##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077090_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1601498 Sequences flagged as poor quality 0 Sequence length 50 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.98937432328982 31.0 28.0 33.0 12.0 34.0 2 28.13804075933907 31.0 28.0 34.0 15.0 34.0 3 28.245159219680573 31.0 28.0 34.0 15.0 34.0 4 31.471836992615664 35.0 32.0 37.0 12.0 37.0 5 31.647024847986074 35.0 32.0 37.0 16.0 37.0 6 31.6565890185314 35.0 32.0 37.0 16.0 37.0 7 31.52796444328997 35.0 32.0 37.0 13.0 37.0 8 31.38672230624078 35.0 32.0 37.0 11.0 37.0 9 32.551969468585035 37.0 32.0 39.0 10.0 39.0 10 32.56500413987404 37.0 32.0 39.0 10.0 39.0 11 32.61930642436019 37.0 32.0 39.0 10.0 39.0 12 32.58480934724864 37.0 32.0 39.0 10.0 39.0 13 32.48966592527746 37.0 32.0 39.0 10.0 39.0 14 33.3029463664644 38.0 32.0 41.0 10.0 41.0 15 33.36308193953411 38.0 32.0 41.0 10.0 41.0 16 33.350883984869164 38.0 32.0 41.0 10.0 41.0 17 33.244947542862995 38.0 32.0 40.0 10.0 41.0 18 33.267790531115246 38.0 32.0 40.0 10.0 41.0 19 33.45350540556404 39.0 32.0 41.0 10.0 41.0 20 33.42876294569209 39.0 32.0 41.0 10.0 41.0 21 33.391165021748385 39.0 32.0 41.0 10.0 41.0 22 33.302027227008715 39.0 32.0 41.0 10.0 41.0 23 33.263443975577864 38.0 32.0 40.0 10.0 41.0 24 33.289101828413145 38.0 32.0 41.0 10.0 41.0 25 33.21254038406542 38.0 32.0 41.0 9.0 41.0 26 33.153154109465014 38.0 32.0 40.0 8.0 41.0 27 33.100529004719334 38.0 32.0 40.0 8.0 41.0 28 33.002759916028616 38.0 32.0 40.0 6.0 41.0 29 33.01144865619564 38.0 32.0 40.0 2.0 41.0 30 32.829613274571685 38.0 32.0 40.0 2.0 41.0 31 32.834480592545226 38.0 32.0 40.0 2.0 41.0 32 32.67010698733311 38.0 31.0 40.0 2.0 41.0 33 32.62678130100693 38.0 31.0 40.0 2.0 41.0 34 32.55138813785593 38.0 31.0 40.0 2.0 41.0 35 32.48918574984171 38.0 31.0 40.0 2.0 41.0 36 32.39389871233058 38.0 31.0 40.0 2.0 41.0 37 32.31887770075267 38.0 31.0 40.0 2.0 41.0 38 32.21917916850349 38.0 30.0 40.0 2.0 41.0 39 32.164234984995296 38.0 30.0 40.0 2.0 41.0 40 32.11954619987037 38.0 30.0 40.0 2.0 41.0 41 32.069452475120166 38.0 30.0 40.0 2.0 41.0 42 31.91387126302999 38.0 30.0 40.0 2.0 41.0 43 31.879615834674787 38.0 30.0 40.0 2.0 41.0 44 31.74633686710817 38.0 30.0 40.0 2.0 41.0 45 31.631671097934557 38.0 30.0 40.0 2.0 41.0 46 31.506461450467 38.0 30.0 40.0 2.0 41.0 47 31.350977647177828 38.0 30.0 40.0 2.0 41.0 48 31.19545887662676 38.0 30.0 40.0 2.0 41.0 49 31.089584876159694 38.0 30.0 40.0 2.0 41.0 50 30.891442886597424 38.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.462769789246348 1101 2 1.7641209114311849 1101 3 1.9283900869340975 1101 4 2.024888881788293 1101 5 2.1613116990012635 1101 6 2.125517869979422 1101 7 2.0063915171409903 1101 8 1.9832824084163327 1101 9 2.4719159919654032 1101 10 2.4997523891965443 1101 11 2.3877119591758955 1101 12 2.5389221668005106 1101 13 2.4058945927878312 1101 14 2.7585993913033064 1101 15 2.927816666126681 1101 16 2.780383105395842 1101 17 2.930339506806071 1101 18 2.8036516927243085 1101 19 2.918318918014478 1101 20 2.9008128015882875 1101 21 2.868070991373088 1101 22 2.909864712901147 1101 23 2.8450770884251355 1101 24 2.8855764035150955 1101 25 2.773421082422871 1101 26 2.9983439235672975 1101 27 2.998580533846095 1101 28 2.902796102701892 1101 29 3.045396121677115 1101 30 3.1062978874534153 1101 31 3.216038540821309 1101 32 3.231473771122907 1101 33 3.335828196373292 1101 34 3.327987319516822 1101 35 3.2986382106706884 1101 36 3.1878589015027643 1101 37 3.118020722890151 1101 38 3.0037712745787317 1101 39 2.9014354954332404 1101 40 2.884231344554614 1101 41 3.034228791295668 1101 