##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077093_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3067432 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.137176309042875 31.0 30.0 34.0 23.0 34.0 2 29.155077602372277 31.0 30.0 34.0 22.0 34.0 3 29.269188363425823 31.0 30.0 34.0 22.0 34.0 4 32.64541870854839 37.0 33.0 37.0 24.0 37.0 5 32.62731300970975 37.0 33.0 37.0 25.0 37.0 6 32.6793389388909 37.0 33.0 37.0 25.0 37.0 7 32.54583377887432 37.0 33.0 37.0 23.0 37.0 8 32.458669010429574 37.0 33.0 37.0 22.0 37.0 9 33.80405205396566 38.0 34.0 39.0 22.0 39.0 10 33.75838290791776 38.0 34.0 39.0 22.0 39.0 11 33.77690328587561 38.0 34.0 39.0 22.0 39.0 12 33.6816571646902 38.0 34.0 39.0 19.0 39.0 13 33.62866756296472 38.0 34.0 39.0 19.0 39.0 14 34.575084631052945 39.0 34.0 41.0 18.0 41.0 15 34.62664372021939 39.0 34.0 41.0 18.0 41.0 16 34.597386999940014 39.0 34.0 41.0 18.0 41.0 17 34.45467739790157 39.0 34.0 41.0 17.0 41.0 18 34.42875375884453 39.0 34.0 41.0 17.0 41.0 19 34.562449632135284 39.0 34.0 41.0 18.0 41.0 20 34.545304345785006 39.0 34.0 41.0 18.0 41.0 21 34.50594308203083 39.0 34.0 41.0 18.0 41.0 22 34.44330469265496 39.0 34.0 41.0 18.0 41.0 23 34.408798630254886 39.0 34.0 41.0 18.0 41.0 24 34.412408164223365 39.0 34.0 41.0 18.0 41.0 25 34.32363912223645 39.0 34.0 41.0 18.0 41.0 26 34.27960000417287 39.0 34.0 41.0 18.0 41.0 27 34.222255293678884 39.0 34.0 41.0 17.0 41.0 28 34.12198607825699 39.0 34.0 41.0 16.0 41.0 29 34.10936640160238 39.0 34.0 41.0 16.0 41.0 30 33.95671525888756 39.0 33.0 41.0 16.0 41.0 31 33.95202697239906 39.0 33.0 41.0 16.0 41.0 32 33.81857690732834 39.0 33.0 41.0 15.0 41.0 33 33.77780371333415 39.0 33.0 41.0 15.0 41.0 34 33.70152035970153 38.0 33.0 41.0 10.0 41.0 35 33.63377313661721 38.0 33.0 41.0 9.0 41.0 36 33.54837368847949 38.0 33.0 41.0 9.0 41.0 37 33.457909417388876 38.0 33.0 40.0 9.0 41.0 38 33.35987692636707 38.0 33.0 40.0 8.0 41.0 39 33.25777947155797 38.0 33.0 40.0 8.0 41.0 40 33.1973765025598 38.0 33.0 40.0 6.0 41.0 41 33.09525850939809 38.0 33.0 40.0 2.0 41.0 42 32.9482495455482 38.0 32.0 40.0 2.0 41.0 43 32.86038060501423 38.0 32.0 40.0 2.0 41.0 44 32.725825054964545 38.0 32.0 40.0 2.0 41.0 45 32.58803878944994 38.0 32.0 40.0 2.0 41.0 46 32.4510046188473 38.0 32.0 40.0 2.0 41.0 47 32.29590941217279 38.0 31.0 40.0 2.0 41.0 48 32.127749205198356 38.0 31.0 40.0 2.0 41.0 49 31.965916440853455 38.0 31.0 40.0 2.0 41.0 50 31.76495126868338 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.728361244315959 1101 2 0.8002425157637632 1101 3 0.8188011577759156 1101 4 0.8958983068082347 1101 5 0.878467533137318 1101 6 0.9575032706825866 1101 7 0.9337394862552983 1101 8 0.9102594562738275 1101 9 1.1087669073911641 1101 10 1.1093094097765075 1101 11 1.1375514104145523 1101 12 1.1809656173784617 1101 13 1.1329113216011208 1101 14 1.4330193774243654 1101 15 1.3129704868428647 1101 16 1.3637123429246216 1101 17 1.5166202282855963 1101 18 1.3892869321886323 1101 19 1.4119111515927187 1101 20 1.4101876164486526 1101 21 1.4451402303989056 1101 22 1.4938407819843604 1101 23 1.5893640062370196 1101 24 1.4685983118856427 1101 25 1.3825561339508994 1101 26 1.4934532392765263 1101 27 1.4681256271138707 1101 28 1.586108360944408 1101 29 1.597058106783031 1101 30 1.6484370609900836 1101 31 1.4681748730449868 1101 32 1.7244779815695495 1101 33 1.5958201353887418 1101 34 1.6839682716025237 1101 35 1.6133887781442624 1101 36 1.6978555884601008 1101 37 1.8020395395454187 1101 38 1.7681364021565074 