FastQCFastQC Report
Mon 6 Feb 2017
SRR2077090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601498
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69480.43384381372939584No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60800.37964455778277584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44370.2770531090266738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41380.25838308883308003No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA36620.22866091621719167No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA35550.22197967153252768No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT35270.2202313084374754No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32270.20149884670477267No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT32100.2004373405399195No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT31140.19444295278545462No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT30810.1923823819948573No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC29550.18451474806712215No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA29440.18382789113692305No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG28710.17926965878196538No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG28660.17895745108642033No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG27680.17283818025373743No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27560.17208888178442933No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA25880.1615987032141158No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT25480.15910104164975541No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC25350.1582893016413383No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG25250.15766488625024822No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24470.1527944461997455No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG24400.15235735542598244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24300.15173294003489232No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC21560.13462395831902382No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.13368733523238868No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA20950.13081502443337426No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC20880.13037793365961117No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA20380.12725585670416073No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC20310.12681876593039768No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG20270.12656899977396163No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC19980.12475819513980035No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG19850.12394645513138325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18920.11813939199424538No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT18840.11763985968137332No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG18250.11395580887394177No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT18220.11376848425661475No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT18060.11276941963087059No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA17820.11127082269225437No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC17700.11052152422294627No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17600.10989710883185617No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA17420.108773161127894No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16890.10546375955511653No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16870.1053388764768985No Hit
CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA16640.10390272107739129No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG16540.1032783056863012No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT16330.101967033365012No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA16190.10109285181748588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCC254.4404434E-544.005349
TCAAACG301.2996781E-436.67455312
CAGCGTC250.002351619335.20427320
CGCTCCA401.818151E-533.0060710
CGTTGCG300.005739527329.33964214
GCGGATC300.005742156429.33689518
TGTGGTC300.005745663329.333237
CGTCAGA407.0307124E-427.5016212
GCGTCAG407.03328E-427.4999051
ACAGGCG407.034564E-427.49904425
GAGCCGG407.0435577E-427.49303443
GGCACAG508.33089E-526.4056816
GGTTTGC551.5926217E-424.00291318
TACAATC2150.023.53487
TCTAGAC952.6850103E-823.159263
TCACCAC6850.023.1225633
ACCACTT6900.022.63547935
CACTTAA6900.022.63477537
CAAACGC500.002577578922.00473213
TAGACCC803.6149086E-622.0012975
Produced by FastQC (version 0.11.5)