FastQCFastQC Report
Wed 25 May 2016
SRR2074906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988552
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4750.034.10470641
TATGCCG5100.030.70537843
TCGTATG5150.030.40726740
TAGCGAT5200.028.55722432
ATAGCGA6350.028.06253231
TCTCGTA5150.027.78595238
CTCGTAT5650.026.76062239
ATTGGCG4200.025.71429321
CATAGCG7150.025.677930
GGTAGCG4750.025.01011732
GGTATCA60750.024.8012521
ATTTACG4350.024.206554
GCCGTCT6050.024.09877846
ATGCCGT6600.023.72688144
AGCGGAT5050.023.52436635
AGCGATC6000.023.39961433
CCGTCTT6400.023.20274247
TAGCGGA5050.022.45507834
ATCTCGT6300.021.85678537
TGCCGTC7050.021.8294345
Produced by FastQC (version 0.11.5)