##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077090_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1601498 Sequences flagged as poor quality 0 Sequence length 50 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.58753179835379 31.0 31.0 34.0 27.0 34.0 2 30.703225355261136 31.0 31.0 34.0 27.0 34.0 3 30.765685002416486 31.0 31.0 34.0 27.0 34.0 4 34.30366444416415 37.0 35.0 37.0 30.0 37.0 5 33.93159966481382 37.0 35.0 37.0 30.0 37.0 6 33.86720807643844 37.0 35.0 37.0 29.0 37.0 7 33.709608753804254 37.0 35.0 37.0 28.0 37.0 8 33.63201265315349 37.0 35.0 37.0 28.0 37.0 9 35.01093413791338 39.0 35.0 39.0 28.0 39.0 10 34.90739919750134 39.0 35.0 39.0 27.0 39.0 11 34.932761701856634 39.0 35.0 39.0 27.0 39.0 12 34.897608364169045 39.0 35.0 39.0 27.0 39.0 13 34.875228692136986 39.0 35.0 39.0 27.0 39.0 14 35.87130111932703 40.0 36.0 41.0 27.0 41.0 15 35.89187685529423 39.0 36.0 41.0 27.0 41.0 16 35.891860620494064 40.0 36.0 41.0 27.0 41.0 17 35.94889160023928 40.0 36.0 41.0 27.0 41.0 18 35.917078260478625 40.0 36.0 41.0 27.0 41.0 19 36.04881679527542 40.0 36.0 41.0 27.0 41.0 20 36.01259008753055 40.0 36.0 41.0 27.0 41.0 21 36.00014985969386 40.0 36.0 41.0 27.0 41.0 22 35.898730438626835 40.0 36.0 41.0 27.0 41.0 23 35.838873979236936 40.0 36.0 41.0 27.0 41.0 24 35.834464982160455 40.0 36.0 41.0 27.0 41.0 25 35.78388858431294 40.0 35.0 41.0 27.0 41.0 26 35.705303409682685 39.0 35.0 41.0 27.0 41.0 27 35.607473752699036 39.0 35.0 41.0 27.0 41.0 28 35.5804952613116 39.0 35.0 41.0 27.0 41.0 29 35.55475498564469 39.0 35.0 41.0 27.0 41.0 30 35.444759219181044 39.0 35.0 41.0 27.0 41.0 31 35.32835445314324 39.0 35.0 41.0 26.0 41.0 32 35.20043047197062 39.0 35.0 41.0 25.0 41.0 33 35.05458701790449 39.0 35.0 41.0 25.0 41.0 34 34.99963409258082 39.0 35.0 41.0 25.0 41.0 35 34.93177387670793 39.0 35.0 41.0 24.0 41.0 36 34.83620522785542 39.0 35.0 41.0 24.0 41.0 37 34.7395088848066 39.0 34.0 41.0 24.0 41.0 38 34.54743995933807 39.0 34.0 41.0 23.0 41.0 39 34.4479999350608 39.0 34.0 41.0 22.0 41.0 40 34.42541732802663 39.0 34.0 41.0 22.0 41.0 41 34.34759581341969 39.0 34.0 41.0 21.0 41.0 42 34.393580884896515 39.0 34.0 41.0 21.0 41.0 43 34.35119057282619 39.0 34.0 41.0 19.0 41.0 44 34.23827129350146 39.0 34.0 41.0 18.0 41.0 45 34.062736263173605 39.0 34.0 41.0 15.0 41.0 46 33.897820665402016 39.0 33.0 41.0 9.0 41.0 47 33.71195842892092 39.0 33.0 41.0 2.0 41.0 48 33.551303841778136 39.0 33.0 41.0 2.0 41.0 49 33.32062668826311 39.0 33.0 41.0 2.0 41.0 50 33.2368738518562 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.55446263478904 1101 2 0.7950333713476709 1101 3 0.7011404133633512 1101 4 0.6968469424886194 1101 5 0.8381348886952793 1101 6 0.9400823826034639 1101 7 1.0762191498692104 1101 8 1.027244622150377 1101 9 1.1861206908633406 1101 10 1.3277424968042908 1101 11 1.4515093607700749 1101 12 1.2619485327384368 1101 13 1.2741249145318463 1101 14 1.5102658158846722 1101 15 1.3801493612958353 1101 16 1.4772758721758024 1101 17 1.5243303463465878 1101 18 1.5510607183107794 1101 19 1.4666203244715845 1101 20 1.5903533729615802 1101 21 1.4399288228764533 1101 22 1.5029989532475838 1101 23 1.6450851894023089 1101 24 1.6328405525285206 1101 25 1.6461539271728398 1101 26 1.54162588496866 1101 27 1.697306706195235 1101 28 1.5273785832928013 1101 29 1.5469590597514582 1101 30 1.5769039505561508 1101 31 1.688435314557303 1101 32 1.6825019585047514 1101 33 1.6520749018128384 1101 34 1.8037902842047941 1101 35 1.675147665991723 1101 36 1.75050790954581 1101 37 1.6600403927608696 1101 38 1.722578381414479 1101 