##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077089_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2849941 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.75254680710934 31.0 30.0 34.0 16.0 34.0 2 28.820273472328022 31.0 30.0 34.0 16.0 34.0 3 28.877649396952428 31.0 30.0 34.0 16.0 34.0 4 32.25043360546762 35.0 33.0 37.0 19.0 37.0 5 32.21445847475439 35.0 33.0 37.0 19.0 37.0 6 32.249609728762806 36.0 33.0 37.0 19.0 37.0 7 32.1016256126004 36.0 33.0 37.0 18.0 37.0 8 32.02859287262438 36.0 33.0 37.0 17.0 37.0 9 33.32624464857343 38.0 33.0 39.0 17.0 39.0 10 33.26479390275097 38.0 33.0 39.0 17.0 39.0 11 33.31420369755023 38.0 33.0 39.0 17.0 39.0 12 33.20084135075077 38.0 33.0 39.0 17.0 39.0 13 33.1439219268048 38.0 33.0 39.0 17.0 39.0 14 34.0473771211404 39.0 33.0 41.0 16.0 41.0 15 34.08996537121295 39.0 33.0 41.0 17.0 41.0 16 34.05538535710038 39.0 33.0 41.0 16.0 41.0 17 33.89559889134547 39.0 33.0 41.0 13.0 41.0 18 33.8723717438361 39.0 33.0 41.0 14.0 41.0 19 34.002554438846275 39.0 33.0 41.0 16.0 41.0 20 33.988746433698104 39.0 33.0 41.0 15.0 41.0 21 33.934940407538264 39.0 33.0 41.0 13.0 41.0 22 33.856077722310744 39.0 33.0 41.0 11.0 41.0 23 33.81794219599634 39.0 33.0 41.0 11.0 41.0 24 33.82311598731342 39.0 33.0 41.0 11.0 41.0 25 33.7296744037859 39.0 33.0 41.0 10.0 41.0 26 33.67469502000217 39.0 33.0 41.0 10.0 41.0 27 33.61778191197642 39.0 33.0 41.0 10.0 41.0 28 33.51033617888932 39.0 33.0 41.0 9.0 41.0 29 33.49384039880124 39.0 33.0 41.0 9.0 41.0 30 33.335650808209714 38.0 33.0 40.0 8.0 41.0 31 33.3290197937431 38.0 33.0 40.0 8.0 41.0 32 33.19818620806536 38.0 32.0 40.0 8.0 41.0 33 33.154138278652084 38.0 32.0 40.0 7.0 41.0 34 33.0706846211904 38.0 32.0 40.0 2.0 41.0 35 33.00678470185874 38.0 32.0 40.0 2.0 41.0 36 32.91605299899191 38.0 32.0 40.0 2.0 41.0 37 32.82406723507609 38.0 32.0 40.0 2.0 41.0 38 32.73825949379303 38.0 32.0 40.0 2.0 41.0 39 32.628213706880246 38.0 32.0 40.0 2.0 41.0 40 32.5601252797865 38.0 32.0 40.0 2.0 41.0 41 32.45252129780933 38.0 31.0 40.0 2.0 41.0 42 32.30993132840294 38.0 31.0 40.0 2.0 41.0 43 32.21879856460186 38.0 31.0 40.0 2.0 41.0 44 32.072550975616686 38.0 31.0 40.0 2.0 41.0 45 31.931469809375002 38.0 31.0 40.0 2.0 41.0 46 31.79171042488248 38.0 31.0 40.0 2.0 41.0 47 31.633872420516777 38.0 31.0 40.0 2.0 41.0 48 31.472785576964576 38.0 30.0 40.0 2.0 41.0 49 31.303676812958585 38.0 30.0 40.0 2.0 41.0 50 31.096305853349246 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.4765945237250122 1101 2 1.536472912212993 1101 3 1.6088087877327197 1101 4 1.6894060553131638 1101 5 1.7670133570157773 1101 6 1.6802916565916206 1101 7 1.7961055419707321 1101 8 1.7830254963797287 1101 9 2.1988690873735237 1101 10 2.156170581722172 1101 11 2.068163703962874 1101 12 2.131227552556126 1101 13 2.0976224054461525 1101 14 2.4868150415962234 1101 15 2.425816038004143 1101 16 2.39403831534203 1101 17 2.5682631096827606 1101 18 2.548396316400975 1101 19 2.514492074502833 1101 20 2.419218007730848 1101 21 2.5574948842916285 1101 22 2.5573572402895834 1101 23 2.5031032431625775 1101 24 2.5421117822446178 1101 25 2.490965840953912 