FastQCFastQC Report
Wed 25 May 2016
SRR2074896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930341
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC22380.11593806482896028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC4900.040.22381636
CGTCGTG4850.039.5251139
CGTGGTA4700.037.91425742
GTAGCGT5200.037.9032135
GCGTCGT5050.037.42511438
AGCGTCG5400.035.99939337
AGTAGCG6150.032.92627734
GGGTAGT6500.029.49181224
GTCGTGG6650.028.82658440
TCGTGGT6600.027.81771541
TCGTACG507.8304886E-426.99954427
ATTGGCG2700.026.00023721
TAAGCAT8000.025.98706215
ATTTACG2600.025.96110254
TAGCGGA2850.025.57851634
TGGGTAG7850.024.76455723
GTGTAAG8400.024.75022512
GGTAGCG3100.024.38668632
GGTATTC4800.024.18709445
GGTAGTC8150.024.18364325
Produced by FastQC (version 0.11.5)