1101 39 1.6040504146075492 1101 40 1.593587903285595 1101 41 1.6896872894089867 1101 42 1.7006237558606827 1101 43 1.605608601720668 1101 44 1.6645838714258687 1101 45 1.630006163811231 1101 46 1.4829820871379198 1101 47 1.530422561256941 1101 48 1.4946584528843374 1101 49 1.594888414794319 1101 50 1.4221498649699988 1102 1 0.9549702763334835 1102 2 0.9606878249868593 1102 3 0.8751743693152712 1102 4 1.0723593684664294 1102 5 1.0559294336825928 1102 6 1.0667426797332453 1102 7 1.1103007483143017 1102 8 1.1397731347936642 1102 9 1.2858128597610161 1102 10 1.3145466283998033 1102 11 1.1442904699289258 1102 12 1.171931269613161 1102 13 1.1794579031362815 1102 14 1.5380969744789397 1102 15 1.3588972240381878 1102 16 1.4636086471610028 1102 17 1.7151277083620329 1102 18 1.7805834787701684 1102 19 1.6715315551906116 1102 20 1.5550843867334123 1102 21 1.5064372430288202 1102 22 1.4955709865795939 1102 23 1.4652762959326893 1102 24 1.5332555333052298 1102 25 1.6485532444509658 1102 26 1.7036363962882248 1102 27 1.6094373202676167 1102 28 1.6857506688159631 1102 29 1.6544030291859784 1102 30 1.6761459671745413 1102 31 1.7510685674104565 1102 32 1.88391196581712 1102 33 1.8048521529121615 1102 34 1.8409799694167575 1102 35 1.8133969339908376 1102 36 1.7291961766131507 1102 37 1.8188603905497516 1102 38 1.8053282276679923 1102 39 1.9896297127386973 1102 40 1.914845534827755 1102 41 1.9523987588595162 1102 42 1.9736675178031717 1102 43 1.7880374128312582 1102 44 1.7503950286239842 1102 45 1.7672364383137236 1102 46 1.7972478279218151 1102 47 1.7484900916665964 1102 48 1.7521024105671472 1102 49 1.7515925206805107 1102 50 1.7689842313772353 1103 1 0.4495557474872065 1103 2 0.5293651373282451 1103 3 0.526782130291771 1103 4 0.552716488626416 1103 5 0.5686366663297093 1103 6 0.5648499937269804 1103 7 0.5998071395322526 1103 8 0.6477330292125174 1103 9 0.7522658503087101 1103 10 0.6481571899033582 1103 11 0.7368643068204719 1103 12 0.72731868291757 1103 13 0.6862939854488985 1103 14 0.8090236057541773 1103 15 0.8102220724665443 1103 16 0.815932216074728 1103 17 0.9327935898077939 1103 18 0.7508619850427536 1103 19 0.8425136885905999 1103 20 0.7938028384359725 1103 21 0.6611021754306208 1103 22 0.6456378221534891 1103 23 0.6513618920721171 1103 24 0.6081860497291984 1103 25 0.7808648020270113 1103 26 0.7799225838854085 1103 27 0.7133687560779265 1103 28 0.6824085723608988 1103 29 0.8159798826815461 1103 30 0.9416717332945197 1103 31 0.844284809620035 1103 32 0.9383786158190262 1103 33 0.9689173869743684 1103 34 0.9117695401014672 1103 35 0.8863803214846371 1103 36 0.8503925863459401 1103 37 0.808434888382628 1103 38 0.8800285797463587 1103 39 0.8952237761297539 1103 40 0.8382496369008194 1103 41 0.9047507143561333 1103 42 0.8819662505752746 1103 43 0.9209891514035391 1103 44 0.794657867197536 1103 45 0.8338645147626096 1103 46 0.817337266841939 1103 47 0.7843337663309313 1103 48 0.7854618355481904 1103 49 0.8932499369930511 1103 50 0.8648559960482238 1104 1 0.8074363696916116 1104 2 0.8298943929456328 1104 3 0.8061353594914067 1104 4 0.8005498267945157 1104 5 0.8242731044359388 1104 6 0.8238110351621017 1104 7 0.7825288313490972 1104 8 0.9209811580488534 1104 9 0.9032859792541501 1104 10 0.7944066146019821 1104 11 0.9573465944655268 1104 12 0.9291733289930306 1104 13 0.9021022497857558 1104 14 1.1963608706924163 1104 15 1.023270102500419 1104 16 1.119024793810226 1104 17 1.1570826298782109 1104 18 1.1149498871962749 1104 19 1.1038822282754666 