1101 26 2.5218424536904465 1101 27 2.444483435497766 1101 28 2.5371020112190052 1101 29 2.5480506219690113 1101 30 2.725525153094395 1101 31 2.5920515326217455 1101 32 2.7279545077013623 1101 33 2.754223911562214 1101 34 2.7956139364714616 1101 35 2.886903169429253 1101 36 2.8199622708809926 1101 37 2.9329283634001726 1101 38 2.8374700975491365 1101 39 2.906251233997601 1101 40 2.783171415319366 1101 41 3.025323232189436 1101 42 3.000877626981989 1101 43 3.0430554216053096 1101 44 2.994994188458392 1101 45 3.0311047311649375 1101 46 3.0607026461108475 1101 47 2.9904492071063977 1101 48 3.1441064056755472 1101 49 3.0346160089511436 1101 50 3.169200569512192 1102 1 0.6512113270652193 1102 2 0.5751698853441276 1102 3 0.6348385184544192 1102 4 0.7137038244947718 1102 5 0.6364397933456729 1102 6 0.6040846726497549 1102 7 0.8040588792186796 1102 8 0.6978096618128191 1102 9 0.8289833911552762 1102 10 0.907458632607522 1102 11 0.7832410870902962 1102 12 0.8459262761760655 1102 13 0.8568378283560847 1102 14 1.1096195940572002 1102 15 0.9220965197564013 1102 16 1.071273008234293 1102 17 1.3220806060780532 1102 18 1.2833287108029836 1102 19 1.1859658686954404 1102 20 1.0051418003411996 1102 21 0.9546905860734611 1102 22 1.1368956164840185 1102 23 1.0275357069404194 1102 24 0.9963142671705896 1102 25 1.0234472535053385 1102 26 1.036369860930364 1102 27 0.8763289164534953 1102 28 1.0343588935584123 1102 29 0.9213241036593374 1102 30 0.9869812936398219 1102 31 1.0457789351013744 1102 32 1.031935192820832 1102 33 1.0746868336289452 1102 34 1.1591308502450062 1102 35 1.1732848425254687 1102 36 1.1624972556385984 1102 37 1.0639390083303368 1102 38 1.085382128454242 1102 39 1.2123391592761763 1102 40 1.2566010129151977 1102 41 1.231852647406626 1102 42 1.260028407304567 1102 43 1.1125627017400461 1102 44 1.098620853985473 1102 45 1.102479073142618 1102 46 1.0747747152930245 1102 47 1.161914616695622 1102 48 1.0055849141790212 1102 49 1.0856022411619257 1102 50 1.0267514322743523 1103 1 0.3089588722132177 1103 2 0.4499365810111726 1103 3 0.45967085743001945 1103 4 0.528327073922938 1103 5 0.4845714642738699 1103 6 0.47617582661344215 1103 7 0.3590870102947292 1103 8 0.5608525086043059 1103 9 0.6071228179439601 1103 10 0.5822734969956329 1103 11 0.5554947470455645 1103 12 0.4450343345162864 1103 13 0.4703135857741074 1103 14 0.5470992262186058 1103 15 0.395145524259668 1103 16 0.4670750705543014 1103 17 0.6256866031723547 1103 18 0.46878761141122993 1103 19 0.6692758376613739 1103 20 0.572844761389689 1103 21 0.46335098219007875 1103 22 0.3432782257934335 1103 23 0.4864526625780954 1103 24 0.3527883569192696 1103 25 0.42643136538141135 1103 26 0.5125261079390029 1103 27 0.46090397237394143 1103 28 0.47174073648825043 1103 29 0.4265102968349126 1103 30 0.5676582949227083 1103 31 0.34928920851817225 1103 32 0.5503429386250147 1103 33 0.518630292686062 1103 34 0.5601705675450148 1103 35 0.3856370801638107 1103 36 0.5118836621445979 1103 37 0.5504514123366633 1103 38 0.39142128923496955 1103 39 0.4554546970467257 1103 40 0.45213851906907365 1103 41 0.4276399675721123 1103 42 0.46040733615071616 