FastQCFastQC Report
Wed 25 May 2016
SRR2074886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603553
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC3550.043.35133436
GTAGCGT4150.037.08367535
GCGTCGT4100.036.8773938
AGCGTCG4300.036.41795337
CGTCGTG4250.035.57583239
CGTGGTA4150.035.131942
ATTGGCG2100.034.7136621
AGTAGCG4700.032.74409534
TCGTGGT5450.028.7334341
TATGCCG2200.028.22676743
TAGCGGA2400.028.12449634
GGGTAGT5250.027.77093124
ATTTACG2400.027.00035954
GGTATTC4250.026.68270745
AGCGGAT2700.025.99953535
GTCGTGG6150.025.02394140
GGTAGCG2700.024.99955432
GTAAGCA5800.024.2072214
GGTAGTC6300.023.14244525
TCGTATG3050.023.01598240
Produced by FastQC (version 0.11.5)