42 3.019585859116475 1101 43 3.059559023409925 1101 44 3.1464230408968454 1101 45 3.1657793740841953 1101 46 3.172812891322657 1101 47 2.9548790041763233 1101 48 3.079142545459195 1101 49 3.1807023200390887 1101 50 3.341802717819345 1102 1 0.7362271654824291 1102 2 0.8345947964347005 1102 3 0.9265950958521962 1102 4 0.9849699791308524 1102 5 0.9599954390977921 1102 6 1.1223118359732531 1102 7 1.084396204117894 1102 8 1.2761826785512902 1102 9 1.1280566373929588 1102 10 1.2322960423963778 1102 11 1.0232645552239745 1102 12 1.1587245271452247 1102 13 1.18482573864231 1102 14 1.5063665220634093 1102 15 1.4155636028190486 1102 16 1.3701689472740881 1102 17 1.5286153521525563 1102 18 1.5732028646859249 1102 19 1.4449707061326222 1102 20 1.4501790697359382 1102 21 1.3573182690368384 1102 22 1.3486883599604838 1102 23 1.3403805797112227 1102 24 1.3147127174856905 1102 25 1.4734542263796797 1102 26 1.515407375682635 1102 27 1.355365697126068 1102 28 1.5370440108031644 1102 29 1.4142833442637013 1102 30 1.33673233415837 1102 31 1.4253608556006014 1102 32 1.6194242872957147 1102 33 1.5689402466932378 1102 34 1.6347455094353691 1102 35 1.6197804096167019 1102 36 1.719593989314184 1102 37 1.6852198444916837 1102 38 1.6166177518376585 1102 39 1.836370664047685 1102 40 1.7309778354256409 1102 41 1.616846709032437 1102 42 1.6537963088609047 1102 43 1.4207432024715771 1102 44 1.3561179325468764 1102 45 1.7306594220576486 1102 46 1.6317841176189631 1102 47 1.5537933138372004 1102 48 1.563738632331816 1102 49 1.4025708432132689 1102 50 1.432488396385839 1103 1 0.369590242618024 1103 2 0.5966445640171472 1103 3 0.3803548090046007 1103 4 0.545499359397752 1103 5 0.6263084107375825 1103 6 0.49626216939347856 1103 7 0.7301473335283148 1103 8 0.6432171137792402 1103 9 0.6842579416642565 1103 10 0.7882637779832606 1103 11 0.6807488905261181 1103 12 0.7676846897595269 1103 13 0.7052423895273066 1103 14 0.8925469054131128 1103 15 0.9619325566764232 1103 16 0.7194954228229022 1103 17 0.9869242703173668 1103 18 0.6807870515289949 1103 19 0.937522130233198 1103 20 0.9515557881739056 1103 21 0.790919407990522 1103 22 0.8869815558707685 1103 23 0.8682807118316305 1103 24 0.7868421063675299 1103 25 0.8002804995518105 1103 26 0.7712558761271211 1103 27 0.8338196358219037 1103 28 0.8594801953083149 1103 29 0.8774147687950204 1103 30 1.0193394137031717 1103 31 0.7910230283909243 1103 32 1.0187649106456078 1103 33 0.9160221977381369 1103 34 0.9477169635545337 1103 35 0.9026801741170871 1103 36 1.0652602319181526 1103 37 0.9522454791189645 1103 38 0.9563029279870676 1103 39 1.096629862160718 1103 40 0.9588207450750375 1103 41 0.8834538509612457 1103 42 0.9848466846874047 1103 43 0.9606401871704122 1103 44 1.073821770425699 1103 45 1.1559365667120503 1103 46 0.9327182438232882 1103 47 1.0376141336075797 1103 48 0.8119116721929664 1103 49 1.0083282555841677 1103 50 0.9945914874390063 1104 1 0.52602964348862 1104 2 0.4469823384334468 1104 3 0.4417424627532931 1104 4 0.3941435177098569 1104 5 0.3451767783397024 1104 6 0.397610047900411 1104 7 0.44745864510827005 1104 8 0.608324487955894 1104 9 0.6507770014531786 1104 10 0.40937698324152905 1104 11 0.5274810382604045 1104 12 0.4966317335895525 1104 13 0.4666014435962893 1104 14 0.6559784196848284 1104 15 0.5418068197433286 1104 16 0.6513161922898263 1104 17 0.5549488118245165 1104 18 0.39697182714688495 1104 19 0.5947271824818259 1104 20 0.4356238350800865 1104 21 0.4228323809272254 1104 22 0.4874096361477598 1104 23 0.575175335566243 1104 24 0.5914261538457097 1104 25 0.6457556506997619 1104 26 0.48213395406599346 1104 27 0.6617415641180315 