FastQCFastQC Report
Wed 25 May 2016
SRR2074897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2076194
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG4100.040.17017721
TCGTATG4150.035.13204240
CGTATGC4050.034.66618741
TAGCGGA4900.034.16279234
AGCGGAT4900.034.16279235
TATGCCG3800.034.10479443
TTGGCGT5000.031.85955822
ATTTACG4900.031.40848554
GGTAGCG5750.029.582232
TCTCGTA4950.029.4541438
TATAACG1300.029.078622
CTCGTAT4950.028.90869139
GAGGCGC6300.028.28532213
GTAGCGG6250.027.6476233
ATCTCGT5250.026.2282137
CGGATGA6500.026.16886937
GCGGATG6600.025.77237136
ATGCCGT4850.025.6085144
ACGAGAC7050.024.12732722
GGCGCCA7450.023.91913215
Produced by FastQC (version 0.11.5)