FastQCFastQC Report
Mon 6 Feb 2017
SRR2077091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171074
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.023.165631
GTATCAA39700.021.9463881
GTATACG550.00448305720.0016421
TATAACG1307.3161027E-716.9252662
TATCAAC55450.015.5547642
TCAACGC56350.015.3070534
ATCAACG56350.015.26728253
ACCGTAT1901.0755684E-815.0552928
CAACGCA57950.014.8466895
TGTACCG4300.014.328345
AACGCAG60450.014.3045816
CGTAACA1402.7568756E-514.14424220
TTATACT5200.013.9640054
CGAATCG1901.7609818E-713.89127537
GTATAGG5550.013.8750121
CGTACAC3450.013.3930383
CCAGTAC7100.013.3256683
AACGTCG1505.2763055E-513.20087622
GTACATG82500.013.1744151
ATTATAC5050.013.0709993
Produced by FastQC (version 0.11.5)