41.0 21.0 41.0 34 34.34587302994731 39.0 34.0 41.0 19.0 41.0 35 34.26614917148275 39.0 34.0 41.0 19.0 41.0 36 34.15362076791856 39.0 34.0 41.0 18.0 41.0 37 34.07616720948299 39.0 34.0 41.0 16.0 41.0 38 33.875156657980725 38.0 33.0 41.0 16.0 41.0 39 33.756121075610054 38.0 33.0 41.0 15.0 41.0 40 33.72692810076929 38.0 33.0 41.0 14.0 41.0 41 33.64067853583845 38.0 33.0 41.0 9.0 41.0 42 33.68578412364831 39.0 33.0 41.0 9.0 41.0 43 33.630047629319755 39.0 33.0 41.0 8.0 41.0 44 33.51197283845072 39.0 33.0 41.0 2.0 41.0 45 33.32551510202785 39.0 33.0 41.0 2.0 41.0 46 33.1565170381683 39.0 33.0 41.0 2.0 41.0 47 32.95270086960895 39.0 33.0 41.0 2.0 41.0 48 32.78124259720735 38.0 33.0 41.0 2.0 41.0 49 32.52597644075715 39.0 32.0 41.0 2.0 41.0 50 32.44149759074252 39.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.034296290395147366 1101 2 0.18269597583532615 1101 3 0.17420043457567758 1101 4 -0.006209315858953346 1101 5 0.2283463364534768 1101 6 0.11085625548179223 1101 7 0.2597020642554355 1101 8 0.32616324163635113 1101 9 0.5125992182518786 1101 10 0.4518978265125142 1101 11 0.4517695422732544 1101 12 0.5088936937886004 1101 13 0.393397562737853 1101 14 0.4785804265113853 1101 15 0.3359567442155793 1101 16 0.6831606371881094 1101 17 0.6344077044261951 1101 18 0.5948889889472824 1101 19 0.533127577839899 1101 20 0.6290381408109127 1101 21 0.48628823470760096 1101 22 0.15812053700362583 1101 23 0.2245667609689832 1101 24 0.4420130347027751 1101 25 0.37032559595999714 1101 26 0.21834264997929864 1101 27 0.34829252389212684 1101 28 0.5351113735512172 1101 29 0.4672933023916386 1101 30 0.28179531595319673 1101 31 0.39314225669000535 1101 32 0.34158106011165046 1101 33 0.5358597075118396 1101 34 0.4475628485397962 1101 35 0.44232596772801 1101 36 0.42222523011705704 1101 37 0.2724077003265961 1101 38 0.38485659229917957 1101 39 0.5285498713088046 1101 40 0.4174483889778884 1101 41 0.5030407866587012 1101 42 0.568702883850051 1101 43 0.5191839552462625 1101 44 0.3911659277705226 1101 45 0.38607007981271835 1101 46 0.44290767640796247 1101 47 0.3788776115017498 1101 48 0.17148418537547627 1101 49 0.43282697660790603 1101 50 0.24182075026526206 1102 1 0.17656283124949823 1102 2 0.2057857657519655 1102 3 0.24312030824907538 1102 4 0.07455344168860734 1102 5 0.24190283298814563 1102 6 0.1863958576677689 1102 7 0.27525863793325556 1102 8 0.31930596868414796 1102 9 0.20167377333820014 1102 10 0.27137769356289 1102 11 0.34390178820183337 1102 12 0.30494697524160586 1102 13 0.36405964848151484 1102 14 0.4904858715944087 1102 15 0.5716144694900294 1102 16 0.5658593113608674 1102 17 0.5579141614014915 1102 18 0.6480556680007581 1102 19 0.5450372563881771 1102 20 0.5620468457562637 1102 21 0.7890811928555621 1102 22 0.7669116568868901 1102 23 0.7181144895267124 1102 24 0.6319159378250916 1102 25 0.7227780423854924 1102 26 0.721584543598901 1102 27 0.7548670013649215 1102 28 0.752381748043554 1102 29 0.5592507532434539 1102 30 0.616633254199165 1102 31 0.6119804244838747 1102 32 0.7271881249184773 1102 33 0.798925827653818 1102 34 0.7574534463897606 1102 35 0.85463349793379 1102 36 0.7384354508311191 1102 37 0.9177439738799507 1102 38 0.927397273548408 1102 39 0.9018314372017571 1102 40 0.7137472114160062 1102 41 0.8744600982219879 1102 42 0.9029457850343832 1102 43 0.8640269830582454 