1104 28 0.596300791957276 1104 29 0.7448708408391056 1104 30 0.5985166442918199 1104 31 0.5741512166283229 1104 32 0.6331495668496672 1104 33 0.5070918460908445 1104 34 0.6061044634613495 1104 35 0.45754733809287274 1104 36 0.5846136419555563 1104 37 0.6213863230534926 1104 38 0.5967498894744168 1104 39 0.7568512657001705 1104 40 0.6943104739270325 1104 41 0.6996183967109033 1104 42 0.5906922694593604 1104 43 0.589052240783289 1104 44 0.6387254227132324 1104 45 0.5942261499903196 1104 46 0.5772116872450539 1104 47 0.664387805094858 1104 48 0.7998799834767603 1104 49 0.7619587883853569 1104 50 0.6550151896607694 1105 1 0.6617924331188902 1105 2 0.6695492841253952 1105 3 0.5313325270621405 1105 4 0.7674971285924244 1105 5 0.7001835847263358 1105 6 0.6889940087835278 1105 7 0.6410066756282049 1105 8 0.637105497188525 1105 9 0.7435426645978325 1105 10 0.6699889162536863 1105 11 0.65447265496325 1105 12 0.7789041609676133 1105 13 0.6981827476211109 1105 14 0.7774030044094573 1105 15 0.7536379711998222 1105 16 0.6380691870257067 1105 17 0.7364731883566975 1105 18 0.5093558211916474 1105 19 0.4639279158656535 1105 20 0.7208440193367522 1105 21 0.7962128840900391 1105 22 0.7053227325067652 1105 23 0.7099685182577176 1105 24 0.9146824163236005 1105 25 0.7911917420206649 1105 26 0.8820317304043854 1105 27 0.8718278520412994 1105 28 0.8715916204193377 1105 29 0.8098010141260303 1105 30 0.8782244060473374 1105 31 0.7350850155885666 1105 32 0.8871073928249373 1105 33 0.8865808564697772 1105 34 0.8598205888810071 1105 35 0.7852500658774986 1105 36 0.9504987881612976 1105 37 0.8758880990163789 1105 38 0.9127166941716212 1105 39 0.9893170452735518 1105 40 0.9582822921371985 1105 41 0.784890517714949 1105 42 0.745995187898707 1105 43 0.734353540736322 1105 44 0.7107183667068462 1105 45 0.8021924023233851 1105 46 0.78057457513939 1105 47 0.7720450271817008 1105 48 0.7852238557242366 1105 49 0.8789517563015465 1105 50 0.9030486297988034 1106 1 0.18443600209729993 1106 2 0.14391359206954135 1106 3 0.14077117189511057 1106 4 0.14833463592640328 1106 5 0.16937123261879705 1106 6 0.1639888933353184 1106 7 0.28297862451379174 1106 8 0.3077714425651621 1106 9 0.33994492293609824 1106 10 0.2709636043691077 1106 11 0.15789034231367793 1106 12 0.23883761991142904 1106 13 0.446622437314943 1106 14 0.40653324569296245 1106 15 0.37607861030822676 1106 16 0.32218974721500615 1106 17 0.4978329936020742 1106 18 0.1069368807338833 1106 19 0.25255112748997277 1106 20 0.18233380508426933 1106 21 0.26376911024128447 1106 22 0.3695292511575232 1106 23 0.2536807923201607 1106 24 0.19865613120231984 1106 25 0.22485506712038017 1106 26 0.07159911888170001 1106 27 0.3028641164278625 1106 28 0.23423595927861385 1106 29 0.24841634551478364 1106 30 0.2553832080180385 1106 31 0.3310471166250224 1106 32 0.4036327753082247 1106 33 0.1405861695943713 1106 34 0.18840148545461233 1106 35 0.24437294123110576 1106 36 0.2669807228593015 1106 37 0.13185787046430875 1106 38 0.4402144567053341 1106 39 0.326938362643844 1106 40 0.4462463563698691 1106 41 0.3890613157863996 1106 42 0.32815165261835944 1106 43 0.3177492296874078 1106 44 0.3748537344819347 1106 45 0.2709335824150898 1106 46 0.2586724897876813 1106 47 0.3232740520692019 1106 48 0.3293581444040079 1106 49 0.3392240890390106 1106 50 0.40257841874574396 1107 1 -0.12593750027222228 1107 2 -0.1944759910678293 1107 3 -0.1485148674013672 1107 4 -0.3612894298588323 1107 5 -0.5297873336325445 1107 6 -0.4028873861555873 1107 7 -0.29762687436243596 1107 8 -0.29020936115204066 1107 9 -0.2721936555356521 1107 10 -0.43988770205945116 1107 11 -0.4137284942395567 