39 1.8258628590291792 1101 40 1.9231413363878858 1101 41 1.8485270660178372 1101 42 1.9069482332435044 1101 43 1.782046925017113 1101 44 1.6944098457590968 1101 45 1.5422756484846118 1101 46 1.536239598561174 1101 47 1.4900987782116601 1101 48 1.5984452853426916 1101 49 1.9047252800979493 1101 50 1.8546758767189573 1102 1 0.42119784133007343 1102 2 0.4842132908590244 1102 3 0.4202000614487744 1102 4 0.5675467147618747 1102 5 0.6320895112404585 1102 6 0.6669902253465949 1102 7 0.6884937182870701 1102 8 0.5940618022919679 1102 9 0.8187384915427387 1102 10 0.9270454369174033 1102 11 0.8668248788642998 1102 12 0.8895127997402668 1102 13 0.884982754381987 1102 14 0.8543713253735135 1102 15 0.9815185311283443 1102 16 0.9260336761394967 1102 17 0.9891377184187746 1102 18 1.1384281550253874 1102 19 0.9500689147162902 1102 20 0.9670138734921849 1102 21 0.9350650893551986 1102 22 1.0048767206091185 1102 23 1.0788342324583482 1102 24 0.9692794735004213 1102 25 0.9488756446920092 1102 26 0.9291034893863355 1102 27 1.0720508104338151 1102 28 1.10047940279469 1102 29 0.9818392114042922 1102 30 0.8827062834759971 1102 31 0.9291537524635558 1102 32 0.989581888952614 1102 33 0.9940315725274544 1102 34 0.977020468817642 1102 35 0.8649083572589404 1102 36 0.9750583841799809 1102 37 0.861558325781985 1102 38 1.0319609974592936 1102 39 1.04149924808371 1102 40 0.9034696789525825 1102 41 1.0497162350677343 1102 42 0.9591033095030355 1102 43 0.9080357671090695 1102 44 0.9614360903548089 1102 45 0.9763962650625473 1102 46 1.094665772320461 1102 47 1.0559794994321834 1102 48 1.0830049172073117 1102 49 1.0587343109064449 1102 50 1.0819113128596456 1103 1 0.24321330513658168 1103 2 0.33450987481489847 1103 3 0.2767172218774121 1103 4 0.12724412263221296 1103 5 0.3979673544471751 1103 6 0.29872667669189923 1103 7 0.347065874671884 1103 8 0.26741822513372426 1103 9 0.45216878001677685 1103 10 0.5020495772843887 1103 11 0.5281218794562008 1103 12 0.5927834668555079 1103 13 0.5114431156596453 1103 14 0.6384471026299394 1103 15 0.6313754935174742 1103 16 0.6430316092980064 1103 17 0.5616375865954311 1103 18 0.5825692201134416 1103 19 0.5189130059484413 1103 20 0.6152132037553315 1103 21 0.4998222856100796 1103 22 0.5990750824348581 1103 23 0.6745040027292504 1103 24 0.5175884585052373 1103 25 0.5729655994561824 1103 26 0.4643541653987171 1103 27 0.5856503378007147 1103 28 0.502934584714616 1103 29 0.5805693255744444 1103 30 0.5759115884929287 1103 31 0.39797418976057486 1103 32 0.42978960540840205 1103 33 0.5032131612252613 1103 34 0.555784916470401 1103 35 0.5423711700794485 1103 36 0.6190687027914308 1103 37 0.7143877745970215 1103 38 0.7106170900371609 1103 39 0.6569352788568494 1103 40 0.6513332124568834 1103 41 0.6665390102795996 1103 42 0.8021696615760732 1103 43 0.7192565700951832 1103 44 0.8651038613668902 1103 45 0.7333736189261586 1103 46 0.6514359593016579 1103 47 0.8045580353241562 1103 48 0.7515046710132935 1103 49 0.9561423297761209 1103 50 0.9387814256484432 1104 1 0.21763556788631178 1104 2 0.303047177404256 1104 3 0.21426724590250146 1104 4 0.15762484400830346 1104 5 0.2494379337126773 1104 6 0.28700182458680246 1104 7 0.2130280878533526 1104 8 0.35008739528893784 1104 9 0.4624350145707439 1104 10 0.41818779370805714 1104 11 0.34526809904617295 1104 12 0.287158106394493 1104 13 0.4138295962792924 1104 14 0.4584055111052052 1104 15 0.3084137798138258 1104 16 0.4369417751287088 1104 17 0.5550823637991797 1104 18 0.4736291771054866 1104 19 0.597524850688778 1104 20 0.41907751521804215 1104 21 0.5618103970891895 