##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077088_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2438965
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.05035660618336	31.0	28.0	33.0	15.0	34.0
2	28.21385095727081	31.0	28.0	34.0	16.0	34.0
3	28.29037932073646	31.0	28.0	34.0	16.0	34.0
4	31.61145854901567	35.0	32.0	37.0	16.0	37.0
5	31.53531723497467	35.0	32.0	37.0	16.0	37.0
6	31.61598505923619	35.0	32.0	37.0	16.0	37.0
7	31.424166808461788	35.0	32.0	37.0	11.0	37.0
8	31.360551709434123	35.0	32.0	37.0	10.0	37.0
9	32.575911913455094	37.0	32.0	39.0	10.0	39.0
10	32.54910341066805	37.0	32.0	39.0	10.0	39.0
11	32.531298727124	37.0	32.0	39.0	10.0	39.0
12	32.44049340601444	37.0	32.0	39.0	10.0	39.0
13	32.37066255563323	37.0	32.0	39.0	8.0	39.0
14	33.233845504138024	38.0	32.0	41.0	8.0	41.0
15	33.27504494734447	38.0	32.0	41.0	8.0	41.0
16	33.239914881927376	38.0	32.0	41.0	8.0	41.0
17	33.07812002222254	38.0	32.0	41.0	8.0	41.0
18	33.05698318754062	38.0	32.0	41.0	8.0	41.0
19	33.206014436451525	39.0	32.0	41.0	8.0	41.0
20	33.18511171747032	39.0	32.0	41.0	8.0	41.0
21	33.14246329898133	39.0	32.0	41.0	7.0	41.0
22	33.067070253160665	38.0	32.0	41.0	2.0	41.0
23	33.02458747870511	38.0	32.0	40.0	2.0	41.0
24	33.03246951063258	38.0	32.0	41.0	2.0	41.0
25	32.940406278892894	38.0	32.0	40.0	2.0	41.0
26	32.88581631962738	38.0	32.0	40.0	2.0	41.0
27	32.828103724325686	38.0	32.0	40.0	2.0	41.0
28	32.71795618223304	38.0	32.0	40.0	2.0	41.0
29	32.69526786977263	38.0	32.0	40.0	2.0	41.0
30	32.52225800698247	38.0	31.0	40.0	2.0	41.0
31	32.51238496657393	38.0	31.0	40.0	2.0	41.0
32	32.37962537387786	38.0	31.0	40.0	2.0	41.0
33	32.32925728741495	38.0	31.0	40.0	2.0	41.0
34	32.24034990252013	38.0	31.0	40.0	2.0	41.0
35	32.171439524552426	38.0	31.0	40.0	2.0	41.0
36	32.07151845147429	38.0	30.0	40.0	2.0	41.0
37	31.978494976352675	38.0	30.0	40.0	2.0	41.0
38	31.874249117966023	38.0	30.0	40.0	2.0	41.0
39	31.75873618522611	38.0	30.0	40.0	2.0	41.0
40	31.68750392072047	38.0	30.0	40.0	2.0	41.0
41	31.572052899488103	38.0	30.0	40.0	2.0	41.0
42	31.41826307470587	38.0	30.0	40.0	2.0	41.0
43	31.319958260983654	38.0	30.0	40.0	2.0	41.0
44	31.163036779945593	38.0	30.0	40.0	2.0	41.0
45	31.014932563607925	37.0	30.0	40.0	2.0	41.0
46	30.860346089427278	37.0	30.0	40.0	2.0	41.0
47	30.68971551457278	37.0	29.0	40.0	2.0	41.0
48	30.512202922141153	37.0	29.0	40.0	2.0	41.0
49	30.320848802668344	37.0	29.0	40.0	2.0	41.0
50	30.107325443374545	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.9390479330290766
1101	2	2.356775699993218
1101	3	2.4220091194200393
1101	4	2.6448777673337283
1101	5	2.6373391975803173
1101	6	2.7175958300517813
1101	7	2.7392319242788474
1101	8	2.8475616420888272
1101	9	3.062590842075217
1101	10	3.175525704624789
1101	11	3.1780210243984683
1101	12	3.180296456476185
1101	13	3.151992922832221
1101	14	3.5315957823156765
1101	15	3.6519046314505985
1101	16	3.653497102794084
1101	17	3.736841504011281
1101	18	3.644595599187163
1101	19	3.5309560351488116
1101	20	3.73546606731842
1101	21	3.5806942722095556
1101	22	3.763785582218837
1101	23	3.756191398760137
1101	24	3.7705130641392017
1101	25	3.7114181360651557
1101	26	3.8306577754516624
1101	27	3.671436124285677
1101	28	3.84745089841136
1101	29	3.880946398529005
1101	30	3.932336469463884
1101	31	3.9364085581334436
1101	32	4.011792415107806
1101	33	3.9951209353355495
1101	34	4.109563557423435
1101	35	4.052279453284768
1101	36	4.027930738309564
1101	37	4.225746688515386
1101	38	4.212061265926181
1101	39	4.325300810685597
1101	40	4.254125075295121
1101	41	4.298587365335326
1101	42	4.381535947503103
1101	43	4.268713408997581
1101	44	4.195549064481387
1101	45	4.271091652437395
1101	46	4.374596747931797
1101	47	4.24655332570876
1101	48	4.206021578117177
1101	49	4.316758652237844
1101	50	4.50518690794928
1102	1	0.5353594084389144
1102	2	0.7271553783105702
1102	3	0.7254231311738266
1102	4	0.802640487717273
1102	5	0.7609424685360864
1102	6	0.8432917780876572
1102	7	0.9704219954202031
1102	8	0.8940645032085328
1102	9	0.8738777659849006
1102	10	1.098840930421268
1102	11	0.9293839226353953
1102	12	0.9605122003673117
