FastQCFastQC Report
Wed 25 May 2016
SRR2074898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610548
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAATTTACG19080.11846899316257573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA4500.040.79899234
ATTGGCG4000.040.49899721
TATGCCG2050.038.1941843
ATTTACG4150.037.73400554
GGTAGCG5050.036.35553732
AGCGGAT5050.035.82089635
TTGGCGT4300.035.16192222
GTAGCGG5700.032.2097333
TCGTATG2300.031.69486840
CGTATGC2700.029.99925641
GAGGCGC6200.029.17669713
CGGATGA6200.028.74122437
AGGTAGC6850.027.98470931
GCGGATG6400.027.84306336
GGCGCCA6400.026.15641615
GCGTGAA6300.025.71444725
GCGCCAT6200.025.6937116
TGAGGCG6850.025.62059812
CTCGTAT2850.025.57831439
ATGCCGT2800.025.0708144
Produced by FastQC (version 0.11.5)