1107 12 -0.37261300636519934 1107 13 -0.17956027685377052 1107 14 -0.5953063917020884 1107 15 -0.49892087349820713 1107 16 -0.5243104986460736 1107 17 -0.5196151136499481 1107 18 -0.38660917023238994 1107 19 -0.6651410054906677 1107 20 -0.4771881308480772 1107 21 -0.45281982149804634 1107 22 -0.6601540965909933 1107 23 -0.7636983361768586 1107 24 -0.8387340247187254 1107 25 -0.7754252833406383 1107 26 -0.4851582372589789 1107 27 -0.5284026837608735 1107 28 -0.5244728379597845 1107 29 -0.4781446811827479 1107 30 -0.5986614171068965 1107 31 -0.5569333735523898 1107 32 -0.5729589553473247 1107 33 -0.5883369588598271 1107 34 -0.5296147406121747 1107 35 -0.5417517757227586 1107 36 -0.5290180505118371 1107 37 -0.46564176437654226 1107 38 -0.6148590377874505 1107 39 -0.35542120020520684 1107 40 -0.41367798112436205 1107 41 -0.48825300938801774 1107 42 -0.33343016329707353 1107 43 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29 -1.149059240001911 1108 30 -1.134639331234137 1108 31 -1.1087154437756617 1108 32 -0.8489632513449088 1108 33 -0.9344057481024777 1108 34 -0.8568749156337319 1108 35 -0.977079429266233 1108 36 -1.163851405538285 1108 37 -0.9794471855235969 1108 38 -0.9549087112109511 1108 39 -0.8409783821099523 1108 40 -1.0238742010422968 1108 41 -0.9024150348701951 1108 42 -0.9024883244365611 1108 43 -0.9620562296176836 1108 44 -1.0181851339865027 1108 45 -0.7768561983618447 1108 46 -0.7899800113032569 1108 47 -0.8140421125827011 1108 48 -0.8087496071744908 1108 49 -0.9517173932622285 1108 50 -0.9051692382983099 1109 1 -0.4006142166236444 1109 2 -0.527593243266697 1109 3 -0.527238300186152 1109 4 -0.474611196817488 1109 5 -0.4827215252316037 1109 6 -0.4426836610280702 1109 7 -0.6067816177058738 1109 8 -0.6441523452320119 1109 9 -0.7954798368963054 1109 10 -0.81437080951574 1109 11 -0.862945529140557 1109 12 -0.8679922421650375 1109 13 -0.7253432307787229 1109 14 -0.7408193807805858 1109 15 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2216 49 -1.5117287441851985 2216 50 -1.5603188395134246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104119.0 3 4585.0 4 8967.0 5 6621.0 6 5253.0 7 4435.0 8 4141.0 9 3811.0 10 4015.0 11 4304.0 12 4715.0 13 5043.0 14 4694.0 15 4757.0 16 4675.0 17 4872.0 18 5082.0 19 6181.0 20 9002.0 21 13835.0 22 17405.0 23 18348.0 24 18198.0 25 18451.0 26 18904.0 27 20716.0 28 22743.0 29 26216.0 30 30359.0 31 36471.0 32 44534.0 33 55407.0 34 69470.0 35 90221.0 36 116904.0 37 156733.0 38 244176.0 39 383135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.995400471451674 23.71288192960222 14.211293342432402 22.080424256513705 2 13.732256161280807 25.024500122500616 40.13432567162836 21.108918044590222 3 15.74759248922894 33.080294945461844 29.91870060985481 21.25341195545441 4 10.135462868972553 20.516324050784824 43.89823283211905 25.449980248123573 5 9.82660440767183 42.78832962600227 36.94145500295941 10.443610963366485 6 24.039199270246293 46.18878628462893 18.659202519149787 11.112811925974983 7 23.94323167840281 35.47963871296649 26.64463757902451 13.932492029606195 8 22.135709537305004 38.68516546956769 22.84328499952516 16.335839993602143 9 24.94979675025336 19.81894340122487 23.239387339159034 31.991872509362736 10 13.498326413677555 31.03687387318726 36.36467564660154 19.100124066533645 11 27.10497627593014 30.76363704515851 27.155141985200178 14.976244693711168 12 19.65290507840469 32.60726678837544 30.52334771596122 17.216480417258655 13 24.31109495107006 27.147994322327847 28.812811747183652 19.728098979418437 14 19.982869416473097 26.562275481274433 31.021537554212486 22.43331754803998 15 21.572518044310943 