FastQCFastQC Report
Wed 25 May 2016
SRR2074888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737283
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAATTTACG19160.11028715528788344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG4850.041.1951321
GGTAGCG5150.040.36820232
TAGCGGA5450.038.14609534
AGCGGAT5850.036.46087635
ATTTACG5200.036.34549754
GTAGCGG6550.032.1520933
TATGCCG2700.031.9994243
GAGGCGC6500.031.98495713
AGGTAGC6750.031.19943431
CGGATGA7000.030.08516737
TTGGCGT6500.029.90715222
GCGTGAA6950.029.52464525
GGCGCCA7450.028.99443415
GCGGATG7550.028.96636436
TCGTATG2750.028.4722140
AAGGTAG7500.028.43948630
CGTATGC2950.028.37236841
GAAGGTA7700.027.35015329
CGTGAAG8000.027.33700426
GCGCCAT8000.027.00106616
Produced by FastQC (version 0.11.5)