1103 43 0.3646420950289766 1103 44 0.3273122735440026 1103 45 0.3754538475339473 1103 46 0.3688321870335898 1103 47 0.2822592266558672 1103 48 0.16935349044453218 1103 49 0.2660391306357788 1103 50 0.3128683830019199 1104 1 0.32519697189981045 1104 2 0.34492129581092 1104 3 0.34064458607037906 1104 4 0.244158514214881 1104 5 0.33791606918645556 1104 6 0.4092599518948674 1104 7 0.44538975589374274 1104 8 0.5131796699247673 1104 9 0.5001386367597149 1104 10 0.40512158741042725 1104 11 0.5283450387009978 1104 12 0.4892139755918521 1104 13 0.32588106024240204 1104 14 0.5090570837861179 1104 15 0.5067964889987593 1104 16 0.45526108106411556 1104 17 0.538346896825523 1104 18 0.4721878506996404 1104 19 0.5578124257833821 1104 20 0.4492809175111816 1104 21 0.5098251801472173 1104 22 0.4773386383698437 1104 23 0.5222455512465061 1104 24 0.5508024032377676 1104 25 0.45488484222438785 1104 26 0.43295864737866196 1104 27 0.5078356500061645 1104 28 0.47162093377463066 1104 29 0.44030044307961447 1104 30 0.40958743460883795 1104 31 0.5397530250051688 1104 32 0.482094917044364 1104 33 0.3761654359658664 1104 34 0.35147505854122585 1104 35 0.5383633486549328 1104 36 0.5797720710556504 1104 37 0.6195895941568921 1104 38 0.592461747424359 1104 39 0.6146397788426299 1104 40 0.5493099222891686 1104 41 0.5774852952637275 1104 42 0.49953046414383806 1104 43 0.5526735022588554 1104 44 0.5333604135774692 1104 45 0.5517441797719798 1104 46 0.4604481292926721 1104 47 0.4249653761654564 1104 48 0.453432568825729 1104 49 0.5531260087718728 1104 50 0.655885538924359 1105 1 0.43934023211163264 1105 2 0.4341923815888613 1105 3 0.3048557055254264 1105 4 0.30201563166124856 1105 5 0.4110369846547073 1105 6 0.3356737976038602 1105 7 0.3577011800441099 1105 8 0.4645988300870272 1105 9 0.42368994781812575 1105 10 0.3718195704966547 1105 11 0.3855811333237611 1105 12 0.41390504664151706 1105 13 0.5181455718017389 1105 14 0.6436556334274712 1105 15 0.5701789899508967 1105 16 0.4828910520408485 1105 17 0.5539164273710071 1105 18 0.47133705350257316 1105 19 0.4535889936634163 1105 20 0.46519679628329413 1105 21 0.6684616344176675 1105 22 0.5954500721555362 1105 23 0.5760096914968926 1105 24 0.5923759195504985 1105 25 0.5406260453623233 1105 26 0.6369109817976977 1105 27 0.6619015775316583 1105 28 0.6413561862758641 1105 29 0.5959217872422471 1105 30 0.6409944333245505 1105 31 0.3902552977881797 1105 32 0.5517955347229346 1105 33 0.599678011857371 1105 34 0.6281461257270635 1105 35 0.59667639917825 1105 36 0.5793627151751366 1105 37 0.6667716640785244 1105 38 0.6886014429539244 1105 39 0.7016308599638208 1105 40 0.7569113692163185 1105 41 0.7332824792481105 1105 42 0.7107438490633129 1105 43 0.7477586726126475 1105 44 0.7744691369677668 1105 45 0.9066373105515382 1105 46 0.8527191451805791 1105 47 0.6830562132337725 1105 48 0.8714920843046237 1105 49 0.9026036801679602 1105 50 0.8152066925227714 1106 1 0.2807819581313993 1106 2 0.1085559059390846 1106 3 0.13202489858600686 1106 4 0.07792348033508034 1106 5 0.15710514294200095 1106 6 -0.020712301246582854 1106 7 0.04416689588911993 1106 8 0.175558912704048 1106 