1102	13	1.008753284725202
1102	14	1.3525051544158941
1102	15	1.1996495664954452
1102	16	1.1774686462521728
1102	17	1.4657214143113109
1102	18	1.269469941902905
1102	19	1.2284409562746958
1102	20	1.1357715111786106
1102	21	1.285626803913857
1102	22	1.27604683337875
1102	23	1.2537749125244488
1102	24	1.2177273388066894
1102	25	1.3792721029689545
1102	26	1.3193099560888442
1102	27	1.0960127558971848
1102	28	1.2497958566544796
1102	29	1.13649465092616
1102	30	1.186463827954448
1102	31	1.224163739079934
1102	32	1.2760061871461659
1102	33	1.2587595836498053
1102	34	1.396975597324456
1102	35	1.3238538423970496
1102	36	1.2880799804994822
1102	37	1.309444105774901
1102	38	1.1866929934238613
1102	39	1.367009363113688
1102	40	1.4590769388893037
1102	41	1.284914886213766
1102	42	1.3032127956317794
1102	43	1.1967842797058985
1102	44	1.1057186051556833
1102	45	1.1137494006588646
1102	46	1.1166851719961315
1102	47	1.0134638963861455
1102	48	1.1277467559705663
1102	49	1.1277217669919395
1102	50	1.1146005717290528
1103	1	0.27518986228110265
1103	2	0.3998071658493707
1103	3	0.27161682662081077
1103	4	0.2664224836400919
1103	5	0.44350882617034415
1103	6	0.3213851227410949
1103	7	0.23838435201327002
1103	8	0.36383592788765995
1103	9	0.4459447658486724
1103	10	0.5293379832203158
1103	11	0.304441394878431
1103	12	0.3183109708870262
1103	13	0.20244751536344552
1103	14	0.41596637007850745
1103	15	0.33049455442931475
1103	16	0.31953090845696863
1103	17	0.404065556886259
1103	18	0.26889096226936715
1103	19	0.28911735288060925
1103	20	0.34601744372505294
1103	21	0.1999182046066892
1103	22	0.18024106036796184
1103	23	0.21110678948883788
1103	24	0.20329329139847374
1103	25	0.3484558815652008
1103	26	0.28997113623422166
1103	27	0.33491453127254545
1103	28	0.30475391712136
1103	29	0.40550063047212603
1103	30	0.42471333898425456
1103	31	0.3480222004537197
1103	32	0.5228219512046621
1103	33	0.3956368764578073
1103	34	0.35605490415446894
1103	35	0.3476388366072527
1103	36	0.3209735650159118
1103	37	0.37784337304941573
1103	38	0.3543083575485255
1103	39	0.41469247150450883
1103	40	0.38017925651072204
1103	41	0.33910649806387383
1103	42	0.2949419620957201
1103	43	0.25515859368801586
1103	44	0.2727814451237691
1103	45	0.2646629964629632
1103	46	0.2074601471827222
1103	47	0.13720117951205424
1103	48	0.13218397803601434
1103	49	-0.001565236618169763
1103	50	0.13672388830928028
1104	1	0.27332098528522053
1104	2	0.26159004670962105
1104	3	0.27502440033362063
1104	4	0.26927855592049355
1104	5	0.2707465631769921
1104	6	0.2974412621384239
1104	7	0.15668390556217915
1104	8	0.36591325852808154
1104	9	0.41199551633326337
1104	10	0.2874678040325307
1104	11	0.2805577542175186
1104	12	0.30682311627135306
1104	13	0.3082674639139924
1104	14	0.47761460532539246
1104	15	0.2850665862295614
1104	16	0.39338822219463054
1104	17	0.37482066863285723
1104	18	0.37511713807659675
1104	19	0.3878426717117449
1104	20	0.3557572472536279
1104	21	0.24526472147496037
1104	22	0.2500487116975805
1104	23	0.210870155999757
1104	24	0.19683869157285727
1104	25	0.41482893741327587
1104	26	0.4566885008860382
1104	27	0.47810352718479976
1104	28	0.2931384168171718
1104	29	0.388210558733185
1104	30	0.3535565293441252
1104	31	0.3648140422471826
1104	32	0.5012908772000202
1104	33	0.36118798157095
1104	34	0.4227936546061173
1104	35	0.4852598040586287
1104	36	0.41448754273350374
1104	37	0.43025672581783425
1104	38	0.39556004705244874
1104	39	0.5718967581349617
1104	40	0.4144662781353432
1104	41	0.49556022289888446
1104	42	0.41777494491810785
1104	43	0.509297813958149
1104	44	0.5712904096598841
1104	45	0.4578983668773695
1104	46	0.20434299314628745
1104	47	0.15555294941217213
1104	48	0.17143296845126343
1104	49	0.27006208635321727
1104	50	0.17848989214482813
1105	1	0.3834165706343349
1105	2	0.3542644075621233
1105	3	0.31000575438653044
1105	4	0.40510879060249394
1105	5	0.2827243305072109
1105	6	0.2506266953576386
1105	7	0.3729869627507796
1105	8	0.4154656428070247
1105	9	0.3593799131807174
1105	10	0.3204796169805988
1105	11	0.4485103059194273
1105	12	0.3807543824615891
1105	13	0.3447322527709389
