FastQCFastQC Report
Mon 6 Feb 2017
SRR2077088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438965
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35270.14461052126619284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26100.025.2906931
TGCGATA1254.0017767E-1122.88442613
GTATCAA53850.022.6770861
GCGATAC1452.9649527E-1019.72714414
TAGATCG600.007405065518.3372555
GTACCGA2301.8189894E-1216.2640156
TATCAAC76750.015.7388832
GCACCGT2850.015.4415846
ATCAACG78100.015.3823123
TCAACGC79150.015.206364
CAACGCA80450.014.9342095
CGATACT1951.5150363E-814.66890215
AACGCAG82600.014.5718266
TAGGCCG3800.014.47381323
TCTATAC2803.6379788E-1214.1447233
TAGGACA4850.014.0639984
CGATCGT1100.001018452914.00012425
GTACATG82250.013.9106491
TACGAGA1751.052058E-613.830684
GTGCCGA2256.708433E-913.69181711
Produced by FastQC (version 0.11.5)