FastQCFastQC Report
Mon 6 Feb 2017
SRR2077090_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077090_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601498
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91230.5696541612914908No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57700.36028768065898303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41590.2596943611543692No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT40750.25444927186921246No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT36240.22628813773104933No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA30060.18769926656168162No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29620.18495183884088523No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT28920.1805809311032546No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28260.17645978952205998No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA27800.17358747872304553No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC27630.1725259725581924No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC27560.17208888178442933No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA26490.16540763709976533No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG26180.16347194938738607No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT25690.1604123139710446No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA25550.15953813242351847No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG25530.15941324934530046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.1527944461997455No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA24240.15135829080023827No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG23740.14823621384478783No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT23260.14523901996755537No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG23060.1439901891853752No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG22710.14180473531655988No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21950.13705917834427517No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA21930.13693429526605716No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG21140.13200141367644544No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC20840.13012816750317516No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG20140.1257572597655445No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC19440.12138635202791387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.11495487349968592No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA18250.11395580887394177No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT17750.11083373191849132No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT17630.1100844334491832No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT17630.1100844334491832No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC17270.10783653804125888No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG17120.10689991495462373No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT16960.10590085032887958No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC16690.10421492877293634No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT16290.10171726720857596No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG16230.10134261797392191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAGC353.2267525E-431.4145643
ACAAGTA300.00575809729.32025343
ACTGTAC407.020646E-427.5083643
CCTTTAG1750.026.4063761
CTCGAGT609.739653E-625.67206419
GTGCGGG450.001396445124.4503520
CATTCCG1750.023.8810359
CTTTAGG1850.023.7895282
TCGAGTA651.8104798E-523.6980320
GTATGGT1400.023.577121
ATGTATT752.060633E-623.4723341
ATTGAAC2850.023.1548048
AACTTGC2000.023.097647
GGCATTC2000.023.097647
CACTGTA500.00257718322.0053142
TGGATCG500.00258045922.000515
CTTTAAG500.00258045922.000515
ACCTGTG500.002582332621.9977517
CAGTTTA1105.2714313E-921.99500313
TATCTGC4000.021.45518126
Produced by FastQC (version 0.11.5)