FastQCFastQC Report
Wed 25 May 2016
SRR2074891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566958
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT6450.037.25522235
CGTCGTG6750.035.19944439
TAGCGTC7000.034.32802636
AGTAGCG7250.034.26152834
AGCGTCG7300.033.65737
ACGTTAG5750.031.92992827
GCGTCGT7850.030.26703838
CGTGGTA7650.029.99952742
TATGCCG5050.029.94011943
GACGTTA6450.028.46466426
GGTATCA67850.028.1356471
TCGTGGT9250.025.39419441
CTCGTAT6000.025.19960239
TCTCGTA6150.024.14595838
GTATCAA112350.022.2789131
CGTTAGG8150.022.19596728
GTTAGGT9000.021.29966229
GGGTAGT11600.021.18099624
GAGTAGC12150.021.11077533
GTCGTGG11400.021.07861340
Produced by FastQC (version 0.11.5)