1104 22 0.5278981378430103 1104 23 0.5088778018985067 1104 24 0.6090562659060481 1104 25 0.62383338286579 1104 26 0.5098950890328311 1104 27 0.6093536777960082 1104 28 0.6892347281874223 1104 29 0.5995179688178354 1104 30 0.6716495246126328 1104 31 0.7271234645011617 1104 32 0.7076681936685674 1104 33 0.6576585476970678 1104 34 0.6742091080042201 1104 35 0.5203880466013473 1104 36 0.5404651600908252 1104 37 0.7382224223634992 1104 38 0.7640569808975286 1104 39 0.8441886320337488 1104 40 0.8871053800889541 1104 41 0.8850000696469777 1104 42 0.8665555376716867 1104 43 0.8383579708695379 1104 44 0.8137564233763541 1104 45 0.8737839836669536 1104 46 0.7848917561924296 1104 47 0.79425960918757 1104 48 0.7288080804068713 1104 49 0.6839242077449512 1104 50 0.6700831990319003 1105 1 0.5367911700844843 1105 2 0.5761120509882645 1105 3 0.5531693710360734 1105 4 0.4219809083577388 1105 5 0.38626717548731193 1105 6 0.5246924607443191 1105 7 0.543105090515688 1105 8 0.5106150053291003 1105 9 0.6281638885810708 1105 10 0.6360984153173419 1105 11 0.5126791429302457 1105 12 0.5629519689822047 1105 13 0.5871577667347765 1105 14 0.7942056199611898 1105 15 0.7462186174215688 1105 16 0.73687071681519 1105 17 0.7348005030051681 1105 18 0.7144977218652144 1105 19 0.632744333766361 1105 20 0.7099549773259142 1105 21 0.722782307195061 1105 22 0.8495964628829071 1105 23 0.7994399808925294 1105 24 0.6995875600236161 1105 25 0.7526850214553207 1105 26 0.802707145485499 1105 27 0.7638628615097147 1105 28 0.7974956242217033 1105 29 0.802945200204789 1105 30 0.7136080811340193 1105 31 0.7362617253031587 1105 32 0.8841754276094633 1105 33 0.9243483010288642 1105 34 0.7732825782088142 1105 35 0.816994554302191 1105 36 0.7894673822510825 1105 37 0.7739185422364159 1105 38 0.8270007550789273 1105 39 0.9211210114775596 1105 40 0.8579280809351033 1105 41 0.8731521228890742 1105 42 0.8141183959020211 1105 43 0.9202332504131476 1105 44 0.8977231963967611 1105 45 1.0065493564409778 1105 46 0.9967478201294 1105 47 0.8532573124548506 1105 48 0.966626578633047 1105 49 1.0036346488294896 1105 50 0.977678750355814 1106 1 0.2512349398314164 1106 2 0.1584431587663815 1106 3 0.24904102684631724 1106 4 0.14484272594235392 1106 5 0.21762492824291257 1106 6 0.12783922259855274 1106 7 0.15294318993223754 1106 8 0.17301577470512797 1106 9 0.33833923683778977 1106 10 0.22683226797318667 1106 11 0.18171256222835552 1106 12 0.2420862884256536 1106 13 0.15442350484307354 1106 14 0.2909053078912436 1106 15 0.12242406076330781 1106 16 0.2344862159054557 1106 17 0.19650642334174506 1106 18 0.1830301822041207 1106 19 0.13631252651676107 1106 20 0.309716945305496 1106 21 0.46257203821478043 1106 22 0.4483467001182291 1106 23 0.4410508114756695 1106 24 0.4762642083220001 1106 25 0.244629748426064 1106 26 0.3623777248192823 1106 27 0.5629886365375896 1106 28 0.4944473219273462 1106 29 0.47972633458955016 1106 30 0.39276858914811186 1106 31 0.3796071542853525 1106 32 0.29140328379256175 1106 33 0.4141537688553356 1106 34 0.4220189086820696 1106 35 0.5466191758497345 1106 36 0.47516872242752584 1106 37 0.51718504265299 1106 38 0.49944988969654247 1106 39 0.6387553694939356 1106 40 0.6284824409140484 1106 41 0.5678331453098053 1106 42 0.5736113977953323 1106 43 0.557260765608433 1106 44 0.6247294341672145 1106 45 0.6763409821880018 1106 46 0.6951165403744142 1106 47 0.6740222616719009 1106 48 0.9078696806660886 1106 49 1.0725571295560457 1106 50 1.0224394349376738 1107 1 -0.011963517858681882 1107 2 -0.05383184775295291 1107 3 -0.03737344676242671 1107 4 -0.10878772076009113 1107 5 -0.10456061237234593 1107 6 -0.10613518490682594 1107 7 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-0.8887025907153614 2216 13 -1.0182346544303087 2216 14 -1.208781983726361 2216 15 -1.1320946831927898 2216 16 -1.098042270749879 2216 17 -1.0652775068117109 2216 18 -1.137774975025657 2216 19 -1.2952556650453744 2216 20 -1.360166520141739 2216 21 -1.4382247858757538 2216 22 -1.3333543817233817 2216 23 -1.1533517516116305 2216 24 -1.3215217300662587 2216 25 -1.3228203713520301 2216 26 -1.4870258001158732 2216 27 -1.3674072220819937 2216 28 -1.4442829698964346 2216 29 -1.601083118606418 2216 30 -1.5692703385166098 2216 31 -1.5162268799460819 2216 32 -1.523795119971325 2216 33 -1.376005535577704 2216 34 -1.6381927668568892 2216 35 -1.573539343577437 2216 36 -1.5450637255744653 2216 37 -1.6634745693125694 2216 38 -1.6477154196000825 2216 39 -1.6445265071359216 2216 40 -2.009089837854475 2216 41 -1.970949595257835 2216 42 -1.697956638490922 2216 43 -1.8588682583014702 2216 44 -1.7441154943929433 2216 45 -1.6881363807443677 2216 46 -1.8254346545166626 2216 47 -1.8979256829498183 2216 48 -1.8155908667504228 2216 49 -1.8134259436470472 2216 50 -2.025934179548532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33424.0 3 5276.0 4 9000.0 5 5316.0 6 4029.0 7 3058.0 8 2698.0 9 2396.0 10 2394.0 11 2405.0 12 2558.0 13 2861.0 14 3178.0 15 3410.0 16 3650.0 17 3951.0 18 4109.0 19 4729.0 20 5670.0 21 7434.0 22 9697.0 23 11988.0 24 14200.0 25 15629.0 26 16810.0 27 18646.0 28 22220.0 29 25496.0 30 29731.0 31 35324.0 32 43800.0 33 54266.0 34 67776.0 35 86432.0 36 111821.0 37 147506.0 38 226825.0 39 551785.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.78084266067219 23.878362134906883 15.8983437143883 23.442451490032624 2 13.173016997078685 24.508753326913684 40.10918396786679 22.209045708140838 3 14.955088838866185 31.71917489595659 31.46522468068898 21.860511584488243 4 9.602877491187371 19.553764061958482 43.68684802937439 27.156510417479762 5 9.33389637388558 41.72061777875505 37.80284252188862 11.142643325470756 6 24.06659529790628 45.506391334816506 18.84791083984875 11.579102527428464 7 23.28290401900171 34.29932440999076 27.430066097523042 14.98770547348448 8 22.702515999035626 36.951057878845795 23.267463401776602 17.078962720341977 9 24.957917321199364 17.135629584982027 24.38578027064081 33.5206728231778 10 13.541120122325992 29.512332538814018 37.13041110128372 19.816136237576266 11 27.271091866825493 28.82230843562239 27.94925721624274 15.957342481309384 12 19.878889329581547 30.895338269785107 31.84392401070571 17.38184838992764 13 24.63387863442962 25.012009867769635 30.216827308409034 20.13728418939171 14 19.95223721724008 24.537864155299633 32.73262336740736 22.777275260052924 15 21.30066735007112 34.5236065014627 24.74323919967966 19.43248694878652 16 19.481856545356294 30.26278881624774 30.463011841583292 19.792342796812672 17 16.08447964256018 33.247600918838245 30.66125992289705 20.006659515704523 18 16.792689509035664 31.526992043818968 33.14940078762194 18.53091765952343 19 18.39629183090849 31.00412921120197 33.2129136238982 17.386665333991342 20 16.89261195754702 31.09755564301019 33.5376023687712 18.47223003067159 21 19.12941088283246 29.960958728691274 32.68778851499497 18.221841873481296 22 17.92624787928609 28.632948460717756 33.26659903702723 20.174204622968926 23 19.11910673850238 30.415553993909583 32.782634496759584 17.682704770828455 24 19.471640646179257 30.090485583896236 32.069350210316834 18.368523559607674 25 18.259505689171835 