1102 44 0.637407316196601 1102 45 0.7011786587889546 1102 46 0.7285005355869103 1102 47 0.5653730454742742 1102 48 0.5722004876975788 1102 49 0.88227657908552 1102 50 0.89077146238116 1103 1 0.11061341981677941 1103 2 0.18035268970247031 1103 3 0.11487036774797588 1103 4 0.11176016504150255 1103 5 0.17215239693140205 1103 6 0.09997583178746794 1103 7 0.14947335600513156 1103 8 0.32624604949216973 1103 9 0.38008462556962996 1103 10 0.42944544543428265 1103 11 0.4089878325905474 1103 12 0.2620214522151869 1103 13 0.33036140389477 1103 14 0.3356232346491126 1103 15 0.5125982337703405 1103 16 0.46021458033963825 1103 17 0.3067026624849021 1103 18 0.3819811822807253 1103 19 0.45210273372128285 1103 20 0.26065418016402475 1103 21 0.2837173150646919 1103 22 0.38039620259378637 1103 23 0.442763699192561 1103 24 0.37451494192625745 1103 25 0.3750791192580749 1103 26 0.4194926220536743 1103 27 0.5515188589045223 1103 28 0.5664929081041166 1103 29 0.32594117343690243 1103 30 0.35386193575413927 1103 31 0.4108948514829791 1103 32 0.5022767518127154 1103 33 0.5440744229963812 1103 34 0.6595157220604833 1103 35 0.5743128805630562 1103 36 0.5388821184092549 1103 37 0.5894789802717639 1103 38 0.5184133306563012 1103 39 0.5672572582981061 1103 40 0.6349828643548818 1103 41 0.36065796417936014 1103 42 0.518677248652132 1103 43 0.5830710969481245 1103 44 0.5620936752735091 1103 45 0.6325044968718387 1103 46 0.6054630211264964 1103 47 0.4966629770631954 1103 48 0.4917559008547343 1103 49 0.418997183750875 1103 50 0.5411006742934603 1104 1 0.2966324223733494 1104 2 0.27956028054969906 1104 3 0.2227079683890274 1104 4 0.1769424954621286 1104 5 0.17643623967061473 1104 6 0.16894849812343438 1104 7 -0.016548444786003813 1104 8 0.08465665225733687 1104 9 0.3309654334962602 1104 10 0.2667904295988208 1104 11 0.012694221468834144 1104 12 0.13096486428066356 1104 13 0.21065038521019375 1104 14 0.2962075569039797 1104 15 0.3382580447623127 1104 16 0.42427125730889514 1104 17 0.34429567198617406 1104 18 0.480044040804529 1104 19 0.3802860435240092 1104 20 0.4277583889632055 1104 21 0.48936847696800356 1104 22 0.6351118638038997 1104 23 0.48082732087124924 1104 24 0.5007776452752353 1104 25 0.4898372627566303 1104 26 0.4067555670683589 1104 27 0.34231139524977294 1104 28 0.27160986158983036 1104 29 0.3443357499495363 1104 30 0.3238737646548131 1104 31 0.3167635125277357 1104 32 0.4424085232223902 1104 33 0.425565373251402 1104 34 0.4428458755818099 1104 35 0.32513899572312255 1104 36 0.3099051275289568 1104 37 0.29481852059814173 1104 38 0.17649704874688865 1104 39 0.3089827514869512 1104 40 0.23772513423188713 1104 41 0.25324137064836805 1104 42 0.26957713545082385 1104 43 0.33401677303974964 1104 44 0.1985177858800924 1104 45 0.26803571626614797 1104 46 0.33149041838677107 1104 47 0.21477391434458326 1104 48 0.2627979888464935 1104 49 0.5765759476943515 1104 50 0.4436394362018561 1105 1 0.2388027859254862 1105 2 0.246662511916373 1105 3 0.10417420911673148 1105 4 0.1574972990068062 1105 5 0.0798762382267526 1105 6 0.2315837848802218 1105 7 0.12030129140858037 1105 8 0.300176953542973 1105 9 0.32436759086506584 1105 10 0.3351036992819516 1105 11 0.3861067265073075 1105 12 0.31356694223711656 1105 13 0.3885208107280249 1105 14 0.35647912307191376 1105 15 0.37660037214573805 1105 