FastQCFastQC Report
Wed 25 May 2016
SRR2074913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95709
Sequences flagged as poor quality0
Sequence length60
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGTGATTTTCTTGCCATTCTTGATCAGCTGGACCCTTACACACTTCCTAATGGCAGA2140.22359443730474668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAA150.00620314853.99686442
GGACCCT408.1545295E-847.24725733
GCATGAC258.540201E-443.220071
CTAATGG452.0583138E-741.99756250
GACCCTT404.4282315E-640.4976534
TGGACCC559.919804E-734.3616432
CCTAATG559.919804E-734.3616449
ACCCTTA559.919804E-734.3616435
TAATGGC659.707037E-833.2288451
CCCTTAC502.0446001E-532.39811736
CACACTT502.0446001E-532.39811742
CTGGACC601.957529E-631.4981731
ATATAAC350.004454307730.871483
AGAATAC350.004454307730.871483
GGCCCTT350.00446572330.85535210
TACTGTC350.00446572330.8553527
CCTTACA553.920702E-529.45283537
CTTCCTA553.920702E-529.45283546
ACACTTC553.920702E-529.45283543
TCTTGCC653.6534493E-629.07523512
Produced by FastQC (version 0.11.5)