FastQCFastQC Report
Wed 25 May 2016
SRR2074901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313751
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3600.039.74933239
CGTGGTA4000.036.44938742
GCGTCGT4000.036.44938738
ATTGGCG2900.034.44769721
AGCGTCG4400.033.13580737
GTAGCGT4200.031.49947435
TAGCGTC4450.030.94329836
ATTTACG3300.027.81891854
AGTAGCG5100.026.47014434
TAGCGGA3550.025.8587234
GTCGTGG5750.025.82565340
TCGTGGT5600.025.5531441
TATGCCG3450.024.26046243
GGGTAGT6400.023.20273624
AGCGGAT4000.022.94961535
CTCGTAT3550.022.81651939
TCGTATG3550.022.81651940
TTGGCGT4400.022.70416522
GGTATCA55450.022.3022441
TCTCGTA3600.021.74963438
Produced by FastQC (version 0.11.5)