16 0.4955552707608675 1105 17 0.3331803496141532 1105 18 0.47505189179808127 1105 19 0.39847147135803596 1105 20 0.3632484894920154 1105 21 0.2986645346261767 1105 22 0.3358536473330531 1105 23 0.3426221602718016 1105 24 0.366321710104593 1105 25 0.4029715839314534 1105 26 0.42880091633229256 1105 27 0.477673954733838 1105 28 0.38886339754497357 1105 29 0.4225466859925149 1105 30 0.3320628623019246 1105 31 0.3377337870417847 1105 32 0.4034417260066405 1105 33 0.422938148051216 1105 34 0.26752386452424304 1105 35 0.2173986981674645 1105 36 0.19201328474134272 1105 37 0.20086228161510178 1105 38 0.4185500986022035 1105 39 0.520468063210501 1105 40 0.4823049406342079 1105 41 0.3667721519575906 1105 42 0.22619814001703276 1105 43 0.29513420104223087 1105 44 0.2648283001953686 1105 45 0.28728960115434177 1105 46 0.2640049622771343 1105 47 0.33809106440384085 1105 48 0.29437883603186066 1105 49 0.46551296527937325 1105 50 0.49615686781398693 1106 1 0.35871565921654636 1106 2 0.27847780968971847 1106 3 0.3536863486610997 1106 4 0.24870621635921708 1106 5 0.32055806069180903 1106 6 0.24549516075236255 1106 7 0.3498427054357762 1106 8 0.3259888692740418 1106 9 0.3149884486199781 1106 10 0.34051542380941413 1106 11 0.31622546348984315 1106 12 0.36050034592972224 1106 13 0.3838029702551111 1106 14 0.4489256097627603 1106 15 0.3733822349874174 1106 16 0.46398897170997344 1106 17 0.2901863798377988 1106 18 0.3443652549769993 1106 19 0.404992268176791 1106 20 0.35257980799165267 1106 21 0.4340633009403092 1106 22 0.3945990706965574 1106 23 0.4630398841836154 1106 24 0.4043979551124792 1106 25 0.44209750703996065 1106 26 0.2088076895544262 1106 27 0.5339859295735394 1106 28 0.4214456799152373 1106 29 0.28615249588656155 1106 30 0.2832659658768222 1106 31 0.30691676855020233 1106 32 0.07511006884589477 1106 33 0.364935723169161 1106 34 0.29902391620861124 1106 35 0.18248976259702232 1106 36 0.3543553118993401 1106 37 0.37326648421097275 1106 38 0.22392447332129706 1106 39 0.3007249773510594 1106 40 0.34543959468874874 1106 41 0.22421238798096965 1106 42 0.21796233594247383 1106 43 0.23312609487739877 1106 44 0.2889109312209541 1106 45 0.30782171599300767 1106 46 0.307023489266939 1106 47 0.38648419455193306 1106 48 0.3410078444972484 1106 49 0.3874255952826928 1106 50 0.20110480366597727 1107 1 0.01782143840599204 1107 2 -0.024894319730695003 1107 3 0.0354827829601696 1107 4 -0.026421462024345033 1107 5 -0.06443603959659328 1107 6 -0.16749933508066306 1107 7 -0.086883352404449 1107 8 -0.16365745286731226 1107 9 -0.11738587488347463 1107 10 -0.09218808650039989 1107 11 0.0458853992064121 1107 12 -0.17356649218918108 1107 13 -0.09352068710510508 1107 14 -0.07783945234592693 1107 15 -0.16761537018321349 1107 16 -0.0826364081281099 1107 17 -0.08649217547264243 1107 18 -0.0012513373763525237 1107 19 -0.03009640593830909 1107 20 -0.09556648552476332 1107 21 -0.13036530437084792 1107 22 2.89225427366091E-4 1107 23 -0.050256586175443374 1107 24 0.011709136460027025 1107 25 0.05683797549267666 1107 26 -0.00943293986711069 1107 27 -0.031043241842262148 1107 28 -0.12937269595773415 1107 29 -0.035091633212658735 1107 30 0.02672275076820796 1107 31 -0.018115518151240906 1107 32 0.023576627109541448 1107 33 -0.05182797089045721 1107 34 -0.0396247338466722 1107 35 0.02000847103974479 1107 