1105	14	0.37844376168164473
1105	15	0.34459188709227107
1105	16	0.3750388146842738
1105	17	0.4230842816971503
1105	18	0.3052785970930927
1105	19	0.22143877018671532
1105	20	0.34375853977073945
1105	21	0.37132155440030346
1105	22	0.3891923417399852
1105	23	0.28073995369209115
1105	24	0.32534036408311806
1105	25	0.3368299391017544
1105	26	0.47947224670967614
1105	27	0.4613023399948162
1105	28	0.4266652347048989
1105	29	0.3270624881502613
1105	30	0.27705558095785676
1105	31	0.15713599445401627
1105	32	0.3991356626475806
1105	33	0.44069989056123404
1105	34	0.4349762178576597
1105	35	0.42847754038022856
1105	36	0.38991418145703705
1105	37	0.4344583275368663
1105	38	0.4135243645410256
1105	39	0.3982473181520163
1105	40	0.41306162988814776
1105	41	0.298386885596134
1105	42	0.2976269809395937
1105	43	0.47576888363273184
1105	44	0.4974230078649029
1105	45	0.5495607841054309
1105	46	0.7265322474213924
1105	47	0.4740448727768616
1105	48	0.6110312515798135
1105	49	0.5622682843436486
1105	50	0.5062190633863963
1106	1	0.047338318157709836
1106	2	-0.06881918908617735
1106	3	-0.07820722226233201
1106	4	-0.015197386127329793
1106	5	0.016652383504762724
1106	6	-0.07799287205987682
1106	7	-0.10727264113721802
1106	8	0.060734272641933984
1106	9	-0.04183572104398081
1106	10	-0.00711065129164723
1106	11	-0.20472279469944965
1106	12	-0.04932521853412908
1106	13	0.08313940232626749
1106	14	0.04736016600472226
1106	15	0.03644641167979756
1106	16	-0.09072384474204398
1106	17	-0.026680103872458005
1106	18	-0.32161642981475325
1106	19	-0.12162298011237738
1106	20	-0.3032396331838143
1106	21	-0.09541442836546565
1106	22	-0.04660255902195587
1106	23	-0.02066984736794808
1106	24	-0.22931861394437902
1106	25	-0.09984952509928746
1106	26	-0.16519230582810707
1106	27	-0.006141475656093576
1106	28	-0.016701941932524278
1106	29	-0.19777164558043125
1106	30	-0.0639788980743532
1106	31	-0.16223718337261772
1106	32	-0.03050086738186053
1106	33	-0.10043637701331676
1106	34	-0.08022066702196184
1106	35	-0.06939100778586038
1106	36	-0.056933330369822954
1106	37	-0.10428482256050131
1106	38	0.040007708217537186
1106	39	-0.0769337259388223
1106	40	0.11510077628260618
1106	41	-0.020793108917995795
1106	42	-0.1084860506044123
1106	43	-0.1275254807449926
1106	44	-0.19694150342563432
1106	45	-0.08018961601230146
1106	46	-0.12960270696803988
1106	47	-0.2587202555346195
1106	48	-0.1945577929957487
1106	49	-0.21055524336819786
1106	50	-0.15988331211266882
1107	1	-0.21953662637733373
1107	2	-0.2553099058031485
1107	3	-0.2865038970368232
1107	4	-0.43477272256702193
1107	5	-0.44906526384594514
1107	6	-0.4878126980263531
1107	7	-0.2752510639571959
1107	8	-0.24706629493752175
1107	9	-0.21649701504498609
1107	10	-0.5875442608126278
1107	11	-0.49622668464171227
1107	12	-0.5937615943847092
1107	13	-0.4105516676487966
1107	14	-0.5599147846374279
1107	15	-0.545151604082271
1107	16	-0.6058946434087886
1107	17	-0.5456946836413223
1107	18	-0.4735047670493202
1107	19	-0.49843602814193133
1107	20	-0.38671284158223784
1107	21	-0.4385113434165291
1107	22	-0.7950163805550474
1107	23	-0.6847172957891203
1107	24	-0.558071217815332
1107	25	-0.6699454210265188
1107	26	-0.3543629938034982
1107	27	-0.5656088546279818
1107	28	-0.6226586158100318
1107	29	-0.5169235637375351
1107	30	-0.5671195266676463
1107	31	-0.5759142690219576
1107	32	-0.4947098679248185
1107	33	-0.48357076013326505
1107	34	-0.5347906931393958
1107	35	-0.44576451094772196
1107	36	-0.6099529380071438
1107	37	-0.58948522162191
1107	38	-0.5370380063571716
1107	39	-0.5314478099821862
1107	40	-0.46526603673202516
1107	41	-0.45073287755398184
1107	42	-0.47238960026618315
1107	43	-0.6883687484682532
1107	44	-0.49995443141232343
1107	45	-0.3267933715027347
1107	46	-0.4336522928774116
1107	47	-0.342015260396451
1107	48	-0.524939277385883
1107	49	-0.4589561827179267
1107	50	-0.45357383340214597
1108	1	-0.25638073994347366
1108	2	-0.29716716521049236
1108	3	-0.2397350615259235
1108	4	-0.32920241937047123
1108	5	-0.3865782908852573
1108	6	-0.48953550192041817
1108	7	-0.6578604428608976
1108	8	-0.3853698721213483