30.497658869561306 33.14660923359835 18.096226207668515 26 18.221982637461757 29.712702337034123 34.67616704915555 17.38914797634857 27 17.40781110920249 30.87565001483517 32.7130252822587 19.00351359370364 28 18.047761581858794 27.796086328634463 35.29106431673155 18.865087772775198 29 17.54419059028847 29.966526396924852 34.17980303105069 18.309479981735993 30 17.78834081837886 30.9489247143919 32.25325896175444 19.009475505474807 31 19.27242996374829 28.477890455382415 34.15736639209308 18.09231318877621 32 17.015636827864984 30.85169371903676 33.821354425604525 18.311315027493734 33 18.333311474058352 30.568197502921336 32.934058061981666 18.164432961038653 34 19.027553463762306 27.465028872492624 34.59446732343312 18.912950340311944 35 17.96858118193412 31.83444228912457 32.80951982443233 17.387456704508978 36 18.38023273896401 29.37665852898433 31.805285184234506 20.437823547817157 37 18.974611668190516 28.411861544533878 33.87636084756009 18.737165939715513 38 17.939313624958004 30.068782388111526 34.90403314344381 17.087870843486655 39 17.650607375433705 31.01430323558873 31.889146177269197 19.445943211708368 40 17.254107778926805 29.366123125958566 35.35862196444005 18.02114713067458 41 17.97226303717807 29.7063009202567 33.678260473173175 18.64317556939205 42 18.383135340900008 29.985487770761733 31.963434677483814 19.66794221085445 43 17.424182817977567 29.247891556108396 32.81073398700402 20.517191638910013 44 16.91128259759139 30.43245315725649 33.285167807111435 19.37109643804069 45 18.450686811642893 28.98165296873224 32.15263840825048 20.415021811374384 46 17.875895986567148 28.798080735734622 32.877079291415825 20.448943986282405 47 19.49140477186585 29.929002728742965 32.464298425821276 18.115294073569906 48 17.643712647762037 30.656887360855013 31.150369315325786 20.549030676057164 49 18.88610329723165 29.375801985020246 32.80659633654804 18.931498381200065 50 17.769592309128477 28.24251022333674 32.91087073668593 21.077026730848843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4744.0 1 2936.0 2 1128.0 3 1717.0 4 2306.0 5 2408.0 6 2510.0 7 1835.5 8 1161.0 9 1542.0 10 1923.0 11 3027.5 12 4132.0 13 6679.0 14 9226.0 15 11911.5 16 14597.0 17 16090.0 18 17583.0 19 16900.5 20 16218.0 21 16582.0 22 16946.0 23 20202.0 24 23458.0 25 31343.5 26 39229.0 27 52811.5 28 66394.0 29 73167.0 30 79940.0 31 89933.0 32 99926.0 33 120049.5 34 140173.0 35 144896.5 36 149620.0 37 157173.0 38 164726.0 39 167394.0 40 170062.0 41 167146.5 42 164231.0 43 131579.0 44 98927.0 45 91369.0 46 83811.0 47 82444.5 48 81078.0 49 68228.5 50 55379.0 51 43167.0 52 30955.0 53 24305.0 54 17655.0 55 14389.5 56 11124.0 57 8733.5 58 6343.0 59 5642.0 60 4941.0 61 4426.0 62 3911.0 63 3589.0 64 3267.0 65 3030.5 66 2794.0 67 2433.5 68 2073.0 69 2017.5 70 1962.0 71 1825.0 72 1688.0 73 1629.5 74 1571.0 75 1386.0 76 1201.0 77 1049.0 78 897.0 79 802.0 80 707.0 81 569.5 82 432.0 83 361.5 84 291.0 85 217.0 86 143.0 87 107.0 88 71.0 89 45.0 90 19.0 91 17.0 92 15.0 93 9.5 94 4.0 95 4.0 96 4.0 97 3.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0014361553995072113 2 0.010240412413877508 3 1.8732461732702757E-4 4 0.006431478528227947 5 0.04158606504660012 6 0.026475212582219895 7 0.023665343322314483 8 0.028972874146580264 9 0.030159263389651438 10 0.03309401572777487 11 0.03502970344015416 12 0.030159263389651438 13 0.045644765088685715 14 0.04183583120303616 15 0.04302222044610733 16 0.04889172512235419 17 0.04876684204413618 18 0.04558232354957671 19 0.04420860968917851 20 0.04171094812481814 21 0.03921328656045777 22 