9 0.20338743461904585 1106 10 0.21687853229043697 1106 11 0.15064833337910954 1106 12 0.2031874993523246 1106 13 0.3009204973161701 1106 14 0.3474473131613678 1106 15 0.2860721995477391 1106 16 0.32628024411464907 1106 17 0.4110661438806673 1106 18 0.11997718268334978 1106 19 0.315223912398956 1106 20 0.17421820309783698 1106 21 0.3132975695709419 1106 22 0.2616128589185891 1106 23 0.28113446486435834 1106 24 0.2446364036268207 1106 25 0.22433228385111192 1106 26 0.26079502155534584 1106 27 0.3750409809023125 1106 28 0.2809585190763002 1106 29 0.3050452743974219 1106 30 0.2491654730941093 1106 31 0.176402088183778 1106 32 0.29093111461772025 1106 33 0.3617871504864425 1106 34 0.3784782176500343 1106 35 0.23722269157301668 1106 36 0.34694770792837915 1106 37 0.2022449605230463 1106 38 0.29104804406610896 1106 39 0.2847547868206064 1106 40 0.5156316295741163 1106 41 0.3344042098652764 1106 42 0.2939388540832084 1106 43 0.2369850378474112 1106 44 0.4432706060172862 1106 45 0.4164913715815928 1106 46 0.38147262512774915 1106 47 0.3678815547161456 1106 48 0.4665769915183198 1106 49 0.38180840248013226 1106 50 0.4562143313458904 1107 1 0.004532976994482851 1107 2 -0.08868421847919095 1107 3 -0.030342018772113732 1107 4 -0.15635898927975234 1107 5 -0.1814175623289671 1107 6 -0.12566535579039595 1107 7 -0.16002587454705264 1107 8 -0.08995883785921421 1107 9 -0.03593366142965948 1107 10 -0.23970494135109277 1107 11 -0.1611059772277983 1107 12 -0.2611249036122629 1107 13 -0.05777100369155619 1107 14 -0.21750256395863232 1107 15 -0.055331061766793255 1107 16 -0.17054051079529842 1107 17 -0.07820654829113494 1107 18 -0.04814454234956855 1107 19 -0.12156342632366801 1107 20 0.0028838896385323665 1107 21 0.00335724853906072 1107 22 -0.31515162700981847 1107 23 -0.38103071129913957 1107 24 -0.298902568428943 1107 25 -0.261439918397798 1107 26 0.02553606851929402 1107 27 -0.006870651410913808 1107 28 -0.18422109082907667 1107 29 -0.02981106485729157 1107 30 -0.11524987391896246 1107 31 -0.06366110724543717 1107 32 -0.11000429205469686 1107 33 -0.04402226740894832 1107 34 -0.05945372215600031 1107 35 -0.08751702726139854 1107 36 -0.1575778794397067 1107 37 -0.11992718131558888 1107 38 -0.12202377663976449 1107 39 -0.011259227467711241 1107 40 -0.01256226276725414 1107 41 -0.060422221257340425 1107 42 -0.1809097629668912 1107 43 -0.2971364030666521 1107 44 -0.0935261472944191 1107 45 -0.017765796325079464 1107 46 -0.20005757622957532 1107 47 -0.07152926012494731 1107 48 -0.17033467021549598 1107 49 -0.16324741118273423 1107 50 -0.1211793349332666 1108 1 -0.013995731373800169 1108 2 0.021962710349555437 1108 3 0.06866236365472744 1108 4 -0.0339804765269065 1108 5 -0.015811332551614043 1108 6 -0.1299409376060865 1108 7 -0.10756140497029065 1108 8 -0.13070596951543934 1108 9 -0.16912845237845886 1108 10 -0.16653924786454155 1108 11 -0.031754045736825276 1108 12 -0.21110896589578942 1108 13 -0.20431964221880605 1108 14 -0.2739327647738534 1108 15 -0.13571769830316072 1108 16 -0.10804151246741611 1108 17 -0.20996961588307528 1108 18 -0.18787646817774828 1108 19 -0.2771226620968079 1108 20 -0.40299187452600194 1108 21 -0.2645121369773875 1108 22 -0.41467776931671096 1108 23 -0.4992992520275692 1108 24 -0.34272327535125413 1108 25 -0.2481644238388796 1108 26 -0.3432003643882595 1108 27 -0.305937003953197 1108 28 -0.47321373811049483 1108 29 -0.5062704343647937 1108 30 -0.3821077931832946 1108 31 -0.3448398215792352 1108 32 -0.1645077208896808 1108 33 -0.22766571843207828 1108 34 -0.22020028397208335 1108 35 -0.16613715935746853 1108 36 -0.3733229187141589 1108 37 -0.22760203765354703 1108 38 -0.2404045046461647 1108 39 -0.21179665795166613 1108 40 -0.1571609795584834 1108 41 -0.11292764809431333 1108 42 -0.02795220359222128 1108 43 -0.18954168049867093 1108 44 -0.21709954359508643 1108 45 -0.09850858295022036 1108 46 -0.13278082446084483 1108 47 -0.2222786889492454 1108 48 -0.36104057749894025 1108 49 -0.4113949855335157 1108 50 -0.32843731759231787 1109 1 -0.17753405471644257 1109 2 -0.14821213450935034 1109 3 -0.28991573442602103 1109 4 -0.24736689986033156 1109 5 -0.2466704313275514 1109 6 -0.26898629230574045 1109 7 -0.2730359630253574 1109 8 -0.3692998257203861 1109 9 -0.3624144434630665 1109 10 -0.43619498959529324 1109 11 -0.3084493574912486 1109 12 -0.43249472784413 1109 13 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2216 38 -1.2421135117902757 2216 39 -1.0668115311364552 2216 40 -1.239334117550559 2216 41 -1.1321341769908067 2216 42 -1.1717924283242809 2216 43 -1.2430370580969594 2216 44 -1.3525651663159834 2216 45 -1.4375181078641717 2216 46 -1.5832009139621235 2216 47 -1.5385886262101245 2216 48 -1.657786657673899 2216 49 -1.5726632361239652 2216 50 -1.6043783245947587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 168437.0 3 8280.0 4 15176.0 5 11831.0 6 9767.0 7 8015.0 8 7408.0 9 6620.0 10 6296.0 11 6291.0 12 6709.0 13 6855.0 14 7218.0 15 7774.0 16 8456.0 17 9032.0 18 9891.0 19 11205.0 20 13773.0 21 17448.0 22 21384.0 23 24112.0 24 26058.0 25 28046.0 26 31370.0 27 35565.0 28 40297.0 29 45986.0 30 54093.0 31 64614.0 32 78944.0 33 97377.0 34 123558.0 35 158409.0 36 208610.0 37 283488.0 38 441765.0 39 739783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.39140316701767 17.015441765989344 12.074961624730426 25.51819344226256 2 16.329341721150957 19.14906170890077 38.59935574143119 25.92224082851708 3 19.8728033249423 23.16483479430028 28.06710859204513 28.895253288712286 4 13.119567832612411 15.162387668617813 36.101466608360596 35.61657789040918 5 14.579636064018061 36.790084779782944 34.02560146442596 14.604677691773036 6 34.29189139088286 35.356699736615376 16.52610358578046 13.825305286721301 7 29.888127924415958 31.105394125610157 20.30267880714723 18.70379914282665 8 27.127968834262088 34.81045945308287 19.14687443825274 18.9146972744023 9 26.57532322762422 15.76831184334103 18.391103230959633 39.265261698075115 10 14.575621338832084 28.842885762960453 33.27614829074996 23.305344607457506 11 36.63090011988493 22.397452752526274 22.198756215849034 18.772890911739772 12 23.720207155654283 24.326535226537437 29.460557604150978 22.492700013657302 13 28.6765826193253 21.280657643355706 25.03923153239243 25.003528204926567 14 