FastQCFastQC Report
Wed 25 May 2016
SRR2074914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593088
Sequences flagged as poor quality0
Sequence length60
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7280.12274738318765512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG150.006217887653.99932548
CGGCATA1200.040.49949628
CCGTCTT904.7293724E-1132.99958447
TCTCGTA1150.032.86915638
GGTATCA11800.032.262311
TAGCGTC859.222276E-1031.76430936
GGATAGG454.2241995E-429.9996241
CTCGTAT1101.4551915E-1129.45417839
ACGGCAT1500.028.7996427
GCGTCGT753.453897E-728.7996438
CGTGGTA853.232708E-828.5878842
TGGGCGA400.00860757826.99966239
GGTATTC1058.809366E-925.71396645
GGCATAG1800.025.4996829
CGTCGTG851.0306285E-625.41144839
AGACGGC1600.025.31218325
GACGGCA1750.024.68540626
TATGCCG1001.5614387E-724.29969643
GTAGCGT1001.5614387E-724.29969635
TCGTGGT1001.5614387E-724.29969641
Produced by FastQC (version 0.11.5)