1108	9	-0.6385564446923908
1108	10	-0.5583158779345965
1108	11	-0.5975197079828689
1108	12	-0.6406772855035499
1108	13	-0.6394598617800327
1108	14	-0.8016092384004381
1108	15	-0.789718652342188
1108	16	-0.6617544115357177
1108	17	-0.7800684706269365
1108	18	-0.7702164953133988
1108	19	-0.631609363162795
1108	20	-0.9501772835873865
1108	21	-0.7362465323491847
1108	22	-0.7855096413117977
1108	23	-0.9670792844528293
1108	24	-0.7701433016287709
1108	25	-0.6575123323701888
1108	26	-0.8557647973338689
1108	27	-0.828953424346345
1108	28	-0.8342957200174084
1108	29	-1.0295546163411338
1108	30	-1.0143690158195007
1108	31	-0.9814497667924833
1108	32	-0.7392780336282883
1108	33	-0.7850146265914688
1108	34	-0.8419449902156444
1108	35	-0.888578690200724
1108	36	-1.1143980696467004
1108	37	-1.008577779824254
1108	38	-0.9849363185233813
1108	39	-0.8396393887393749
1108	40	-1.0009550077288338
1108	41	-0.8408094847789265
1108	42	-0.7375537797779614
1108	43	-0.8107724565929395
1108	44	-0.7914556380161066
1108	45	-0.9184413278513901
1108	46	-0.8490505238819281
1108	47	-0.9736906917414245
1108	48	-0.9551142290535068
1108	49	-1.02222654242183
1108	50	-0.951166448300043
1109	1	-0.4373554826022463
1109	2	-0.5450866724912018
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2210	28	-0.31770618232070547
2210	29	-0.304055838200064
2210	30	-0.1987233590799491
2210	31	-0.2736608794380615
2210	32	-0.4331585648962317
2210	33	-0.3256265082612728
2210	34	-0.21150108602611795
2210	35	-0.2985339342315143
2210	36	-0.45082355205349955
2210	37	-0.3805072208119711
2210	38	-0.3456199932533188
2210	39	-0.27783875032168126
2210	40	-0.45385732580384897
2210	41	-0.3218458970033069
2210	42	-0.3626050099752902
2210	43	-0.3676152278645084
2210	44	-0.43159599333706566
2210	45	-0.191789036871036
2210	46	-0.21043557577238303
2210	47	-0.2044244514971929
2210	48	-0.2739154074846226
2210	49	-0.15537712216526955
2210	50	-0.08044641297977151
2211	1	-0.10044268503722975
2211	2	-0.2703936975836818
2211	3	-0.3231303126357652
2211	4	-0.32867743084016254
2211	5	-0.5463218316788669
2211	6	-0.346958837370444
2211	7	-0.26969507940848203
2211	8	-0.18107650099862838
2211	9	-0.2551184057738425
2211	10	-0.23349926863809145
2211	11	-0.1322991026626852
2211	12	-0.321847002109223
2211	13	-0.32335755284897516
2211	14	-0.5227089928531257
2211	15	-0.43727062833838204
2211	16	-0.342583116530065
2211	17	-0.3384239715901103
2211	18	-0.4570967204769829
2211	19	-0.31827357680387536
2211	20	-0.3841740391511621
2211	21	-0.3183721236274337
2211	22	-0.36821694783048997
2211	23	-0.42919756639381745
2211	24	-0.32121818396525725
2211	25	-0.3431864339860837
2211	26	-0.31450280685874077
2211	27	-0.39967961150374975
2211	28	-0.23276692925796283
2211	29	-0.37172332228763594
2211	30	-0.730060073866202
2211	31	-0.6652363729885558
2211	32	-0.33023812905232575
2211	33	-0.48631273267728403
2211	34	-0.4890644694573396
2211	35	-0.329605353649324
2211	36	-0.5695310316075926
2211	37	-0.410399595424785
2211	38	-0.3454379958102187
2211	39	-0.3430088250511467
2211	40	-0.3611188709433293
2211	41	-0.31047911031394904
2211	42	-0.3280032163834363
2211	43	-0.3003321957400793
2211	44	-0.26407440112749825
2211	45	-0.2184429621490942
2211	46	-0.39619036078849135
2211	47	-0.49695095222953256
2211	48	-0.4548670119190561
2211	49	-0.3408152339817718
2211	50	-0.3221901013249031
2212	1	-0.17373907640957142
2212	2	-0.34636277491984657
2212	3	-0.25814090541851087
2212	4	-0.22626853172240402
2212	5	-0.12151400659255884
2212	6	-0.12984815901926083
2212	7	-0.14320722127505547
2212	8	-0.11460788101102182
2212	9	-0.3328019795839978
2212	10	-0.31507166248896823
2212	11	-0.3069958658151464
2212	12	-0.34076352862068404
2212	13	-0.20307004289058028
2212	14	-1.0338606260300764
2212	15	-0.7518189154942618
2212	16	-0.5601931604945349
2212	17	-0.451329757787029
2212	18	-0.3331372622137323
2212	19	-0.3846249385223288
2212	20	-0.24237757401292725
2212	21	-0.4219671783617258
2212	22	-0.438062157473162
2212	23	-0.4989300617465702
2212	24	-0.44081654341172083
2212	25	-0.23298797421800543
2212	26	-0.3966989061181323
2212	27	-0.5499550828678252
