FastQCFastQC Report
Mon 6 Feb 2017
SRR2077089_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077089_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849941
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.024.0203271
GTATCAA29400.021.9303511
TCAACGC35000.017.9817074
TATCAAC36550.017.6409022
CAACGCA36300.017.27625
ATCAACG36600.017.1962243
AACGCAG38100.016.5125396
AATAGCG1259.431169E-615.8431865
GTACATG65000.015.098951
TACATGG64900.014.6819512
ATAGCGT1057.169283E-414.6649816
CGCAATA1057.170237E-414.66472336
GTATAGT3550.014.2568891
GCGATAC1554.57203E-614.19341314
ACATGGG66400.014.019113
GTGTAGC10950.013.6653281
TGCGATA1302.3338263E-413.53738213
ACGCAGA45950.013.5000817
TATACTG6700.013.13697055
ATAGGAC4800.012.8370423
Produced by FastQC (version 0.11.5)