36.229877617932296 22.87224571625278 19.325358621503984 16 20.030151532380096 31.94637950445525 29.059617064292866 18.963851898871788 17 16.733370652578653 35.12805166049816 28.81930821115029 19.319269475772895 18 17.438398777440884 34.130816574272906 30.364549784234065 18.066234864052152 19 19.03260534739444 33.15856922114689 30.48137296985897 17.327452461599695 20 17.763371252003463 33.30129877433444 30.60708405322569 18.3282459204364 21 20.091826997287185 32.08951726146322 29.717788527937117 18.100867213312476 22 18.983538305109743 30.521098479569588 30.22556165433128 20.26980156098939 23 19.731852832077195 32.77558372523256 29.84413375744046 17.64842968524978 24 20.241816447967846 32.046303575378914 29.185075570764557 18.526804405888683 25 19.133791048432556 32.381405917798766 30.3787461206083 18.10605691316037 26 19.058115067746648 31.86620279435779 31.807395917765362 17.2682862201302 27 18.18643673793824 33.00424180010298 29.714534601790703 19.09478686016807 28 19.026091521896067 29.960904906106144 32.10804121391673 18.90496235808106 29 18.311651300921945 32.01364168149911 31.19681569105854 18.47789132652041 30 18.32539781238341 33.28731793556886 29.385403849170316 19.00188040287741 31 19.956765942558683 30.698554907877718 31.113589363922056 18.231089785641544 32 17.830041920993608 33.13876411163105 30.917701155170153 18.113492812205195 33 19.29457366278387 32.540529463364166 29.89902309435924 18.26587377949273 34 19.941023590563773 29.59203818472611 31.66208516593363 18.804853058776487 35 18.71837005010809 33.994651805015806 29.785947242805555 17.501030902070553 36 19.092847632715564 31.479800343257647 28.992463201642938 20.434888822383847 37 19.806436698302416 30.44448332094145 31.252475773347243 18.496604207408886 38 18.531626242584476 32.26752612613513 32.190684552219786 17.010163079060607 39 18.2764359909584 33.086926044075916 29.366513349352307 19.270124615613376 40 17.717861248563 31.75294896786125 32.592467636327285 17.936722147248464 41 18.629473271255495 31.854414019113108 31.35847015153806 18.157642558093336 42 18.84746469969441 32.39182527129575 29.407759445689628 19.352950583320215 43 18.070713960891315 31.244909024581602 30.35770505074734 20.32667196377974 44 17.603472583567946 32.6507933434236 30.79844061058803 18.94729346242043 45 18.92235757936131 31.064838179374306 29.859615547971558 20.153188693292826 46 18.238047413114682 30.67793159100505 31.081972373578772 20.0020486223015 47 20.00203624389205 31.90556825258419 30.426043434456368 17.666352069067397 48 17.839759512479382 32.65974675859623 29.611493734545597 19.888999994378796 49 19.274439299997066 31.05755556096857 31.26666454309875 18.40134059593562 50 18.165107588568276 29.896886611498807 31.62537405070288 20.312631749230036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10114.0 1 6881.0 2 3648.0 3 3473.0 4 3298.0 5 2968.0 6 2638.0 7 2404.0 8 2170.0 9 2453.5 10 2737.0 11 3204.5 12 3672.0 13 5022.5 14 6373.0 15 8247.0 16 10121.0 17 10704.0 18 11287.0 19 10689.0 20 10091.0 21 11027.5 22 11964.0 23 16292.0 24 20620.0 25 29346.0 26 38072.0 27 52028.5 28 65985.0 29 73843.0 30 81701.0 31 91949.5 32 102198.0 33 122107.5 34 142017.0 35 145994.5 36 149972.0 37 157833.0 38 165694.0 39 167533.5 40 169373.0 41 167548.5 42 165724.0 43 133357.5 44 100991.0 45 93059.0 46 85127.0 47 83430.5 48 81734.0 49 69218.0 50 56702.0 51 44239.5 52 31777.0 53 25261.0 54 18745.0 55 15203.0 56 11661.0 57 9525.5 58 7390.0 59 6501.0 60 5612.0 61 4939.5 62 4267.0 63 3873.5 64 3480.0 65 3172.5 66 2865.0 67 2561.0 68 2257.0 69 2144.0 70 2031.0 71 1891.5 72 1752.0 73 1673.0 74 1594.0 75 1433.5 76 1273.0 77 1094.5 78 916.0 79 809.5 80 703.0 81 582.5 82 462.0 83 389.5 84 317.0 85 241.0 86 165.0 87 121.5 88 78.0 89 61.0 90 44.0 91 35.0 92 26.0 93 20.5 94 15.0 95 11.0 96 7.0 97 9.5 98 12.0 99 19.0 100 26.