FastQCFastQC Report
Wed 25 May 2016
SRR2074910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88438
Sequences flagged as poor quality0
Sequence length60
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACT150.00620016553.9999964
GGTTAGA258.55622E-443.22
ACAACGC350.00446262930.8571434
AACAACG350.00446262930.8571433
CCTCGAA350.00446262930.8571432
TCAGAGT350.00446262930.8571438
GGGTTAG350.00446262930.8571431
AGGGGCA350.00446262930.8571434
GCACCCG706.4922096E-627.09
GTGTGCT507.770445E-427.011
TCTAAAT400.00856819227.013
ACCCGAC706.4922096E-627.011
CGACTGC805.973943E-727.014
CCCGACT751.1091812E-525.19999912
GAAGGTC901.6682861E-624.027
GAGTAAA702.0183943E-423.1428591
CGAAGGT702.0183943E-423.14285926
CTCACAA600.00224823922.49999812
GGGTAAG600.00224823922.4999981
CCGAAGG600.00224823922.49999825
Produced by FastQC (version 0.11.5)