2212	28	-0.4370531999492968
2212	29	-0.4700550917839621
2212	30	-0.46723171235429817
2212	31	-0.29717216715219763
2212	32	-0.3324661541917422
2212	33	-0.2661413597464204
2212	34	-0.21009565767840144
2212	35	-0.2577645109477231
2212	36	-0.44469246089560244
2212	37	-0.3388746477985727
2212	38	-0.3483971295035282
2212	39	-0.32612156884743726
2212	40	-0.32396558541029563
2212	41	-0.24932346427222996
2212	42	-0.2026101031675367
2212	43	-0.2839393480814074
2212	44	-0.14367783566764203
2212	45	-0.08662767195405863
2212	46	-0.062294459221703846
2212	47	-0.08748914821076426
2212	48	-0.1787278009190878
2212	49	-0.010293384523212268
2212	50	-0.09126242140988339
2213	1	-0.5574024492685972
2213	2	-0.6366697577920455
2213	3	-0.6812719989626501
2213	4	-0.8417296647922328
2213	5	-0.5997096587664714
2213	6	-0.6276768810218947
2213	7	-0.866049777269783
2213	8	-0.7065209244892827
2213	9	-0.7587558662770135
2213	10	-0.9811717942412734
2213	11	-0.7937521240496643
2213	12	-0.8316726195297761
2213	13	-0.7663091733253609
2213	14	-0.8398586497454943
2213	15	-1.1439710894073087
2213	16	-0.854375637437883
2213	17	-0.9280952386829497
2213	18	-0.8017835072730222
2213	19	-0.9584740821323408
2213	20	-0.8918848203897412
2213	21	-1.0583341085330034
2213	22	-0.8638184157076836
2213	23	-0.9618964649086195
2213	24	-0.9650859768768072
2213	25	-1.0396105038622778
2213	26	-1.045836587277556
2213	27	-1.2803878891919354
2213	28	-1.2335828442180699
2213	29	-1.2178475819025358
2213	30	-1.38251036848078
2213	31	-1.0977795452813162
2213	32	-1.2251835454960904
2213	33	-1.1525010435408838
2213	34	-1.0894013230275448
2213	35	-1.266416684860765
2213	36	-1.4561496413962622
2213	37	-1.1593594962834217
2213	38	-1.0888786841807345
2213	39	-1.1730570102176614
2213	40	-1.0465617645934309
2213	41	-1.0518913167096464
2213	42	-1.0058077316260352
2213	43	-0.9477140516387195
2213	44	-0.9364347526636898
2213	45	-0.9721816903993812
2213	46	-1.0604288465734832
2213	47	-1.095878476274006
2213	48	-1.1121480907741592
2213	49	-0.978009458304502
2213	50	-0.9769738838604738
2214	1	-0.24912733632064032
2214	2	-0.4296634512266877
2214	3	-0.38136356356595513
2214	4	-0.4318927143626148
2214	5	-0.23110620477661925
2214	6	-0.2907293756242737
2214	7	-0.29479710282794613
2214	8	-0.31399449686126246
2214	9	-0.5189383619028689
2214	10	-0.42059197037672647
2214	11	-0.49681524331393234
2214	12	-0.41714510019338036
2214	13	-0.3707624540552459
2214	14	-0.521178191325447
2214	15	-0.43665962841882333
2214	16	-0.3901787375972532
2214	17	-0.251878276264037
2214	18	-0.24982373234039557
2214	19	-0.35817977168751725
2214	20	-0.33407252599887727
2214	21	-0.37511443390108923
2214	22	-0.44318744238143637
2214	23	-0.4135557763525526
2214	24	-0.7210413313652353
2214	25	-0.6949110770091735
2214	26	-0.594750544574211
2214	27	-0.5597728163057809
2214	28	-0.4302270593820978
2214	29	-0.4376291305688511
2214	30	-0.3632630989701511
2214	31	-0.4052673732202763
2214	32	-0.3655162606820497
2214	33	-0.4100229873666379
2214	34	-0.40877951225374787
2214	35	-0.5323355231660827
2214	36	-0.449575022641298
2214	37	-0.36741913085353417
2214	38	-0.39255570664360917
2214	39	-0.3839165843539192
2214	40	-0.40004883467233654
2214	41	-0.26707812555833144
2214	42	-0.23041954157130462
2214	43	-0.3322881878975714
2214	44	-0.16021858222071828
2214	45	-0.1388914689872749
2214	46	-0.10440812360910456
2214	47	-0.09066368021390403
2214	48	-0.08763606979776384
2214	49	0.07298995441487577
2214	50	-0.06002377103436274
2215	1	-0.17404420295477863
2215	2	-0.3085788728266863
2215	3	-0.3057063050917428
2215	4	-0.34572998244529884
2215	5	-0.2453895267997197
2215	6	-0.3534568399280431
2215	7	-0.16901566173253002
2215	8	-0.28436502518932727
2215	9	-0.2319558992485966
2215	10	-0.1875930102493797
2215	11	-0.22302003425436823
2215	12	-0.22425562147188316
2215	13	-0.3073012460719369
2215	14	-0.2040104456915941
2215	15	-0.09064181385734571
2215	16	-0.18473960150689805
2215	17	-0.06996904999702735
2215	18	-0.09566662501776335
2215	19	-0.23174242056554561
2215	20	-0.2052385130061154
2215	21	-0.0347863158197228
2215	22	-0.09072574143074519
