FastQCFastQC Report
Wed 25 May 2016
SRR2074890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620047
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1800.037.4988243
CGGCATA2450.036.36620728
GCCGTCT1800.035.9988746
CCGTCTT1700.034.9400847
GCGTCGT551.0035037E-634.3625638
CTCGTAT2300.034.0424139
ACGGCAT2550.033.8812927
TCTCGTA2350.033.31810438
GACGGCA2850.033.15685726
TAGCGTC502.0622883E-532.39898336
CGTCGTG502.0622883E-532.39898339
TCGTATG2400.031.49901240
TAGGATC2550.030.7049233
TGCCGTC2200.030.68085545
ATGCCGT2400.029.24908344
CGTATGC2450.028.65216441
CCACGAG2900.027.93241120
AGACGGC3000.027.90137525
ATCTCGT2750.027.49004637
CGAGACG3050.027.44397523
Produced by FastQC (version 0.11.5)