FastQCFastQC Report
Wed 25 May 2016
SRR2074916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76824
Sequences flagged as poor quality0
Sequence length60
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2650.34494428824325735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCC202.844734E-454.032
AAGGTAG150.006197075454.030
ACCCTTA202.844734E-454.035
ATTGGCG150.006197075454.021
TGAGGCG150.006197075454.012
CCGCGTC150.006197075454.023
CGCGTCC150.006197075454.024
GCGTCCC150.006197075454.025
CCCGCGT150.006197075454.022
TTGATCA258.54918E-443.19999723
CCTAATG258.54918E-443.19999749
GACCCTT258.54918E-443.19999734
TGGCAGA258.54918E-443.19999754
AGCGGAT258.54918E-443.19999735
CAGCTGG258.54918E-443.19999728
TTCCTAA258.54918E-443.19999747
GCGGATG300.002094914736.036
TTGCCAT300.002094914736.014
GGACCCT300.002094914736.033
GCAGCGT300.002094914736.01
Produced by FastQC (version 0.11.5)