FastQCFastQC Report
Mon 6 Feb 2017
SRR2077093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067432
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62350.020.8916991
GGTATCA33050.020.305651
GTTCGCG750.001289890317.60324316
CGTCGTA1154.269048E-617.22084410
CGTTCGC800.001986840716.5030415
ACCGTCG2201.8189894E-1116.0021678
TTAGTCG1259.478497E-615.83491239
ATACCGT2001.2769306E-915.4010796
TATCAAC86200.015.0858142
ATCAACG86400.014.8728683
TCAACGC87250.014.6780154
CAACGCA88600.014.5040375
GTACGTA1100.00101745914.0021231
AACGCAG91850.013.9410956
GTACATG98850.013.8675551
CGCGCAA1453.8352748E-513.65679821
AGGACCG4200.013.6217795
TACATGG99750.013.5439082
CTATACT7150.013.5411774
TATTCTG18500.013.4405335
Produced by FastQC (version 0.11.5)