36 0.05055771178454194 1107 37 -0.02890462452587883 1107 38 0.0947211611555474 1107 39 0.17902864855533096 1107 40 0.12508425137906443 1107 41 0.14795145938792587 1107 42 0.030229014802621634 1107 43 -0.00550757355360787 1107 44 0.06686838935605266 1107 45 0.20031443522101 1107 46 0.15163226069196156 1107 47 0.17521745546788736 1107 48 0.10179052469867855 1107 49 0.03372171396392076 1107 50 -0.08800095321831947 1108 1 0.050262540157756774 1108 2 0.11492978256781683 1108 3 0.13286884184471504 1108 4 0.10059116227472487 1108 5 0.12876254895166284 1108 6 -0.07314781088877709 1108 7 0.0012202184934508864 1108 8 0.11712897272924039 1108 9 0.16837648072907996 1108 10 0.01995048505388297 1108 11 0.031805865112431775 1108 12 0.11811995028593714 1108 13 -0.041957039777614114 1108 14 0.005703660839984082 1108 15 -0.04202660409267622 1108 16 -0.12132402056169411 1108 17 0.023804180344761505 1108 18 0.02768854464682846 1108 19 -0.06105486303574281 1108 20 0.08434636627424652 1108 21 -0.009663747648055221 1108 22 0.03310757026072508 1108 23 0.060188358695135946 1108 24 -0.06129208831770683 1108 25 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-1.5326456922270744 2216 48 -1.3157664125159023 2216 49 -1.2997724193253113 2216 50 -1.4104254125632956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45977.0 3 6699.0 4 10885.0 5 6068.0 6 4495.0 7 3377.0 8 2933.0 9 2585.0 10 2573.0 11 2714.0 12 2906.0 13 3244.0 14 3657.0 15 3874.0 16 4042.0 17 4179.0 18 4342.0 19 4841.0 20 5783.0 21 7376.0 22 9971.0 23 11899.0 24 13991.0 25 15602.0 26 16168.0 27 17621.0 28 20636.0 29 24388.0 30 28036.0 31 32973.0 32 40696.0 33 50423.0 34 62564.0 35 80950.0 36 103473.0 37 134139.0 38 206016.0 39 509158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.21986387211443 23.96405302714485 16.389077477491785 23.427005623248938 2 13.095692674827292 23.6358570741266 40.70288939610622 22.565560854939886 3 14.262554664976236 31.380955248333997 31.537117262453425 22.819372824236346 4 9.648733888226412 20.363071596559468 42.646585872328714 27.341608642885408 5 9.63545149173202 41.495015643557274 37.38657924458103 11.482953620129678 6 24.380596081328225 45.36583073366045 18.872731533055724 11.380841651955595 7 23.40537209584017 34.85905214895086 26.400923479967993 15.334652275240982 8 23.158916203920366 37.26649487299823 22.443962410150874 17.13062651293053 9 24.936155509160642 17.37860941469595 23.28566454535706 34.39957053078635 10 12.829902656829745 29.82937638807521 36.907989024483086 20.43273193061196 11 27.221640414717683 29.320317909623743 27.51324292441901 15.944798751239558 12 19.52007053581003 31.05985085277106 31.714306139713326 17.705772471705583 13 24.219109481251277 25.39096663649852 30.28879989248045 20.101123989769754 14 20.067011633357254 25.226351288725997 31.50420659867963 23.20243047923712 15 21.127644530598303 33.9668422404593 25.232310874188002 19.673202354754395 16 20.074802070464106 30.728303890528647 29.440354982103912 19.756539056903335 17 16.15522533521543 33.227244678794364 30.398206534549104 20.219323451441102 18 16.980658641642645 30.87063256980407 33.23265640123884 18.91605238731444 19 18.7189127600934 30.467861926146018 33.41522853528081 17.39799677847977 20 17.17717856882775 31.11227333814407 33.86975260011519 17.84079549291299 21 19.203265057529094 29.641187919551946 