2215	23	-0.18404779040650965
2215	24	-0.3180405022267294
2215	25	-0.3175757851862855
2215	26	-0.27893689125947674
2215	27	-0.1818890512392315
2215	28	-0.0904529964904981
2215	29	-0.2153463153111943
2215	30	-0.13089471059715407
2215	31	-0.10057258023521598
2215	32	-0.16263077085687172
2215	33	-0.2350772702245436
2215	34	-0.3718416424498159
2215	35	-0.32033678506221364
2215	36	-0.25332916362996727
2215	37	-0.2942203119682425
2215	38	-0.36552459952418204
2215	39	-0.2746728188859713
2215	40	-0.3320853794853278
2215	41	-0.2530767858912668
2215	42	-0.1881611990706169
2215	43	-0.31263258770038505
2215	44	-0.2999924569568293
2215	45	-0.22225632881771062
2215	46	-0.14206769514143147
2215	47	-0.24302045312152032
2215	48	-0.2818532363517434
2215	49	-0.27862360432036937
2215	50	-0.27700273399658215
2216	1	0.17292205966601415
2216	2	0.18021770670246084
2216	3	0.11194044035865147
2216	4	0.2790247048255843
2216	5	0.21178153436838087
2216	6	0.17800850649519973
2216	7	0.2421828006168667
2216	8	0.32195485663156376
2216	9	0.40408660204982993
2216	10	0.2684149143055947
2216	11	0.053104606589506886
2216	12	0.20669585610105656
2216	13	0.16959777598256665
2216	14	0.09684106771344148
2216	15	0.04185132301248018
2216	16	0.031023339102191017
2216	17	0.15007132528705114
2216	18	0.20530725904448133
2216	19	0.009408475465455979
2216	20	-0.05512742998711673
2216	21	-0.04423241629236685
2216	22	0.08340990151980776
2216	23	-0.046253946986801964
2216	24	-0.1359021523528412
2216	25	-0.32850404749833473
2216	26	-0.13999283399000717
2216	27	-0.15786106281943546
2216	28	-0.1728402721926443
2216	29	-0.3141698515489111
2216	30	-0.2217315395233257
2216	31	-0.4498644128026186
2216	32	-0.25798291879596036
2216	33	-0.38115829718160654
2216	34	-0.406386474592793
2216	35	-0.3190794089774478
2216	36	-0.49898166472092953
2216	37	-0.6579768485129414
2216	38	-0.5818949979215482
2216	39	-0.6756945611276528
2216	40	-0.6592954621242022
2216	41	-0.7753223120657537
2216	42	-0.6079001135373971
2216	43	-0.6708595001726607
2216	44	-0.9056135425463125
2216	45	-0.9174478125232461
2216	46	-0.9897635224778405
2216	47	-0.7440256155457128
2216	48	-0.8761924857906145
2216	49	-0.7451826574809246
2216	50	-1.0282689889867918
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	172152.0
3	8372.0
4	14996.0
5	11578.0
6	9324.0
7	7963.0
8	7048.0
9	6340.0
10	6121.0
11	6132.0
12	6190.0
13	6644.0
14	7025.0
15	7202.0
16	7944.0
17	8419.0
18	9101.0
19	10494.0
20	13552.0
21	18021.0
22	22202.0
23	24298.0
24	25116.0
25	26042.0
26	28488.0
27	32038.0
28	36585.0
29	41366.0
30	48309.0
31	57670.0
32	69284.0
33	85753.0
34	107784.0
35	139077.0
36	180201.0
37	242664.0
38	370993.0
39	556477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.265112889386415	17.337787547759632	12.845314806296248	24.551784756557705
2	16.24057404211749	19.27620954917503	38.91679713389323	25.56641927481425
3	20.106363855004346	23.839523390115993	27.51670194890956	28.537410805970097
4	13.0788031265968	15.56325325310946	35.7016065979483	35.65633702234544
5	14.864099544057355	37.239761809981495	32.82335608258677	15.072782563374387
6	33.909532516431234	35.284583130092386	16.737440325108928	14.068444028367445
7	29.906619884307606	31.301393830422562	20.215115165254048	18.576871120015788
8	26.93684080541891	34.90311334505827	18.97709123833506	19.18295461118776
9	26.753705809665874	16.44488423498599	18.32966567713795	38.47174427821018
10	15.184892428945348	29.165950538111645	32.29092361919818	23.358233413744827
11	36.06451395890882	23.259679432012003	21.95706945366269	18.718737155416488
12	24.14620378210556	24.756726332302804	28.621290589370457	22.475779296221177
13	28.521118382891654	21.64748081620062	24.899394273534355	24.93200652737337
14	22.585476554404462	21.06259788996614	25.836141324190237	30.51578423143916
15	24.26331565692926	28.822999262013205	22.59183094974675	24.321854131310783
16	24.353080656211155	27.882697538182576	24.16580859277249	23.598413212833776
17	23.33095534733022	27.818990276238832	25.47688601707309	23.373168359357855
18	23.20786111687721	27.18272510294886	26.373656577855925	23.235757202318
