FastQCFastQC Report
Mon 6 Feb 2017
SRR2077094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3958162
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61050.019.8574471
GGTATCA34300.017.3833181
TATCAAC83650.014.4661922
TCAACGC84400.014.0773134
ATCAACG84750.014.0707443
CAACGCA84800.014.0109125
CGCGTAA1901.7523962E-713.89707910
GTACATG101550.013.8878681
TACACCG3850.013.7157315
TCTAGCG1801.4477973E-613.44450228
AACGCAG88250.013.4373956
TACATGG104650.013.203162
CGTGCCG5000.013.20222610
ACCGTAT2003.2768003E-713.2010588
CTATACT7600.013.0276894
TATACTG9000.012.9569225
GCACCGT8000.012.6505356
CGCACGA4200.012.56925935
ACATGGG108250.012.3982223
GTACCGA4450.012.3600726
Produced by FastQC (version 0.11.5)