FastQCFastQC Report
Wed 25 May 2016
SRR2074911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26923
Sequences flagged as poor quality0
Sequence length60
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTAGCTCTCAGCAGCCCGCTCCTGAGCTCTGAGGAAGCTTGCCTTCTTTTGAGCTA510.1894291126546076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.15228614938899826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTGT150.00615351254.00000433
GGTATCA654.7293724E-1141.5384641
GTATCAA1004.778485E-926.9999982
TATCAAC1251.2428991E-619.443
ATCAACG1452.4516703E-718.6206874
TCAACGC1554.939193E-717.4193555
AACGCAG1606.888531E-716.8757
CAACGCA1606.888531E-716.8756
AGTACTT1307.313766E-414.53846212
ACGCAGA1904.12955E-614.2105268
GTACTTT1359.7331917E-414.013
ACTTTTT1359.7331917E-414.015
GAGTACT1359.7331917E-414.011
TACTTTT1359.7331917E-414.014
GCAGAGT1753.0493085E-513.88571510
CGCAGAG1955.405018E-613.8461549
CAGAGTA1803.975569E-513.511
CTTTTTT1400.001281154913.516
AGAGTAC1754.8085884E-412.34285712
Produced by FastQC (version 0.11.5)