19	24.313583103584087	26.845419784692137	26.164664566102143	22.676332545621637
20	24.265805506104833	26.282642776052985	26.253225351829524	23.198326366012658
21	24.62920820883335	26.029940724193935	26.203796179201195	23.137054887771523
22	24.32019098829384	25.76704713320596	26.10295083151143	23.809811046988766
23	24.011744565143815	25.862206893552674	26.224783625217583	23.901264916085925
24	23.665122241042003	26.052950328908498	26.142440028360976	24.139487401688527
25	24.027376664262036	25.938769765355772	26.219733882212555	23.814119688169633
26	23.61352009989114	26.28014245073866	26.479317437905536	23.627020011464666
27	23.877767063988674	26.179595018771927	26.058387871078924	23.884250046160474
28	23.861209913296207	26.27867264035143	26.055418689392607	23.80469875695976
29	23.899412877948535	26.27683042726794	26.134850954381093	23.688905740402433
30	23.847344757815502	26.288413805724716	26.244015810938404	23.62022562552138
31	23.976761005682988	25.82634668880842	26.0946404023257	24.102251903182893
32	23.57918616075366	26.326360244574303	26.03809730873916	24.056356285932875
33	23.864071305296005	26.09927425438879	25.965291711501866	24.071362728813344
34	24.22282104178469	26.112917642802262	25.94735574763543	23.71690556777762
35	24.213696377760492	26.114576256723804	25.899734493815245	23.771992871700455
36	23.994671834565963	26.277848976785478	25.743514554408165	23.983964634240394
37	23.993614863534322	25.850777178265265	26.162649495227967	23.992958462972442
38	24.12525551026369	26.169829873134	26.055216880680653	23.649697735921656
39	24.28164361712602	26.087482688230622	25.839210583316373	23.791663111326983
40	24.236825123594272	26.026116532854065	26.240588641429134	23.49646970212253
41	23.819895648749753	26.156026448776004	26.074030320929314	23.950047581544922
42	24.30044351764083	25.99067517798335	25.960486610180684	23.74839469419514
43	23.856261694969756	25.930121812261948	26.1253626927491	24.088253800019196
44	24.129668405067132	26.036098299266015	25.888686314761912	23.94554698090494
45	24.045975597252127	26.11061211934789	25.824423254383266	24.018989029016712
46	24.064093704438125	26.05510795409106	25.75945175029375	24.12134659117707
47	23.9541939085166	26.162552482557764	25.777430890360925	24.105822718564706
48	23.983188342079835	26.24951400725421	25.867240725946022	23.90005692471993
49	24.14897870883205	25.98941490812378	25.775621479411154	24.085984903633012
50	24.18431598197262	26.098730627312328	25.83629271485643	23.88066067585862
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2837.0
1	2094.5
2	1352.0
3	1240.0
4	1128.0
5	1084.0
6	1040.0
7	1008.5
8	977.0
9	1068.5
10	1160.0
11	1441.5
12	1723.0
13	2815.5
14	3908.0
15	5316.0
16	6724.0
17	7347.5
18	7971.0
19	8642.5
20	9314.0
21	10823.0
22	12332.0
23	15638.0
24	18944.0
25	23628.5
26	28313.0
27	33213.5
28	38114.0
29	43588.5
30	49063.0
31	54151.5
32	59240.0
33	66648.5
34	74057.0
35	82007.0
36	89957.0
37	95821.5
38	101686.0
39	109129.0
40	116572.0
41	123790.5
42	131009.0
43	135279.5
44	139550.0
45	146195.5
46	152841.0
47	159754.0
48	166667.0
49	167297.5
50	167928.0
51	169074.5
52	170221.0
53	165681.0
54	161141.0
55	157646.5
56	154152.0
57	147532.5
58	140913.0
59	129537.5
60	118162.0
61	105367.5
62	92573.0
63	81333.0
64	70093.0
65	60328.5
66	50564.0
67	43322.0
68	36080.0
69	29719.5
70	23359.0
71	19165.0
72	14971.0
73	12556.5
74	10142.0
75	7550.5
76	4959.0
77	3899.0
78	2839.0
79	2333.0
80	1827.0
81	1411.0
82	995.0
83	843.5
84	692.0
85	520.0
86	348.0
87	298.0
88	248.0
89	186.0
90	124.0
91	89.5
92	55.0
93	44.0
94	33.0
95	27.0
96	21.0
97	15.5
98	10.0
99	23.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08290401871285566
2	0.09061220640722602
3	0.09934541906095413
4	0.1004524460170605
5	0.09245725133406998
6	0.05666337975329699
7	0.05305529189635768
8	0.05846742368176665
9	0.05908243865738131
10	0.04460908623124973
11	0.04784816510282026
12	0.05662237875492268
13	0.050472228998776125
14	0.05244027692074302
15	0.05133324996463664
16	0.0547363328297044
17	0.05444932584108422
18	0.05522834481019613
19	0.06252652252082339
20	0.06699563134362321
21	0.0772048799388265
