FastQCFastQC Report
Wed 25 May 2016
SRR2074893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621852
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT3050.040.7200638
CGTCGTG3450.036.78147539
AGCGTCG3400.035.7341937
ATTTACG2250.034.854
TAGCGTC3400.034.14600836
GTAGCGT3500.033.9418135
ATTGGCG2400.033.75208321
CGTGGTA3850.033.661342
TAGCGGA2550.031.76372734
TCGTGGT4100.030.95026441
TAACGCA1900.029.8421064
TATAACG1850.029.1891882
AGTAGCG4350.028.55084234
GGTAGCG3000.027.8991432
TATCACG1204.5474735E-1127.02
AGCGGAT3100.026.12822535
GGGTAGT5000.025.9224
GTCGTGG5050.025.1279440
TGGGTAG5400.023.523
ATAACGC2200.023.3181823
Produced by FastQC (version 0.11.5)