FastQCFastQC Report
Wed 25 May 2016
SRR2074885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702799
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC21470.12608651990046976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17150.10071652614313258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4350.043.447539
GTAGCGT4450.043.0778835
CGTGGTA4750.041.49398842
TAGCGTC4750.039.78875736
GCGTCGT4900.039.12174638
AGTAGCG5050.038.49435834
AGCGTCG5200.037.38394537
TCGTGGT5400.035.49936741
GTCGTGG5850.032.76864240
CGACTTA801.7971615E-830.37445613
GGTATTC4600.029.93424645
GTGTAAG6400.028.2651212
GGGTAGT6800.027.79443724
GGTAGTC6950.027.19375625
TCTCGTA2600.026.99951638
GTACGGT400.00861225226.99951611
GAACATG18800.025.8536381
AACATGG19600.025.0739232
GTAGTCT7450.025.00626226
TGGTGTA7050.024.8931710
Produced by FastQC (version 0.11.5)