FastQCFastQC Report
Wed 25 May 2016
SRR2074917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52891
Sequences flagged as poor quality0
Sequence length60
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATAGCAGAGTACACTAACATTATTCTAATAAACGCCCTAACAACTATTATCTTCCT690.1304569775576185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.10209676504509273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.10209676504509273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCA150.006186416454.020
GGCGGCT150.006186416454.039
GTCTTTG150.006186416454.034
TCCCAGA150.006186416454.035
TTCGAGG150.006186416454.013
TAATCTT150.006186416454.02
CAGTTAA258.524893E-443.22
AGTTAAG258.524893E-443.23
TTAAGAG452.0340303E-742.05
GTTAAGA404.3888067E-640.54
ACTGACT300.0020890136.013
GTGGTAT502.0265898E-532.41
CCTTAAA350.00444654430.8571439
GCACTGA350.00444654430.85714311
GCACCCG454.1725743E-430.09
ACCCGAC454.1725743E-430.011
CTTAAAG400.00853755627.010
GAAGAAT400.00853755627.01
AGATAGA400.00853755627.041
CTGACTG507.728856E-427.014
Produced by FastQC (version 0.11.5)