FastQCFastQC Report
Mon 6 Feb 2017
SRR2077092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615447
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.025.0624451
GTATCAA60650.023.940811
CGCGTAA851.4296321E-418.12128310
TATCAAC82550.017.270132
ATCAACG82850.017.1016123
TCAACGC83550.017.0112324
CAACGCA85850.016.4282575
AACGCAG88350.015.9878556
TCGCGTA1057.1550685E-414.6699
CGTTACG1057.160323E-414.66757924
TCGATCG1503.265177E-614.66210241
CGATCGT1402.7677384E-514.13845542
GGACCGT2358.221832E-1014.0438166
GTACATG96200.013.8587241
TATACTG8750.013.8301985
TACATGG95400.013.6524652
GCACCGT4700.013.5756886
CTTACAC8150.013.4975863
GTGGTAT15000.013.4934271
ACGCAGA106650.013.245067
Produced by FastQC (version 0.11.5)