1104 20 1.0994910374298499 1104 21 1.0184998692027278 1104 22 1.069532907964863 1104 23 1.0801079970166114 1104 24 1.0728271504284805 1104 25 1.2154267005583463 1104 26 1.2166495206323447 1104 27 1.2470001276353742 1104 28 1.1551092610772855 1104 29 1.1783327235121632 1104 30 1.1957254702314444 1104 31 1.2019193781862185 1104 32 1.14930533703631 1104 33 1.2054376265421993 1104 34 1.2391823746367834 1104 35 1.2001517050525194 1104 36 1.255775805254011 1104 37 1.185166215388044 1104 38 1.1177209836899706 1104 39 1.2737951150157087 1104 40 1.3059946157050533 1104 41 1.1912309696665488 1104 42 1.2200350737218955 1104 43 1.2448595149507184 1104 44 1.3151566940806418 1104 45 1.2687372383984368 1104 46 1.4311251463514267 1104 47 1.3922254940708285 1104 48 1.3689968075938452 1104 49 1.4405605140089648 1104 50 1.4822649295747716 1105 1 0.4697485346676089 1105 2 0.5240212023467308 1105 3 0.40649780107994005 1105 4 0.47435579191048305 1105 5 0.5193965116371899 1105 6 0.4543215622836456 1105 7 0.45977536161119303 1105 8 0.5996734627113298 1105 9 0.6747971394848378 1105 10 0.5527726310341876 1105 11 0.5189554679897554 1105 12 0.5651780687837018 1105 13 0.6227337097925343 1105 14 0.7136471961525643 1105 15 0.6811763939217954 1105 16 0.5893582990511632 1105 17 0.708575908937334 1105 18 0.5164764250381779 1105 19 0.4478319090439129 1105 20 0.5928581567619844 1105 21 0.7860199754999471 1105 22 0.7498614228545861 1105 23 0.6261019916620754 1105 24 0.644112708010141 1105 25 0.6673759908024692 1105 26 0.6751254098656148 1105 27 0.71573014358151 1105 28 0.7169389190932307 1105 29 0.6923519047681737 1105 30 0.8055352630420884 1105 31 0.5604707750612334 1105 32 0.6897901491860523 1105 33 0.7114558035927914 1105 34 0.7455599502291932 1105 35 0.7426251727731739 1105 36 0.7935567231506084 1105 37 0.7541950102585133 1105 38 0.6619307876911762 1105 39 0.8484855310259647 1105 40 0.7538771030570786 1105 41 0.8540995540988874 1105 42 0.8882539805229044 1105 43 0.8213641021595848 1105 44 0.755157649531867 1105 45 0.8333866636695717 1105 46 0.9304990517595471 1105 47 0.7173241548398792 1105 48 0.804246192839102 1105 49 0.9038225349331057 1105 50 0.8558343346194697 1106 1 0.5543749592831624 1106 2 0.513425779528422 1106 3 0.5259021317373538 1106 4 0.5363723676311309 1106 5 0.6039992365760014 1106 6 0.42644085764342776 1106 7 0.5019267253727691 1106 8 0.5576009390804302 1106 9 0.5932878773772501 1106 10 0.5674340370871818 1106 11 0.5423268029806394 1106 12 0.6477863289666104 1106 13 0.6186497433860509 1106 14 0.7323535058282644 1106 15 0.7228492385820857 1106 16 0.6797131379951864 1106 17 0.7757635397544931 1106 18 0.49270076641641936 1106 19 0.687507454514602 1106 20 0.5953694888398289 1106 21 0.7502982506732039 1106 22 0.7358403844490766 1106 23 0.5776317462489473 1106 24 0.6364923687331938 1106 25 0.6928324209713708 1106 26 0.753679834386844 1106 27 0.8319697932836192 1106 28 0.8475772538086517 1106 29 0.6810076198023083 1106 30 0.7355767946414389 1106 31 0.6763640998388638 1106 32 0.7885656380990724 1106 33 0.7196970982191928 1106 34 0.8328949263431298 1106 35 0.7612669335805506 1106 36 0.7561044455461712 1106 37 0.7072820360669851 1106 38 0.7256150346351404 1106 39 0.7464425831625121 1106 40 0.8284666550248119 1106 41 0.8368498813390204 1106 42 0.7957787946203752 1106 43 0.7240989614900997 1106 44 0.8474252548486447 1106 45 0.8322075154311932 1106 46 0.8054527689109321 1106 47 0.8230284050255747 1106 48 0.8989617723039345 1106 49 0.859264284402748 1106 50 