32.799462443894235 18.35608457902472 22 18.155614445420547 29.17802297322615 33.33004532813078 19.33631725322252 23 19.20934026067568 30.109974207830863 32.50435606963356 18.176329461859897 24 18.503792881338654 30.587060842993445 32.47936406431117 18.42978221135673 25 18.679527687803148 29.983298148894722 33.29983695339171 18.037337209910426 26 17.696031182807086 29.445324167734693 34.805398735316864 18.05324591414136 27 17.972424056264032 31.220607549887724 32.307002817438466 18.499965576409778 28 18.250922631361192 27.397865122052128 34.99750103434009 19.353711212246587 29 17.671224534107218 29.67435166895266 34.376969353548944 18.277454443391182 30 18.204180413381135 30.43661595166099 32.4032346041211 18.955969030836773 31 19.407332094195645 28.56073363305638 34.253485736677945 17.778448536070027 32 17.36090288768114 30.476695413083295 33.84604285023071 18.316358849004864 33 18.556370096209342 30.229959917186893 33.065540959128235 18.148129027475523 34 18.967670540924594 27.861432127462006 34.66149275305725 18.509404578556143 35 17.901072376654845 31.294363850919595 32.75557052780945 18.04899324461611 36 18.556402860747063 29.196311685354885 32.1463412697047 20.100944184193352 37 19.35661716040558 28.30295449130331 33.19533793979827 19.145090408492837 38 17.703868909743086 29.835210652406985 34.66406798056942 17.79685245728051 39 18.174346023728972 30.568328012579215 31.74882069852766 19.50850526516415 40 17.12293412357339 29.468824738502096 35.21972706011904 18.188514077805472 41 18.092031469926688 29.249574994524064 33.701660383401595 18.95673315214766 42 18.611182432521858 29.492918004494573 32.3497375519139 19.546162011069672 43 17.708016937947793 29.006665726993052 33.34325946307579 19.942057871983366 44 17.338299025456454 29.939338353288086 33.29720236452706 19.4251602567284 45 18.667065464889983 29.29598658555711 32.36631381405724 19.670634135495664 46 18.393944386984323 28.696334617749024 32.17709452068664 20.73262647458002 47 18.648405959941055 30.129636724692517 32.653751299444224 18.568206015922204 48 18.167085974132192 30.315201869745145 31.140626592254478 20.37708556386818 49 18.4738756515289 29.62932083509512 32.411515477554055 19.48528803582193 50 18.08451013646527 28.980358344576956 32.028742207491 20.906389311466775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7088.0 1 4303.0 2 1518.0 3 2102.0 4 2686.0 5 2654.0 6 2622.0 7 2063.5 8 1505.0 9 1853.5 10 2202.0 11 3320.5 12 4439.0 13 7296.5 14 10154.0 15 13079.5 16 16005.0 17 17604.0 18 19203.0 19 18425.0 20 17647.0 21 17724.5 22 17802.0 23 20651.0 24 23500.0 25 31225.5 26 38951.0 27 50535.5 28 62120.0 29 69645.5 30 77171.0 31 84376.5 32 91582.0 33 107303.5 34 123025.0 35 129109.5 36 135194.0 37 143691.0 38 152188.0 39 153255.0 40 154322.0 41 150609.0 42 146896.0 43 118453.0 44 90010.0 45 81508.0 46 73006.0 47 67326.0 48 61646.0 49 53072.5 50 44499.0 51 38432.0 52 32365.0 53 28810.0 54 25255.0 55 21711.5 56 18168.0 57 15995.0 58 13822.0 59 12414.5 60 11007.0 61 9644.5 62 8282.0 63 7537.0 64 6792.0 65 6005.0 66 5218.0 67 4467.0 68 3716.0 69 3183.0 70 2650.0 71 2238.0 72 1826.0 73 1763.5 74 1701.0 75 1468.0 76 1235.0 77 1033.5 78 832.0 79 680.5 80 529.0 81 479.0 82 429.0 83 330.0 84 231.0 85 172.0 86 113.0 87 90.0 88 67.0 89 45.0 90 23.0 91 16.0 92 9.0 93 5.5 94 2.0 95 1.