22.215001941401276 20.628985928526944 26.332036491322675 30.823975638749108 15 24.240191234030334 28.68710564037175 22.68404502523656 24.388658100361354 16 24.366004040216797 27.531437366458338 24.454265293526596 23.64829329979827 17 23.218551895292762 27.47115133799724 25.892088854124722 23.41820791258528 18 23.31036342265244 27.164891846688132 26.26149607283339 23.263248657826036 19 24.094120274157582 26.645010450669087 26.472652531842677 22.788216743330654 20 24.213141290111903 26.292622464422028 26.336372925276603 23.157863320189467 21 24.340866736406745 26.03776990480445 26.347526808316978 23.273836550471835 22 24.028434953796538 26.018059981275606 26.14760666970546 23.8058983952224 23 23.837880462488467 26.015608213341274 26.07740067213343 24.069110652036834 24 23.472217393884552 26.107506537569208 26.328065002417677 24.09221106612856 25 23.903946508965507 25.954143518355938 26.307636659900684 23.834273312777867 26 23.65397311457909 26.29981317863213 26.353331179503027 23.692882527285754 27 23.888460680844936 26.37671032047714 26.08318394822643 23.651645050451492 28 23.768652234475 26.360138885084044 26.092301121856 23.778907758584964 29 23.721762607486987 26.45129820189538 26.24501589785479 23.58192329276284 30 23.943936969826368 26.322387707486726 26.15625083402045 23.577424488666452 31 23.956508077497634 26.04133277394417 26.077423907567375 23.92473524099082 32 23.66462113127001 26.40783856653373 26.063303937538617 23.864236364657643 33 23.74279111063677 26.166649054396636 26.12490621905053 23.965653615916064 34 23.958280309000944 26.24422729578538 26.157726691088577 23.639765704125104 35 23.94315018500204 26.28563163125841 26.116200027594953 23.6550181561446 36 23.952299937297735 26.31069147326447 26.00462297542956 23.732385614008233 37 23.9667576100897 26.006963473886625 26.19103876675522 23.835240149268454 38 23.873698030941842 26.148734277198738 26.220138525646203 23.757429166213225 39 23.958165112363417 26.427379797193968 26.03270707152978 23.581748018912833 40 24.115790283005385 26.107265470225123 26.252961759814575 23.523982486954917 41 23.82399297937692 26.247863097849933 26.198929354980255 23.72921456779289 42 24.026277638733365 26.113359613312227 26.110867344610554 23.74949540334385 43 23.708656508528456 26.01280672391618 26.270913951620606 24.00762281593476 44 23.822678413410355 26.247752204971526 26.107382385430462 23.822186996187654 45 23.938802586043508 26.187095053244136 26.013674301719114 23.86042805899324 46 23.872593768744185 26.08906236344933 25.92764959636279 24.1106942714437 47 23.7617538354621 26.235332933202766 26.05548477222392 23.94742845911122 48 23.78073241516746 26.324401215626253 26.132946047547566 23.76192032165872 49 23.844396837340938 26.157428205617762 26.014267624299464 23.983907332741833 50 24.04894556588748 26.17736309694701 26.06020765083325 23.713483686332264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1651.0 1 1253.5 2 856.0 3 806.5 4 757.0 5 737.0 6 717.0 7 679.0 8 641.0 9 738.0 10 835.0 11 1050.5 12 1266.0 13 1955.5 14 2645.0 15 3515.0 16 4385.0 17 5022.0 18 5659.0 19 6319.0 20 6979.0 21 8458.0 22 9937.0 23 13295.0 