FastQCFastQC Report
Wed 25 May 2016
SRR2074894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415612
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC25010.10353483920430931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC5550.037.45825236
GTAGCGT5800.036.7746835
CGTCGTG5900.036.6089939
GCGTCGT5900.036.6089938
CGTGGTA6150.035.9988442
AGCGTCG6250.034.9908737
AGTAGCG6950.032.24356534
TATGCCG4200.031.49898543
ATAGCGA4550.030.85615231
TAGCGAT4050.030.6656832
TCGTGGT7850.028.20291341
CTCGTAT4150.027.97500239
CGATCTC4550.026.70243835
GTCGTGG8400.026.67771140
GGGTAGT8600.025.74335724
GGTATTC6650.025.57865345
GGTAGTC9250.024.81001325
TAAGCAT9300.024.67866715
GGTATCA57700.024.3822981
GTATTCC7000.023.9140146
Produced by FastQC (version 0.11.5)