0.03883863732580372 23 0.03890107886491272 24 0.03621609268322533 25 0.03509214497926316 26 0.03621609268322533 27 0.03577900190946227 28 0.03409308035351902 29 0.03340622342331992 30 0.03296913264955686 31 0.02934752338123432 32 0.025351264878257732 33 0.02166721407082619 34 0.01998129251488294 35 0.018232929419830683 36 0.019044669428247805 37 0.018108046341612664 38 0.013487372447545986 39 0.011364360117839672 40 0.009116464709915341 41 0.0060568292935738914 42 0.005619738519810827 43 0.004995323128720735 44 0.004745556972284699 45 0.0024976615643603674 46 0.0031845184945594684 47 0.0017483630950522575 48 0.001311272321289193 49 1.8732461732702757E-4 50 9.366230866351379E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1601498.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.15365917011947 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61100386778344 14.633846895769228 2 7.619240652233896 2.766341958715458 3 2.9396778389599674 1.600977286752978 4 1.6146435211290648 1.1724675265527456 5 1.0039727432362744 0.9112889498400594 6 0.6996689722249391 0.7620931232207597 7 0.5255222091645323 0.6678105750050807 8 0.40241187784955046 0.5844198461190789 9 0.31445389843629473 0.5137640007235071 >10 2.7736153658485327 10.72837142914382 >50 0.6044293953040655 7.836205814026057 >100 0.7339995935283191 29.204849604502275 >500 0.10838044163787759 13.477133703882583 >1k 0.048284876242515984 14.318871246600137 >5k 6.947464207556256E-4 0.8215580391462609 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6948 0.43384381372939584 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6080 0.37964455778277584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4437 0.2770531090266738 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4138 0.25838308883308003 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 3662 0.22866091621719167 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3555 0.22197967153252768 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 3527 0.2202313084374754 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3227 0.20149884670477267 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3210 0.2004373405399195 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 3114 0.19444295278545462 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 3081 0.1923823819948573 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2955 0.18451474806712215 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2944 0.18382789113692305 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 2871 0.17926965878196538 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2866 0.17895745108642033 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 2768 0.17283818025373743 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2756 0.17208888178442933 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 2588 0.1615987032141158 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 2548 0.15910104164975541 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2535 0.1582893016413383 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 2525 0.15766488625024822 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2447 0.1527944461997455 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2440 0.15235735542598244 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2430 0.15173294003489232 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 2156 0.13462395831902382 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2141 0.13368733523238868 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 