24 16653.0 25 22133.5 26 27614.0 27 33952.0 28 40290.0 29 47458.5 30 54627.0 31 63026.5 32 71426.0 33 79687.5 34 87949.0 35 97797.5 36 107646.0 37 116299.0 38 124952.0 39 134664.0 40 144376.0 41 153298.0 42 162220.0 43 169610.0 44 177000.0 45 185960.0 46 194920.0 47 201326.5 48 207733.0 49 209105.5 50 210478.0 51 210009.5 52 209541.0 53 203957.5 54 198374.0 55 190472.5 56 182571.0 57 169816.0 58 157061.0 59 143910.0 60 130759.0 61 115092.0 62 99425.0 63 85703.5 64 71982.0 65 60563.5 66 49145.0 67 40851.5 68 32558.0 69 26861.5 70 21165.0 71 17100.5 72 13036.0 73 10782.0 74 8528.0 75 6653.5 76 4779.0 77 3803.0 78 2827.0 79 2245.0 80 1663.0 81 1309.0 82 955.0 83 763.5 84 572.0 85 446.5 86 321.0 87 255.5 88 190.0 89 147.5 90 105.0 91 84.5 92 64.0 93 50.5 94 37.0 95 28.0 96 19.0 97 15.0 98 11.0 99 26.0 100 41.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08435262344027472 2 0.09252823128619153 3 0.10014242400105827 4 0.10161613872006472 5 0.09410721134226989 6 0.05736960870418019 7 0.0529484645471608 8 0.0591240309887117 9 0.06021177280512123 10 0.04540444872367533 11 0.048281701270307 12 0.05782575849815838 13 0.05147474982815434 14 0.05203616495920442 15 0.05242213786180135 16 0.05540465574550491 17 0.054106383254951596 18 0.05533447885412364 19 0.0630890253517529 20 0.0691593264562319 21 0.0780367032159613 22 0.08137010555657118 23 0.059545092336999256 24 0.0761419271486673 25 0.08284382027557764 26 0.08073851353413983 27 0.07565068890899847 28 0.09456336113624808 29 0.08375611986353401 30 0.08031745218585226 31 0.05600115932224562 32 0.05126421915401056 33 0.05431691392909537 34 0.049334354641025896 35 0.05593098243086436 36 0.05470288683169231 37 0.057895935389539646 38 0.048106259041853845 39 0.052808110764398286 40 0.05438709082047664 41 0.041790338817540434 42 0.039685032076102626 43 0.03947450140195885 44 0.03642180662687403 45 0.0281058449981947 46 0.02540403468001618 47 0.030562036196538803 48 0.02491279644034736 49 0.02431629286360665 50 0.025790007582613118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2849941.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.12827462417789 #Duplication Level Percentage of deduplicated Percentage of total 1 79.93478318563632 52.85949294523875 2 11.637849118916343 15.391817651408932 3 3.898098011958035 7.733234875401685 4 1.6707675272759133 4.419398955074408 5 0.8490499800493421 2.8073105125177826 6 0.5126418594769583 2.0340073000404906 7 0.3315069939819367 1.5345389876511235 8 0.22255996648112825 1.1774005267049499 9 0.16389989800463892 0.9754575719512958 >10 0.7350996008613512 8.453306408321014 >50 0.03396630732961428 1.5127851760672757 >100 0.009510953100569326 0.9554523401299148 >500 2.1327754229977E-4 0.10122913368775938 >1k 5.331938557179384E-5 0.0445676158046139 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.5088445690630086E-5 0.0 5 0.0 0.0 0.0 3.5088445690630086E-5 7.017689138126017E-5 6 0.0 0.0 0.0 7.017689138126017E-5 1.0526533707189026E-4 7 0.0 0.0 0.0 7.017689138126017E-5 1.0526533707189026E-4 8 0.0 0.0 0.0 7.017689138126017E-5 1.0526533707189026E-4 9 0.0 0.0 0.0 1.0526533707189026E-4 