FastQCFastQC Report
Wed 25 May 2016
SRR2074903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3817692
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40500.10608503776627345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73500.026.5234011
GACGTTA5050.025.66294326
CGTTAGG5650.023.89341228
ACGTTAG5250.023.65675427
TATGCCG4550.023.14247543
TCGTATG4850.022.26767340
TCTCGTA4500.022.19963538
GTATCAA118150.021.6190591
CGAGACG6250.020.30366523
CGTATGC5550.019.94561841
CTCGTAT5250.019.02825739
GTTAGGT7350.017.2650229
CAACGCA163600.016.850855
ATCAACG154800.016.762233
TCAACGC155600.016.6413424
TATCAAC152300.016.629622
AACGCAG168100.016.5284716
ACAACGC9500.016.4848024
ACGAGAC7900.016.40500822
AACAACG10600.015.5382933
Produced by FastQC (version 0.11.5)