FastQCFastQC Report
Mon 6 Feb 2017
SRR2077088_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077088_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438965
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.025.452291
GTATCAA38700.021.8361871
CGTCGCG654.9402716E-420.30395332
CGTAGAC1102.7900187E-618.005923
TAGCGCG1408.374445E-817.28714229
ATCAACG50500.016.5163213
TCAACGC50950.016.2837244
TATCAAC51650.016.2764052
CAACGCA51750.016.1147295
AACGCAG53200.015.7133266
CGAACGT2001.2732926E-915.4047484
TATACTG5050.015.2500355
GGACCGT1901.0786607E-815.0517136
GTACATG79500.014.5604631
GTATAGA4100.014.4922711
CTTACAC4750.014.3626163
CGACAGG4600.014.34489134
GTGTACG2803.6379788E-1214.1472191
TACATGG79050.014.1146972
GTCGGTT1251.6735715E-414.07856212
Produced by FastQC (version 0.11.5)