FastQCFastQC Report
Wed 25 May 2016
SRR2074892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139804
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC28070.1311802389377719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT6550.043.69452335
TAGCGTC6750.042.3998736
CGTCGTG6850.041.78089539
AGCGTCG7100.040.69001437
CGTGGTA7000.039.3427442
GCGTCGT7300.039.20536438
AGTAGCG7500.038.51988634
TCGTGGT7650.036.70577241
GTCGTGG8450.034.18924340
TATGCCG3800.034.1051643
GGTATTC6400.031.6405345
CTCGTAT4200.031.49990539
CCGTCTT3800.031.26306347
GCGATCT4450.029.73024634
GGTAGTC9700.029.22671525
GGGTAGT9850.028.7816424
TCTCGTA4650.028.45152738
CGATCTC4750.028.42096535
CTGGGTA10050.028.2088722
GAGTAGC10600.027.76406733
Produced by FastQC (version 0.11.5)