2095 0.13081502443337426 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 2088 0.13037793365961117 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 2038 0.12725585670416073 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 2031 0.12681876593039768 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 2027 0.12656899977396163 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1998 0.12475819513980035 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1985 0.12394645513138325 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1892 0.11813939199424538 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1884 0.11763985968137332 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1825 0.11395580887394177 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1822 0.11376848425661475 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1806 0.11276941963087059 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1782 0.11127082269225437 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1770 0.11052152422294627 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1760 0.10989710883185617 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1742 0.108773161127894 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1689 0.10546375955511653 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1687 0.1053388764768985 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 1664 0.10390272107739129 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1654 0.1032783056863012 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1633 0.101967033365012 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1619 0.10109285181748588 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 6.244153910900919E-5 0.0 12 0.0 0.0 0.0 1.8732461732702757E-4 0.0 13 0.0 0.0 0.0 1.8732461732702757E-4 0.0 14 0.0 0.0 0.0 1.8732461732702757E-4 0.0 15 0.0 0.0 0.0 1.8732461732702757E-4 0.0 16 0.0 0.0 0.0 3.1220769554504593E-4 0.0 17 0.0 0.0 0.0 3.7464923465405515E-4 0.0 18 0.0 0.0 0.0 4.995323128720735E-4 0.0 19 0.0 0.0 0.0 5.619738519810827E-4 0.0 20 0.0 0.0 0.0 6.244153910900919E-4 0.0 21 0.0 0.0 0.0 0.0013737138603982023 0.0 22 0.0 0.0 0.0 0.001998129251488294 0.0 23 0.0 0.0 0.0 0.002684986181687395 0.0 24 0.0 0.0 0.0 0.004308466198521634 0.0 25 0.0 0.0 0.0 0.004807998511393708 0.0 26 0.0 0.0 0.0 0.007492984693081103 0.0 27 0.0 0.0 0.0 0.008679373936152278 0.0 28 0.0 0.0 0.0 0.01386202168220004 0.0 29 0.0 0.0 0.0 0.020293500210427987 0.0 30 0.0 0.0 0.0 0.030533912624305493 0.0 31 0.0 0.0 0.0 0.05588517750256323 0.0 32 0.0 0.0 0.0 0.0841711947189444 0.0 33 0.0 0.0 0.0 0.12107414433236882 0.0 34 0.0 0.0 0.0 0.1684048309769978 0.0 35 0.0 0.0 0.0 0.22466465771421507 0.0 36 0.0 0.0 0.0 0.2886672353009495 0.0 37 0.0 0.0 0.0 0.3788952593134678 0.0 38 0.0 0.0 0.0 0.47474302184579686 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCTCC 25 4.4404434E-5 44.00534 9 TCAAACG 30 1.2996781E-4 36.674553 12 CAGCGTC 25 0.0023516193 35.204273 20 CGCTCCA 40 1.818151E-5 33.00607 10 CGTTGCG 30 0.0057395273 29.339642 14 GCGGATC 30 0.0057421564 29.336895 18 TGTGGTC 30 0.0057456633 29.33323 7 CGTCAGA 40 7.0307124E-4 27.501621 2 GCGTCAG 40 7.03328E-4 27.499905 1 ACAGGCG 40 7.034564E-4 27.499044 25 GAGCCGG 40 7.0435577E-4 27.493034 43 GGCACAG 50 8.33089E-5 26.40568 16 GGTTTGC 55 1.5926217E-4 24.002913 18 TACAATC 215 0.0 23.5348 7 TCTAGAC 95 2.6850103E-8 23.15926 3 TCACCAC 685 0.0 23.12256 33 ACCACTT 690 0.0 22.635479 35 CACTTAA 690 0.0 22.634775 37 CAAACGC 50 0.0025775789 22.004732 13 TAGACCC 80 3.6149086E-6 22.001297 5 >>END_MODULE