FastQCFastQC Report
Wed 25 May 2016
SRR2074884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889038
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG2650.034.64108354
ATTGGCG2900.033.5168321
TTGGCGT3250.030.73808522
AGCGGAT3600.029.24964335
TAGCGGA3500.028.54250734
CCGTCGT400.00861224826.99966834
GGTAGCG3850.026.64902532
TCGTATG1201.3460522E-924.74969740
GAACATG17250.024.5762141
TATGCCG1352.0190782E-1023.99970643
CGTATGC1502.910383E-1123.39971441
GCGGATG5150.022.54341336
GGTATCA50900.021.8036141
GTAGCGG4750.021.59973533
CGGATGA4750.021.59973537
GAATCAA12050.021.5123861
GGCGCCA4550.020.17557515
GCGCCAT4750.019.89449316
AGGTAGC5300.019.86768231
CGACCGT700.00553455819.28547721
Produced by FastQC (version 0.11.5)