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00185276598109914 2 0.011712127809090993 3 1.9851064083205072E-4 4 0.006087659652182889 5 0.04817191550857764 6 0.0322248940284029 7 0.02957808548397556 8 0.034871702572830246 9 0.03652595791309734 10 0.04095936222501313 11 0.042084255856394755 12 0.03520255364088366 13 0.05498744751047805 14 0.050951064480226356 15 0.051480426189111826 16 0.05743574541407334 17 0.05836212840462291 18 0.053730213451875064 19 0.05207595811160797 20 0.04923063892634858 21 0.04724553251802807 22 0.04717936230441739 23 0.04751021337247081 24 0.04373851119666185 25 0.04254744735166954 26 0.04486340482804347 27 0.04380468141027253 28 0.04162106436111997 29 0.04009914944807425 30 0.03904042603030331 31 0.034805532359219564 32 0.030239787620082396 33 0.02527702159928113 34 0.022564042841243102 35 0.020578936432922593 36 0.021174468355418745 37 0.022100851345968314 38 0.015483829984899958 39 0.012572340586029879 40 0.010057872468823904 41 0.006418510720236307 42 0.006484680933846991 43 0.006021489438572205 44 0.005624468156908104 45 0.003110000039702128 46 0.004036383030251698 47 0.002051276621931191 48 0.0021174468355418747 49 6.617021361068358E-5 50 0.0017865957674884567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1511254.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.696818922750925 #Duplication Level Percentage of deduplicated Percentage of total 1 81.13758337043623 17.604274542179617 2 7.186692373811736 3.1185672617621645 3 2.8583557634478405 1.8605168224898783 4 1.607660895332318 1.3952450934085172 5 1.0310867758095512 1.1185651534191456 6 0.7532800615157058 0.9806268655694969 7 0.5345634395922843 0.8118828306089686 8 0.4472119092841288 0.7762460652668365 9 0.35774578631496823 0.6985750991447711 >10 2.7845940358175043 12.637689974574442 >50 0.5657637137896382 8.766829687167006 >100 0.6359835726807258 29.584002744503817 >500 0.07175988979883266 10.601302953547982 >1k 0.02710244764934452 9.181394309560682 >5k 6.159647193032846E-4 0.8642805967966367 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7900 0.5227446875244003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5034 0.33310085531618117 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4558 0.3016038336374957 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4183 0.2767900035334894 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 3016 0.19956936424982166 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2903 0.19209213011181442 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 2850 0.18858510879044818 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2769 0.18322532148798282 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2633 0.17422617243692987 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2617 0.17316744901915893 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2532 0.1675429808622508 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2471 0.1635065978319991 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 2463 0.16297723612311366 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2398 0.15867617223841923 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 2139 0.14153808691325218 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 2029 0.134259363416077 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2000 