FastQCFastQC Report
Mon 6 Feb 2017
SRR2077091_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077091_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171074
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33850.021.389531
GGTATCA18200.019.1051181
TTAGTCG1051.7954771E-618.85613439
CGATTCG1154.27948E-617.2164739
ACCGTAT1854.2382453E-1016.6461818
TCAACGC44150.015.7519414
ATCAACG44200.015.634543
GCACCGT4000.015.3979636
CAACGCA45700.015.3125495
TATCAAC46050.015.2927062
AACGCAG47150.014.6491756
GTACATG82050.014.2422931
CGTGCCG2401.1332304E-913.74709610
TACATGG82600.013.721172
TACCGTA1453.8444334E-513.6533647
GTTACGT1150.001425839713.389955531
GTATAGC5900.013.0550951
TTAATAC7450.012.9979283
TATACTG6500.012.8649115
GTAGGAC4300.012.7952643
Produced by FastQC (version 0.11.5)