FastQCFastQC Report
Wed 25 May 2016
SRR2074918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177839
Sequences flagged as poor quality0
Sequence length60
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3400.035.7352941
AACGTTC350.004474677630.85714324
GTATCAA4100.028.3170722
GGACTTC400.00859114327.06
CAAGACT400.00859114327.04
AAGACTG1052.4804285E-723.1428575
TATCAAC4950.022.909093
TCAACGC5100.022.7647065
ATACTCT600.002257145522.56
CAACGCA5200.022.3269236
ATCAACG5300.021.905664
GGGGCAC650.003591523320.7692321
GGGGGGT650.003591523320.7692323
AACGCAG5750.020.1913057
TTCTCTA700.00551487819.28571528
TGGACTT750.0082123318.0000025
TGGGGGG750.0082123318.0000025
AATAGGA750.0082123318.0000022
GAATTAA750.0082123318.00000224
ACGCAGA7800.014.8846168
Produced by FastQC (version 0.11.5)