FastQCFastQC Report
Mon 6 Feb 2017
SRR2077094_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077094_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3958162
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54850.019.5772951
TGCGATA3500.016.34054813
GGTATCA30450.015.8258251
TATCAAC68300.015.3676412
TCAACGC67250.015.3456254
CAACGCA67900.015.2307455
ATCAACG68050.015.2302773
TATACCG1756.4083E-815.088165
GCGATAC4000.014.84940314
CGATACT4050.014.66774615
GCACCGT6150.014.665156
GTACATG104200.014.6344031
AACGCAG70850.014.4995586
TACATGG106050.013.9848812
CGCACGA3950.013.92137935
ATACCGT3200.013.7485796
ATTCGGT4850.013.15743626
GTCTAGT4750.012.9710141
ACATGGG113200.012.8684613
GTGTAGG9400.012.6408251
Produced by FastQC (version 0.11.5)