FastQCFastQC Report
Wed 25 May 2016
SRR2074909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3368717
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT8000.038.13675735
CGTCGTG8300.037.7347639
GCGTCGT8400.036.6426938
TAGCGTC8650.036.20738636
CGTGGTA9050.033.71204842
AGTAGCG9300.033.3859534
AGCGTCG9650.033.014937
GTCGTGG11150.029.54246540
TCGTGGT11100.029.43229541
GGGTAGT12900.025.53476524
GGTATTC10050.023.37233245
GTGTAAG14050.023.0603912
GTATTCC10600.022.41432646
GGTGTAA14600.022.37660611
GGTAGTC14650.022.29990625
TAAGCAT14850.022.18237315
TATGCCG2800.022.17813943
GTAGTCT15000.021.95957426
TGAGTAG15550.020.31441532
TGTAAGC15950.020.31398613
Produced by FastQC (version 0.11.5)