FastQCFastQC Report
Mon 6 Feb 2017
SRR2077093_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077093_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067432
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53450.019.5575311
ATACCGT2150.018.4166586
GGTATCA29700.016.8945471
GCACCGT3050.015.8671766
ACCGTGC3650.015.6693218
TATCAAC67550.015.3123072
TCAACGC67700.015.3111374
GTCTACG1158.200632E-515.3094741
ATCAACG67850.015.2772883
CAACGCA68050.015.1669645
GCGGTAT1901.07302185E-815.0579224
TATACCG1057.146873E-414.67115
AACGCAG70150.014.6128536
GTACATG100200.014.6056891
CGTCGTA1707.62162E-714.23193210
TACATGG101250.014.1281872
TACGCAA950.00625249513.89145610
TAAGGTG5900.013.8007815
GACCGTG2907.2759576E-1213.6537297
ACATGGG103200.013.56290153
Produced by FastQC (version 0.11.5)