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08329701317141826 2 0.09403695789816784 3 0.10046843642639577 4 0.10302853952986515 5 0.09459893175014893 6 0.0598189944664308 7 0.05538564518969115 8 0.06006876062286684 9 0.06269130526544522 10 0.046456505097102835 11 0.05014055590453438 12 0.05969411138821278 13 0.05257577592978574 14 0.053200191320875834 15 0.05338751593820286 16 0.05719644982385242 17 0.05582273596345422 18 0.05750865751939746 19 0.06587582376000468 20 0.07137067920159751 21 0.08211062392834707 22 0.08485805164914349 23 0.059881436005539815 24 0.07967540390309573 25 0.08591955781399664 26 0.08398387010161736 27 0.07705285926051735 28 0.09697171023629127 29 0.0875430378308309 30 0.08173597469369304 31 0.05669691751098035 32 0.05419925594661998 33 0.05832039752781458 34 0.053574840555529885 35 0.0598189944664308 36 0.05938190369266774 37 0.06169224063970108 38 0.05007811436542537 39 0.05594761904167224 40 0.05857016368425062 41 0.044458375845614545 42 0.04133629889016408 43 0.039088403482239756 44 0.03840154655204065 45 0.029097757224798283 46 0.026350329504001877 47 0.031907626484703695 48 0.026038121808456834 49 0.026163004886674848 50 0.027786484903509087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1601498.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.218630923082753 #Duplication Level Percentage of deduplicated Percentage of total 1 85.24387750401733 20.644900077222726 2 6.09393808611748 2.9517367475159304 3 2.262515790633423 1.6438510467299292 4 1.200470748582403 1.1629503197549635 5 0.7542358064083782 0.9133279312189102 6 0.5180056231722084 0.7527232202213521 7 0.3773298747967623 0.6396889081769067 8 0.2902905748179586 0.5624352233572544 9 0.23981829459095733 0.5227263688771376 >10 1.9690277352825476 10.195034640097804 >50 0.42629367877790936 7.37451639588018 >100 0.5174915708796239 27.553748717204602 >500 0.07595483188607081 12.465403351042598 >1k 0.03022424106193129 11.668998514782992 >5k 5.256389749901094E-4 0.947958537916745 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9123 0.5696541612914908 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5770 0.36028768065898303 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4159 0.2596943611543692 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 4075 0.25444927186921246 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 3624 0.22628813773104933 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 3006 0.18769926656168162 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2962 0.18495183884088523 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2892 0.1805809311032546 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2826 0.17645978952205998 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2780 0.17358747872304553 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2763 0.1725259725581924 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2756 0.17208888178442933 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 2649 0.16540763709976533 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 2618 0.16347194938738607 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2569 0.1604123139710446 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2555 0.15953813242351847 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2553 0.15941324934530046 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2447 0.1527944461997455 