FastQCFastQC Report
Wed 25 May 2016
SRR2074912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9492
Sequences flagged as poor quality0
Sequence length60
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210.22123893805309736No Hit
GTAATCTTCCTTAAAGAGTATTAGCAGGTTAGTGTCTTTGTACTTCTGGCCAGGGATCTC150.15802781289506954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTTC150.0060306954.0000043
GGTATCA202.7184782E-454.01
GTATCAA300.002003342836.0000042
TATCAAC350.004265592430.8571433
ACGCAGA400.00819278427.08
ATCAACG400.00819278427.04
Produced by FastQC (version 0.11.5)