22	0.08023895381852549
23	0.057893409704526305
24	0.07433481005262478
25	0.08196099575024653
26	0.0783939088916815
27	0.07441681204937341
28	0.09340027429667913
29	0.08171498976000066
30	0.07863991488192737
31	0.05485933582482734
32	0.051661257951631116
33	0.054695331831330095
34	0.04944720403941836
35	0.055638354793939235
36	0.05547435080044199
37	0.05859042667688959
38	0.049037194055675255
39	0.05399831485896682
40	0.05543334980206768
41	0.04284604330115438
42	0.03919695444584075
43	0.03899194945396921
44	0.03690089853687937
45	0.029315713837631947
46	0.027060658927044873
47	0.031734772741716266
48	0.02693765593192194
49	0.025830628975815558
50	0.02763467290428522
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2438965.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.24907923588381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.39664552584405	51.48262882399257
2	10.767935327292836	13.621239894456341
3	3.3605659100067244	6.376580985582758
4	1.5025592650095365	3.8014196003679808
5	0.7879816609737886	2.4919557255677245
6	0.4858810662085095	1.8438918033502634
7	0.32611643517067684	1.4438594973763874
8	0.21817889965248335	1.103969160937429
9	0.16501663242582848	0.9393435053585945
>10	0.8978144664802016	10.178552235558232
>50	0.05933427400784978	2.563353994093476
>100	0.03105496136517831	3.600584488371016
>500	6.539825446675239E-4	0.2843347184886043
>1k	2.615930178661367E-4	0.26828556649874036
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	4.1000998374310416E-5
11	0.0	0.0	0.0	0.0	4.1000998374310416E-5
12	0.0	0.0	0.0	0.0	2.870069886201729E-4
13	0.0	4.1000998374310416E-5	0.0	0.0	3.2800798699448333E-4
14	0.0	4.1000998374310416E-5	0.0	0.0	3.2800798699448333E-4
15	0.0	4.1000998374310416E-5	0.0	0.0	4.92011980491725E-4
16	0.0	4.1000998374310416E-5	0.0	0.0	5.330129788660353E-4
17	0.0	4.1000998374310416E-5	0.0	0.0	5.330129788660353E-4
18	0.0	4.1000998374310416E-5	0.0	0.0	5.330129788660353E-4
19	0.0	4.1000998374310416E-5	0.0	0.0	5.740139772403458E-4
20	0.0	4.1000998374310416E-5	0.0	0.0	6.560159739889667E-4
21	0.0	4.1000998374310416E-5	0.0	8.200199674862083E-5	6.560159739889667E-4
22	0.0	4.1000998374310416E-5	0.0	8.200199674862083E-5	6.560159739889667E-4
23	0.0	4.1000998374310416E-5	0.0	1.6400399349724166E-4	6.560159739889667E-4
24	0.0	4.1000998374310416E-5	0.0	2.0500499187155207E-4	6.560159739889667E-4
25	0.0	8.200199674862083E-5	0.0	2.870069886201729E-4	6.560159739889667E-4
26	0.0	8.200199674862083E-5	0.0	4.510109821174146E-4	7.380179707375874E-4
27	0.0	8.200199674862083E-5	0.0	4.92011980491725E-4	7.380179707375874E-4
28	0.0	8.200199674862083E-5	0.0	0.0012710309496036228	7.790189691118979E-4
29	0.0	8.200199674862083E-5	0.0	0.0026240638959558666	7.790189691118979E-4
30	0.0	8.200199674862083E-5	0.0	0.004715114813045698	7.790189691118979E-4
31	0.0	8.200199674862083E-5	0.0	0.010168247596828982	8.200199674862083E-4
32	0.0	8.200199674862083E-5	0.0	0.015580379382237957	8.200199674862083E-4
33	0.0	8.200199674862083E-5	0.0	0.023575574065228488	8.200199674862083E-4
34	0.0	8.200199674862083E-5	0.0	0.03333381167831437	9.020219642348292E-4
35	0.0	8.200199674862083E-5	0.0	0.04522410120686439	9.020219642348292E-4
36	0.0	8.200199674862083E-5	0.0	0.06027146761023631	9.020219642348292E-4
37	0.0	8.200199674862083E-5	0.0	0.08015695182177686	9.020219642348292E-4
38	0.0	8.200199674862083E-5	0.0	0.10434754086262	9.020219642348292E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1980	0.0	25.45229	1
GTATCAA	3870	0.0	21.836187	1
CGTCGCG	65	4.9402716E-4	20.303953	32
CGTAGAC	110	2.7900187E-6	18.00592	3
TAGCGCG	140	8.374445E-8	17.287142	29
ATCAACG	5050	0.0	16.516321	3
TCAACGC	5095	0.0	16.283724	4
TATCAAC	5165	0.0	16.276405	2
CAACGCA	5175	0.0	16.114729	5
AACGCAG	5320	0.0	15.713326	6
CGAACGT	200	1.2732926E-9	15.404748	4
TATACTG	505	0.0	15.250035	5
GGACCGT	190	1.0786607E-8	15.051713	6
GTACATG	7950	0.0	14.560463	1
GTATAGA	410	0.0	14.492271	1
CTTACAC	475	0.0	14.362616	3
CGACAGG	460	0.0	14.344891	34
GTGTACG	280	3.6379788E-12	14.147219	1
TACATGG	7905	0.0	14.114697	2
GTCGGTT	125	1.6735715E-4	14.078562	12
>>END_MODULE