FastQCFastQC Report
Wed 25 May 2016
SRR2074915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132615
Sequences flagged as poor quality0
Sequence length60
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTC150.006208011453.99773815
GCGCTTT150.006208011453.99773842
TTAGCAG359.613277E-538.56981321
GAGTATT359.613277E-538.56981316
AGGTTAG359.613277E-538.56981326
TGTTGTA300.002100839535.9984919
TTATAAC300.002100839535.9984935
TCTGGCC300.002100839535.9984945
ATATGGG402.108442E-433.7485936
TAGCAGG402.108442E-433.7485922
TCGTGTG402.108442E-433.7485912
CAGCTCG402.108442E-433.748598
TAGTGTC402.108442E-433.7485930
GCTCGTG402.108442E-433.7485910
GGTTAGT402.108442E-433.7485927
TACTTAT350.00447153130.85584815
CTGCCTG350.00447153130.8558489
GGTATCA4300.030.7777581
CTCTTTA454.207437E-429.99874348
GTTAGTG454.207437E-429.99874328
Produced by FastQC (version 0.11.5)