0.13234042722136716 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1935 0.12803936333667273 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1909 0.12631893778279496 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1893 0.12526021436502402 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1848 0.12228255475254327 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1825 0.12076063983949754 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1723 0.11401127805120781 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1690 0.11182766100205524 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1673 0.11070276737067362 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1649 0.10911468224401723 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1638 0.1083868098942997 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1637 0.10832063968068903 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1621 0.10726191626291809 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1589 0.10514446942737621 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1555 0.10289468216461296 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1553 0.10276234173739159 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1541 0.10196829917406339 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1539 0.10183595874684202 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 6.617021361068358E-5 0.0 9 0.0 0.0 0.0 6.617021361068358E-5 0.0 10 0.0 0.0 0.0 6.617021361068358E-5 0.0 11 0.0 0.0 0.0 6.617021361068358E-5 0.0 12 0.0 0.0 0.0 6.617021361068358E-5 0.0 13 0.0 0.0 0.0 1.3234042722136717E-4 0.0 14 0.0 0.0 0.0 1.3234042722136717E-4 0.0 15 0.0 0.0 0.0 1.3234042722136717E-4 0.0 16 0.0 0.0 0.0 1.9851064083205072E-4 0.0 17 0.0 0.0 0.0 1.9851064083205072E-4 0.0 18 0.0 0.0 0.0 1.9851064083205072E-4 0.0 19 0.0 0.0 0.0 3.308510680534179E-4 0.0 20 0.0 0.0 0.0 5.293617088854687E-4 0.0 21 0.0 0.0 0.0 0.0011910638449923044 0.0 22 0.0 0.0 0.0 0.0019189361947098238 0.0 23 0.0 0.0 0.0 0.002845319185259394 0.0 24 0.0 0.0 0.0 0.0044995745255264835 0.0 25 0.0 0.0 0.0 0.005293617088854686 0.0 26 0.0 0.0 0.0 0.009396170332717068 0.0 27 0.0 0.0 0.0 0.011381276741037576 0.0 28 0.0 0.0 0.0 0.01607936190739611 0.0 29 0.0 0.0 0.0 0.023225744977349937 0.0 30 0.0 0.0 0.0 0.03513638342727298 0.0 31 0.0 0.0 0.0 0.055715319860195574 0.0 32 0.0 0.0 0.0 0.07523553287534723 0.0 33 0.0 0.0 0.0 0.09899063956158263 0.0 34 0.0 0.0 0.0 0.1314140442308176 0.0 35 0.0 0.0 0.0 0.17323361923276961 0.0 36 0.0 0.0 0.0 0.2188910666241413 0.0 37 0.0 0.0 0.0 0.2799661737868022 0.0 38 0.0 0.0 0.0 0.34752596188331014 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 55 3.421519E-9 36.005173 1 CGTCAGA 75 7.002927E-8 26.404667 2 ACCTTAA 60 9.744208E-6 25.670355 1 TCTAGAC 130 0.0 25.390787 3 GGTATCA 2940 0.0 24.547745 1 CTCGTGA 45 0.0013958577 24.452007 10 TATACAG 45 0.0013961269 24.451195 5 AGTGCGC 45 0.0013969351 24.448767 18 GACATAT 260 0.0 23.695715 1 GTTACGT 85 2.3637585E-7 23.28359 39 TCCGTAA 95 2.6870111E-8 23.157389 28 ACATATA 275 0.0 22.403961 2 TAGTACT 60 2.867876E-4 22.006077 4 TAGTGCA 80 3.6172187E-6 21.99952 28 TAAATTC 335 0.0 21.67691 16 CTGAGTT 275 0.0 21.593096 35 GTATGGT 195 0.0 21.438978 1 GTATCAA 6090 0.0 21.136044 1 ACCCTTG 670 0.0 21.018642 7 GGTCGGT 430 0.0 20.983234 11 >>END_MODULE