0.9531973704361114 1107 1 0.31749930264006565 1107 2 0.2450191861218265 1107 3 0.22155125006089094 1107 4 0.16922333449372218 1107 5 0.24630621899925842 1107 6 0.32272567747475733 1107 7 0.3733343552106305 1107 8 0.3951016489648609 1107 9 0.35389426065805196 1107 10 0.2791460653689768 1107 11 0.2146001368818773 1107 12 0.30891873661065006 1107 13 0.3084973311987582 1107 14 0.2811875206506471 1107 15 0.25732264631130874 1107 16 0.26233268253333364 1107 17 0.3263147650150202 1107 18 0.46762303407124506 1107 19 0.43743717595594944 1107 20 0.49409773088202513 1107 21 0.33575023409030536 1107 22 0.15554251694190668 1107 23 0.1179077493156484 1107 24 0.16931813469849288 1107 25 0.21280714908563425 1107 26 0.49745379298638426 1107 27 0.47750639492486613 1107 28 0.2387061830189765 1107 29 0.4603379148302764 1107 30 0.40021354677819687 1107 31 0.3665137434768795 1107 32 0.2892528062280988 1107 33 0.43467211028723085 1107 34 0.3579162228760566 1107 35 0.397428091544036 1107 36 0.3836944588919948 1107 37 0.37343581123239034 1107 38 0.34924105331929667 1107 39 0.40416115657875906 1107 40 0.3829659025473191 1107 41 0.45341467958986925 1107 42 0.32437329443580865 1107 43 0.20934152900010616 1107 44 0.3923893346964462 1107 45 0.3760886665797827 1107 46 0.3194577239042573 1107 47 0.3529585523975811 1107 48 0.30086461752073745 1107 49 0.2873072040154341 1107 50 0.4244892891117509 1108 1 0.08100528983026223 1108 2 0.09286797476008957 1108 3 0.07420371455545904 1108 4 0.011666247331376667 1108 5 0.03879438385584422 1108 6 -0.032617541684253126 1108 7 0.02372769968292232 1108 8 0.09854428332855036 1108 9 -0.030880175432173473 1108 10 -0.014375934074074337 1108 11 0.03917546408878536 1108 12 -0.17596012721558196 1108 13 -0.03726638364390311 1108 14 -0.12683601039919523 1108 15 -0.04689623422336098 1108 16 0.007671724582898776 1108 17 -0.1410863767282322 1108 18 -0.06915293362578012 1108 19 0.036065691689728396 1108 20 -0.09751672191664085 1108 21 0.05915945157846636 1108 22 -0.15338257356212637 1108 23 -0.33310868064815224 1108 24 -0.1919948700355647 1108 25 -0.07200209624992482 1108 26 -0.13992089373040528 1108 27 -0.16616876519766066 1108 28 -0.27558301219127657 1108 29 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-0.03722055417884462 1109 15 -0.2104309869451484 1109 16 -0.10300800376388253 1109 17 -1.4003457497387686 1109 18 -0.3705142330900486 1109 19 -0.2696985026687386 1109 20 -0.29754072343635585 1109 21 -0.04188269971604086 1109 22 -0.031717543018018546 1109 23 -0.18882060629425013 1109 24 -0.08730532192010543 1109 25 -0.1638537130158042 1109 26 -0.11771823672805226 1109 27 -0.0105275170124699 1109 28 0.1477756232180596 1109 29 0.01889509442908377 1109 30 0.02260732014697453 1109 31 0.022361688568160787 1109 32 -0.07603126240162084 1109 33 -0.06371843506402541 1109 34 -0.01597133837291409 1109 35 -0.3348288114116613 1109 36 -0.32699863861543577 1109 37 0.02675410055291394 1109 38 -0.1426063499096486 1109 39 0.04571806138697809 1109 40 -0.03268481082757546 1109 41 0.006607692190094383 1109 42 0.02270807119160878 1109 43 -0.05200427334209934 1109 44 0.05400170706548124 1109 45 0.15001663593908887 1109 46 0.1165324777505532 1109 47 0.058915796217107186 1109 48 0.13116772852069403 1109 49 0.16584092606938583 1109 50 0.21307417161562014 1110 1 -0.19329869988455783 1110 2 -0.13351945015062228 1110 3 -0.47831040501659317 1110 4 -0.35692998995097014 1110 5 -0.25843631901665987 1110 6 -0.45737414228333506 1110 7 -0.270726959444211 1110 8 -0.5465017092751907 1110 9 -0.5312591610061617 1110 10 -0.5619498995579235 1110 11 -0.441451546867647 1110 12 -0.38943748188945193 1110 13 -0.5395120219364955 1110 14 -0.6085451333805665 1110 15 -0.6728223496610255 1110 16 -0.4993202730944404 1110 17 -0.8106828569646183 1110 18 -0.5628845675694549 1110 19 -0.4794653637883144 1110 20 -0.339490880854072 1110 21 -0.4727895364098984 1110 22 -0.5442715314332887 1110 23 -0.6863300959293923 1110 24 -0.5461617593785277 1110 25 -0.4534865341145249 1110 26 -0.45012416781099773 1110 27 -0.4049757660644886 1110 28 -0.49207625666634414 1110 29 -0.5505181879322976 1110 30 -0.5374122984915388 1110 31 -0.5954039810307776 1110 32 -0.5076321568439184 1110 33 -0.4430031240426828 1110 34 -0.5181318961576693 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45 -0.7291637890191645 2214 46 -0.7481487384857104 2214 47 -0.7445157537079616 2214 48 -0.7820410531908024 2214 49 -0.7707350152937522 2214 50 -0.6414997551390975 2215 1 -0.3469096972058274 2215 2 -0.38356534154036837 2215 3 -0.4495991583225276 2215 4 -0.3075392637494545 2215 5 -0.36913915008688747 2215 6 -0.3939090119997388 2215 7 -0.32926119109850305 2215 8 -0.25680195713777465 2215 9 -0.36206736671134365 2215 10 -0.38722818335963893 2215 11 -0.44087374199006035 2215 12 -0.3954116686906275 2215 13 -0.4224939617585548 2215 14 -0.4523565539371148 2215 15 -0.4251600166567968 2215 16 -0.4916590727448309 2215 17 -0.4033820295604187 2215 18 -0.32383905561900406 2215 19 -0.41508478428445983 2215 20 -0.4789724648338023 2215 21 -0.5034734521479436 2215 22 -0.4959492383362516 2215 23 -0.6488421351041396 2215 24 -0.6451779355742744 2215 25 -0.65193472177274 2215 26 -0.7279195311005324 2215 27 -0.6062387892329397 2215 28 -0.516964567481871 2215 29 -0.5217294299069692 2215 30 -0.5284245330492041 2215 31 -0.5276887530557133 2215 32 -0.5119060780375833 2215 33 -0.5457524543606382 2215 34 -0.6536903420800186 2215 35 -0.6186895691124548 2215 36 -0.5291730861842865 2215 37 -0.5579954570678112 2215 38 -0.5703948690107978 2215 39 -0.575406573425866 2215 40 -0.5929903310788163 2215 41 -0.6596139072494012 2215 42 -0.6638795180160386 2215 43 -0.7408183569607516 2215 44 -0.7394429969417118 2215 45 -0.7052647777105534 2215 46 -0.7240917444267652 2215 47 -0.702302658883049 2215 48 -0.8700943129770273 2215 49 -0.902784874887324 2215 50 -0.8811059489834889 2216 1 -0.7926023613890933 2216 2 -0.8346827752266606 2216 3 -0.8283492438728359 2216 4 -0.6779623222463158 2216 5 -0.8339999719564766 2216 6 -0.8759465759180394 2216 7 -0.8321906292040815 2216 8 -0.9231025662559702 2216 9 -0.8862042863136068 2216 10 -1.016025492680221 2216 11 -1.1590942955738797 2216 12 -1.065094594804492 2216 13 -0.9140324007342997 2216 14 -1.0709842292174798 2216 15 -1.13974971737737 2216 16 -1.1753963013059234 2216 17 -1.1538181894759418 2216 18 -1.145592690284964 2216 19 -1.148827390293512 2216 20 -1.245784420367194 2216 21 -1.3759129558583822 2216 22 -1.2388204044602915 2216 23 -1.2674037166604677 2216 24 -1.3354257870393909 2216 25 -1.4286712952629017 2216 26 -1.367545847353135 2216 27 -1.4975433581082669 2216 28 -1.5878289677986572 2216 29 -1.6553564696707497 2216 30 -1.5117493602375802 2216 31 -1.7410103537753159 2216 32 -1.6827464264962089 2216 33 -1.589160192019392 2216 34 -1.6363471924598656 2216 35 -1.6329290513131056 2216 36 -1.7438069797499267 2216 37 -1.8615082016194826 2216 38 -1.9839286344954061 2216 39 -1.9171918861489061 2216 40 -1.9866979347902358 2216 41 -1.8989476903329283 2216 42 -1.7823050012530643 2216 43 -2.1248903561003587 2216 44 -2.109715877982783 2216 45 -2.2597389121858633 2216 46 -2.42978519078428 2216 47 -2.372181715189818 2216 48 -2.5215140570105063 2216 49 -2.4922898563595695 2216 50 -2.6507534817125915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 154647.0 3 7591.0 4 14426.0 5 10957.0 6 9054.0 7 7936.0 8 7023.0 9 6430.0 10 6368.0 11 6418.0 12 6509.0 13 6805.0 14 7125.0 15 7484.0 16 8261.0 17 8969.0 18 9646.0 19 11214.0 20 13668.0 21 18846.0 22 23349.0 23 26071.0 24 27790.0 25 29100.0 26 31975.0 27 35746.0 28 41167.0 29 47092.0 30 55351.0 31 66782.0 32 80884.0 33 100924.0 34 130199.0 35 168402.0 36 223244.0 37 305446.0 38 478434.0 39 866099.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49877742633724 17.52704703596853 12.879668342226658 25.094507195467568 2 15.835084796257629 19.759928029312977 38.797212408482594 25.607774765946793 3 19.546460989216293 24.102899640869417 28.01929939350824 28.33133997640605 4 12.809142912340393 16.005317984191596 36.73981070508651 34.4457283983815 5 14.398609383009973 37.368295851349906 33.641895241013415 14.591199524626704 6 32.7222903949517 35.962908816201754 17.342229059066288 13.972571729780261 7 29.466763475245315 31.309747006044663 20.918839967069427 18.30464955164059 8 26.810921011857193 34.53466638396424 19.692756837864728 18.961655766313832 9 26.560659023208295 16.43940491148959 18.730028561847686 38.269907503454434 10 14.814896956239831 29.238120646715863 32.95153153411722 22.99545086292708 11 35.36290668467939 23.227051961509222 22.590909728403403 18.819131625407987 12 23.57234562231222 24.993378407999668 29.42443034737589 22.00984562231222 13 28.181543015827557 21.622659183970345 25.100867016012824 25.094930784189273 14 21.974282132004994 21.39542787659285 26.654954981988883 29.97533500941327 15 24.026214840224576 28.675131617272203 23.124586377167923 24.174067165335302 16 23.52861742438451 27.963860738787538 24.804888239866372 23.702633596961583 17 22.831729840877184 28.006026470020302 26.05981848830716 23.102425200795356 18 22.787171429671123 27.3826710444513 27.015489033466828 22.814668492410746 19 23.77043190972707 26.98251666586203 27.03970056903366 22.207350855377246 20 24.06779782660963 26.396537555756293 26.358401604515507 23.17726301311857 21 24.34175002830288 26.32905271970295 26.280962291427347 23.048234960566827 22 23.77668761937202 26.242855623396817 26.473230977638483 23.507225779592677 23 23.383328489410403 26.45473443722586 26.4308573221685 23.731079751195242 24 23.187867723103565 26.43188937818949 26.747567652568588 23.63267524613836 25 23.60610187497512 26.201167152926008 26.540200085223177 23.6525308868757 26 23.35423586142994 26.381459174817785 26.702794761535 23.561510202217278 27 23.35763541912371 26.572955683683347 26.41247513595498 23.656933761237962 28 22.862740542438452 26.526529988918373 26.669227144634156 23.94150232400902 29 23.23052181632919 26.883918028769997 26.486127411108058 23.39943274379276 30 23.518982840158497 26.56749336461115 26.721261466131374 23.192262329098973 31 23.529486594037447 26.283938939265443 26.828331919890402 23.358242546806707 32 23.3395675859743 26.751010646937985 26.21941394545997 23.690007821627745 33 23.13735454733454 26.544520241870405 26.348615390436475 23.969509820358578 34 23.577674637059552 26.581135250967897 26.40037443777255 23.440815674199996 35 23.79290064934239 26.471654102449772 26.435479856751293 23.29996539145655 36 23.59336158325698 26.678139732416184 26.247834529513515 