FastQCFastQC Report
Mon 6 Feb 2017
SRR2077092_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077092_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615447
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46450.022.882461
GGTATCA22450.022.7411791
TAGCGTA1254.8649053E-717.5979867
ATCAACG59550.017.4794063
TCAACGC59850.017.3545424
TATCAAC60750.017.242332
CAACGCA61500.016.887065
AACGCAG63650.016.278246
TATACCG1201.180557E-414.6688445
GTATAGG6200.014.55236151
CGTGCCG1901.7595085E-713.89237710
GTACATG98700.013.7565421
ACGCAGA75650.013.4921767
GTCCTAT5250.013.4131861
TACATGG99050.013.2411922
TAGCCCT13950.013.0929994
CGCAGAG77950.013.0095948
GTATACG1200.001958926712.8368391
GTCCTAG9300.012.7776831
ACATGGG101450.012.6680013
Produced by FastQC (version 0.11.5)