FastQCFastQC Report
Wed 25 May 2016
SRR2074889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947980
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG4450.043.07789221
TAGCGGA5200.036.34551234
TTGGCGT5150.036.1741222
GGTAGCG5300.035.65974832
AGCGGAT6050.032.13166835
TCGTATG2700.031.99943540
ATTTACG5450.031.2104654
GGCGCCA6350.030.61363615
GAGGCGC6850.030.3498313
GTAGCGG6350.029.76325633
GCGTGAA6950.027.97073225
TGAGGCG7550.027.89354912
CGGATGA7100.027.76007337
CGTATGC3050.027.4421441
GCGGATG7200.027.37451736
AAGGTAG7050.027.19101130
CTCGTAT3100.026.99952539
AGGTAGC7500.026.6395331
GCGCCAT7450.026.4559116
TCTCGTA3150.025.71383338
Produced by FastQC (version 0.11.5)