FastQCFastQC Report
Mon 6 Feb 2017
SRR2077089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849941
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.024.0021841
GTATCAA36300.023.1536221
TATCAAC49750.016.6728732
TCAACGC50250.016.5075534
GGACCGT2151.2732926E-1116.3741576
ATCAACG51000.016.3076523
CAACGCA50900.016.2108635
TACGGTA850.002979461615.5308252
AACGCAG53050.015.42896
GTACATG62850.014.7380161
TAGCGTG1057.154301E-414.669037
TATACCG900.00435626914.669035
TCGATCG1201.184884E-414.66233841
TACATGG62800.014.6446442
GTATTAC3450.014.0302621
ATACGCG1100.001020463813.99660539
GCACCGT3850.013.7160146
ACATGGG63750.013.6673653
TCTATAC4350.013.6566553
CCGTACC1302.3287215E-413.5408819
Produced by FastQC (version 0.11.5)