FastQCFastQC Report
Wed 25 May 2016
SRR2074900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959862
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC47160.24062918715705492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23120.11796748954773345No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.10643606539644118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG9750.044.03037339
AGCGTCG10050.043.7906537
GCGTCGT10000.042.92961538
GTAGCGT10200.042.61726835
TAGCGTC10300.042.46563736
AGTAGCG10550.041.97118834
CGTGGTA10250.041.61913742
TCGTGGT10900.039.38496841
GGTATTC8850.038.44033445
GTCGTGG11300.038.22974840
GGGTAGT11700.036.4621424
GTGTAAG12250.036.3670212
TAAGCAT12000.036.22652415
GTATTCC9550.035.62271546
TGGTGTA12800.034.1715710
TGTAAGC12950.033.56812313
CTGGGTA12900.033.48892622
GGTGTAA13550.033.07719411
TGGGTAG13400.032.44083423
AAGCATC13750.032.2049916
Produced by FastQC (version 0.11.5)