23.480664154813322 37 23.664017306629663 26.21251768795223 26.583923910327545 23.53954109509056 38 23.482939191668475 26.49274530365026 26.406735932966836 23.61757957171443 39 23.74445904997374 26.585274364686658 26.08478672846654 23.58547985687306 40 23.774707354218577 26.28095625945735 26.680403439694928 23.263932946629147 41 23.383460534816983 26.65941105620663 26.445884767555743 23.51124364142064 42 23.71570531478954 26.641848117654927 26.279940839884485 23.362505727671053 43 23.361573049025047 26.044362346306514 27.013429111595304 23.58063549307313 44 23.584249729320756 26.290683416167703 26.34087321775656 23.78419363675498 45 23.515738801986437 26.527974204597744 26.444363500471045 23.511923492944774 46 23.448641531103473 26.513418057679207 26.252221071129384 23.785719340087933 47 23.546476647457997 26.461496619758634 26.199732137354122 23.792294595429247 48 23.426197514237778 26.7057862531447 26.500057880466237 23.367958352151277 49 23.77967457988797 26.226097130383913 26.26565128043925 23.728577009288866 50 23.67200704093309 26.42244489814419 26.2371606178621 23.668387443060627 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2302.0 1 1733.0 2 1164.0 3 1095.5 4 1027.0 5 1010.5 6 994.0 7 969.5 8 945.0 9 1059.0 10 1173.0 11 1395.0 12 1617.0 13 2487.5 14 3358.0 15 4626.5 16 5895.0 17 6731.0 18 7567.0 19 8494.5 20 9422.0 21 11129.5 22 12837.0 23 16735.5 24 20634.0 25 26999.5 26 33365.0 27 41151.5 28 48938.0 29 57920.0 30 66902.0 31 74927.0 32 82952.0 33 93236.5 34 103521.0 35 117396.5 36 131272.0 37 137466.5 38 143661.0 39 153040.5 40 162420.0 41 172430.0 42 182440.0 43 184986.0 44 187532.0 45 195989.5 46 204447.0 47 216942.0 48 229437.0 49 228729.0 50 228021.0 51 219584.0 52 211147.0 53 202476.5 54 193806.0 55 189374.5 56 184943.0 57 173156.5 58 161370.0 59 145002.0 60 128634.0 61 113666.5 62 98699.0 63 85174.0 64 71649.0 65 61356.0 66 51063.0 67 42417.0 68 33771.0 69 28213.0 70 22655.0 71 18288.5 72 13922.0 73 11688.5 74 9455.0 75 7167.0 76 4879.0 77 3873.5 78 2868.0 79 2386.5 80 1905.0 81 1491.5 82 1078.0 83 894.0 84 710.0 85 569.0 86 428.0 87 343.0 88 258.0 89 212.0 90 166.0 91 119.0 92 72.0 93 54.0 94 36.0 95 28.5 96 21.0 97 19.0 98 17.0 99 27.0 100 37.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08430504734905289 2 0.09294419566595119 3 0.10063792775194365 4 0.10148554230379027 5 0.09431341917278036 6 0.05509494587003069 7 0.051769688782017015 8 0.0580941973611803 9 0.05881140967428129 10 0.04420635893477019 11 0.04730341210497902 12 0.05555135370564042 13 0.04935724736522276 14 0.05046566639456066 15 0.05004185911863735 16 0.05297590949041413 17 0.052943308930727725 18 0.05379092348257435 19 0.06141945444919398 20 0.06790696582678932 21 0.07736112813584783 22 0.07934976227671876 23 0.056561971055919084 24 0.07452487944313027 25 0.08274022048410527 26 0.07863254996361778 27 0.07377506657034288 28 0.09454162309058523 29 0.08159920089508096 30 0.07912155835891391 31 0.05320411340821899 32 0.05131328094640728 33 0.054051727960065625 34 0.04896604064898587 35 0.05590995986219092 36 0.0554535520265812 37 0.05822459959992593 38 0.04847703225368973 39 0.0528781078113549 40 0.05525794866846274 41 0.04234812703264489 42 0.038370858750903035 43 0.03856646210902149 44 0.03494779998383012 45 0.027351869576896896 46 0.02546103711508519 47 0.03048152330679213 48 0.02500462927947547 49 0.02445041976480652 50 0.0258522438313221 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3067432.