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 2424 0.15135829080023827 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 2374 0.14823621384478783 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 2326 0.14523901996755537 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 2306 0.1439901891853752 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2271 0.14180473531655988 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2195 0.13705917834427517 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 2193 0.13693429526605716 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 2114 0.13200141367644544 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 2084 0.13012816750317516 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 2014 0.1257572597655445 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1944 0.12138635202791387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1841 0.11495487349968592 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1825 0.11395580887394177 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1775 0.11083373191849132 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1763 0.1100844334491832 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1763 0.1100844334491832 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1727 0.10783653804125888 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1712 0.10689991495462373 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1696 0.10590085032887958 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1669 0.10421492877293634 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1629 0.10171726720857596 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1623 0.10134261797392191 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 6.244153910900919E-5 0.0 11 0.0 0.0 0.0 6.244153910900919E-5 0.0 12 0.0 0.0 0.0 1.8732461732702757E-4 0.0 13 0.0 0.0 0.0 1.8732461732702757E-4 0.0 14 0.0 0.0 0.0 1.8732461732702757E-4 0.0 15 0.0 0.0 0.0 1.8732461732702757E-4 0.0 16 0.0 0.0 0.0 3.1220769554504593E-4 0.0 17 0.0 0.0 0.0 3.7464923465405515E-4 0.0 18 0.0 0.0 0.0 4.995323128720735E-4 0.0 19 0.0 0.0 0.0 5.619738519810827E-4 0.0 20 0.0 0.0 0.0 6.244153910900919E-4 0.0 21 0.0 0.0 0.0 0.001311272321289193 0.0 22 0.0 0.0 0.0 0.0019356877123792849 0.0 23 0.0 0.0 0.0 0.0027474277207964045 0.0 24 0.0 0.0 0.0 0.004246024659412625 0.0 25 0.0 0.0 0.0 0.004745556972284699 0.0 26 0.0 0.0 0.0 0.007305660075754075 0.0 27 0.0 0.0 0.0 0.00842960777971624 0.0 28 0.0 0.0 0.0 0.013175164752000939 0.0 29 0.0 0.0 0.0 0.019544201741119878 0.0 30 0.0 0.0 0.0 0.029597289537670355 0.0 31 0.0 0.0 0.0 0.05413681440751097 0.0 32 0.0 0.0 0.0 0.08179841623280204 0.0 33 0.0 0.0 0.0 0.11788962583780935 0.0 34 0.0 0.0 0.0 0.16365927400471308 0.0 35 0.0 0.0 0.0 0.21779608841222406 0.0 36 0.0 0.0 0.0 0.2801127444430152 0.0 37 0.0 0.0 0.0 0.3658449776396848 0.0 38 0.0 0.0 0.0 0.45950728630319865 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAAGC 35 3.2267525E-4 31.41456 43 ACAAGTA 30 0.005758097 29.320253 43 ACTGTAC 40 7.020646E-4 27.508364 3 CCTTTAG 175 0.0 26.406376 1 CTCGAGT 60 9.739653E-6 25.672064 19 GTGCGGG 45 0.0013964451 24.45035 20 CATTCCG 175 0.0 23.881035 9 CTTTAGG 185 0.0 23.789528 2 TCGAGTA 65 1.8104798E-5 23.69803 20 GTATGGT 140 0.0 23.57712 1 ATGTATT 75 2.060633E-6 23.472334 1 ATTGAAC 285 0.0 23.154804 8 AACTTGC 200 0.0 23.09764 7 GGCATTC 200 0.0 23.09764 7 CACTGTA 50 0.002577183 22.005314 2 TGGATCG 50 0.002580459 22.0005 15 CTTTAAG 50 0.002580459 22.0005 15 ACCTGTG 50 0.0025823326 21.997751 7 CAGTTTA 110 5.2714313E-9 21.995003 13 TATCTGC 400 0.0 21.455181 26 >>END_MODULE