1.0526533707189026E-4 10 0.0 3.5088445690630086E-5 0.0 1.0526533707189026E-4 1.0526533707189026E-4 11 0.0 3.5088445690630086E-5 0.0 1.0526533707189026E-4 1.0526533707189026E-4 12 0.0 3.5088445690630086E-5 0.0 1.0526533707189026E-4 2.1053067414378052E-4 13 0.0 3.5088445690630086E-5 0.0 1.0526533707189026E-4 2.807075655250407E-4 14 0.0 3.5088445690630086E-5 0.0 1.0526533707189026E-4 2.807075655250407E-4 15 0.0 3.5088445690630086E-5 0.0 1.0526533707189026E-4 2.807075655250407E-4 16 0.0 3.5088445690630086E-5 0.0 1.0526533707189026E-4 2.807075655250407E-4 17 0.0 3.5088445690630086E-5 0.0 1.0526533707189026E-4 2.807075655250407E-4 18 0.0 3.5088445690630086E-5 0.0 1.7544222845315043E-4 2.807075655250407E-4 19 0.0 3.5088445690630086E-5 0.0 1.7544222845315043E-4 2.807075655250407E-4 20 0.0 3.5088445690630086E-5 0.0 1.7544222845315043E-4 3.157960112156708E-4 21 0.0 7.017689138126017E-5 0.0 2.456191198344106E-4 3.157960112156708E-4 22 0.0 7.017689138126017E-5 0.0 2.807075655250407E-4 3.157960112156708E-4 23 0.0 1.4035378276252035E-4 0.0 2.807075655250407E-4 3.157960112156708E-4 24 3.5088445690630086E-5 1.4035378276252035E-4 0.0 3.5088445690630086E-4 3.157960112156708E-4 25 3.5088445690630086E-5 1.4035378276252035E-4 0.0 3.5088445690630086E-4 3.157960112156708E-4 26 3.5088445690630086E-5 1.4035378276252035E-4 0.0 5.263266853594513E-4 3.5088445690630086E-4 27 3.5088445690630086E-5 1.4035378276252035E-4 0.0 8.07034250884492E-4 3.5088445690630086E-4 28 7.017689138126017E-5 2.1053067414378052E-4 0.0 0.001614068501768984 3.5088445690630086E-4 29 1.4035378276252035E-4 2.456191198344106E-4 0.0 0.003649198351825529 3.5088445690630086E-4 30 1.4035378276252035E-4 2.456191198344106E-4 0.0 0.0065615393441478265 3.5088445690630086E-4 31 1.4035378276252035E-4 2.456191198344106E-4 0.0 0.013473963145201953 3.5088445690630086E-4 32 1.4035378276252035E-4 2.456191198344106E-4 0.0 0.021614482545428134 3.5088445690630086E-4 33 1.4035378276252035E-4 2.807075655250407E-4 0.0 0.03200066246985464 3.5088445690630086E-4 34 1.4035378276252035E-4 2.807075655250407E-4 0.0 0.04659745587715675 4.561497939781911E-4 35 1.4035378276252035E-4 3.157960112156708E-4 0.0 0.06315920224313415 4.561497939781911E-4 36 2.456191198344106E-4 3.157960112156708E-4 0.0 0.08554563059375615 4.561497939781911E-4 37 2.456191198344106E-4 3.5088445690630086E-4 0.0 0.11400236004885715 4.561497939781911E-4 38 2.807075655250407E-4 3.5088445690630086E-4 0.0 0.1456521380618055 4.561497939781911E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1365 0.0 24.020327 1 GTATCAA 2940 0.0 21.930351 1 TCAACGC 3500 0.0 17.981707 4 TATCAAC 3655 0.0 17.640902 2 CAACGCA 3630 0.0 17.2762 5 ATCAACG 3660 0.0 17.196224 3 AACGCAG 3810 0.0 16.512539 6 AATAGCG 125 9.431169E-6 15.843186 5 GTACATG 6500 0.0 15.09895 1 TACATGG 6490 0.0 14.681951 2 ATAGCGT 105 7.169283E-4 14.664981 6 CGCAATA 105 7.170237E-4 14.664723 36 GTATAGT 355 0.0 14.256889 1 GCGATAC 155 4.57203E-6 14.193413 14 ACATGGG 6640 0.0 14.01911 3 GTGTAGC 1095 0.0 13.665328 1 TGCGATA 130 2.3338263E-4 13.537382 13 ACGCAGA 4595 0.0 13.500081 7 TATACTG 670 0.0 13.1369705 5 ATAGGAC 480 0.0 12.837042 3 >>END_MODULE