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.119527362290135 #Duplication Level Percentage of deduplicated Percentage of total 1 77.65185953454595 44.35437515416208 2 12.533675106631865 14.31835196406627 3 4.357360193073709 7.4667106432688275 4 1.8673100896603148 4.266394790409312 5 1.0477723534989722 2.992413080756784 6 0.6252860182710407 2.142962509793409 7 0.4049984649103884 1.61933246286941 8 0.2675678442977584 1.2226679042907835 9 0.18612023841180145 0.9567990045574971 >10 0.9244358133207903 9.533322971828714 >50 0.0772957450783243 3.0721146804285104 >100 0.05154177295672408 5.650384673890412 >500 0.003625783091561803 1.3987343251762219 >1k 0.0011510422508275305 1.0054358345017562 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 3.2600559686408696E-5 0.0 0.0 0.0 0.0 11 6.520111937281739E-5 0.0 0.0 3.2600559686408696E-5 0.0 12 6.520111937281739E-5 0.0 0.0 3.2600559686408696E-5 0.0 13 6.520111937281739E-5 0.0 0.0 3.2600559686408696E-5 3.2600559686408696E-5 14 6.520111937281739E-5 0.0 0.0 3.2600559686408696E-5 3.2600559686408696E-5 15 6.520111937281739E-5 0.0 0.0 3.2600559686408696E-5 3.2600559686408696E-5 16 6.520111937281739E-5 0.0 0.0 6.520111937281739E-5 3.2600559686408696E-5 17 6.520111937281739E-5 0.0 0.0 9.78016790592261E-5 3.2600559686408696E-5 18 6.520111937281739E-5 0.0 0.0 9.78016790592261E-5 3.2600559686408696E-5 19 6.520111937281739E-5 0.0 0.0 9.78016790592261E-5 3.2600559686408696E-5 20 6.520111937281739E-5 0.0 0.0 1.3040223874563478E-4 3.2600559686408696E-5 21 6.520111937281739E-5 0.0 0.0 1.6300279843204347E-4 6.520111937281739E-5 22 6.520111937281739E-5 0.0 0.0 1.956033581184522E-4 6.520111937281739E-5 23 6.520111937281739E-5 0.0 0.0 2.9340503717767825E-4 6.520111937281739E-5 24 6.520111937281739E-5 0.0 0.0 3.5860615655049563E-4 9.78016790592261E-5 25 6.520111937281739E-5 0.0 0.0 3.912067162369044E-4 9.78016790592261E-5 26 6.520111937281739E-5 0.0 0.0 7.498128727874001E-4 9.78016790592261E-5 27 6.520111937281739E-5 0.0 0.0 0.0010106173502786697 9.78016790592261E-5 28 6.520111937281739E-5 0.0 0.0 0.0017930307827524782 9.78016790592261E-5 29 6.520111937281739E-5 0.0 0.0 0.002901449812090374 9.78016790592261E-5 30 6.520111937281739E-5 0.0 0.0 0.005313891228884617 9.78016790592261E-5 31 6.520111937281739E-5 0.0 0.0 0.012290411001776079 1.3040223874563478E-4 32 6.520111937281739E-5 0.0 0.0 0.021646771631775374 1.3040223874563478E-4 33 6.520111937281739E-5 0.0 0.0 0.03269836136546792 1.3040223874563478E-4 34 6.520111937281739E-5 0.0 0.0 0.04632539531438676 1.6300279843204347E-4 35 6.520111937281739E-5 0.0 0.0 0.06243007179947265 1.6300279843204347E-4 36 6.520111937281739E-5 0.0 0.0 0.0847614551846626 1.6300279843204347E-4 37 6.520111937281739E-5 0.0 0.0 0.11074410125473035 1.6300279843204347E-4 38 6.520111937281739E-5 0.0 0.0 0.1409322195243448 1.6300279843204347E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5345 0.0 19.557531 1 ATACCGT 215 0.0 18.416658 6 GGTATCA 2970 0.0 16.894547 1 GCACCGT 305 0.0 15.867176 6 ACCGTGC 365 0.0 15.669321 8 TATCAAC 6755 0.0 15.312307 2 TCAACGC 6770 0.0 15.311137 4 GTCTACG 115 8.200632E-5 15.309474 1 ATCAACG 6785 0.0 15.277288 3 CAACGCA 6805 0.0 15.166964 5 GCGGTAT 190 1.07302185E-8 15.05792 24 TATACCG 105 7.146873E-4 14.6711 5 AACGCAG 7015 0.0 14.612853 6 GTACATG 10020 0.0 14.605689 1 CGTCGTA 170 7.62162E-7 14.231932 10 TACATGG 10125 0.0 14.128187 2 TACGCAA 95 0.006252495 13.891456 10 TAAGGTG 590 0.0 13.800781 5 GACCGTG 290 7.2759576E-12 13.653729 7 ACATGGG 10320 0.0 13.5629015 3 >>END_MODULE