FastQCFastQC Report
Wed 25 May 2016
SRR2074902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626573
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6510.10389850823447548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG1000.040.539
TAGCGTC1000.037.80000336
GTAGCGT1000.037.80000335
AGCGTCG1100.036.81818437
AGTAGCG1100.034.36363634
GCGTCGT1100.034.36363638
TCGTGGT1201.8189894E-1229.24999841
GTCGTGG1550.027.87096840
ATGCCGT1500.027.00000244
CGTGGTA1104.8930815E-1027.042
TATGCCG1303.6379788E-1227.043
ATATCTA2150.026.3720931
CGGCATA1850.026.2702728
TATCTAA2250.025.1999992
CGTATAG651.2326232E-424.92307728
TCGTATG1950.024.92307740
GGGTAGT1603.6379788E-1223.62524
CTCGTAT1653.6379788E-1222.90909239
ACGTATA600.002263469622.49999827
AGACGGC2050.022.39024525
Produced by FastQC (version 0.11.5)