FastQCFastQC Report
Wed 25 May 2016
SRR2074895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847134
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6700.028.20845843
CTCGTAT7250.026.06850639
GGTATCA111200.025.6913131
TCTCGTA8500.023.18782838
ATGCCGT9000.020.99963244
GTATCAA173450.020.845471
CCGTCTT8200.020.41427647
TCGTATG10450.019.63601740
GCCGTCT8950.019.0052546
CGAGACC10700.017.91557323
TGCCGTC10850.017.41904645
ATCAACG221250.016.7202473
CCCACGA13150.016.63088619
TCAACGC222750.016.5834084
CAACGCA227700.016.4365275
TATCAAC220300.016.314322
CGATCGT3700.016.05377225
AACGCAG234650.016.0187476
ACGAGAC14450.015.50837822
CGTATGC12950.015.2198141
Produced by FastQC (version 0.11.5)